BBa_K1072014
1
BBa_K1072014
pGAL1+ Flag + Brho + odr-10 + GFP + ADH1 Te
2013-09-09T11:00:00Z
2015-05-08T01:09:01Z
Caenorhabditis elegans olfactory receptor Odr-10
BBa_K1072013 is protein generator, including Odr-10 and GFP;
BBa_K1072013 encodes a diacetyl-sensing G-protein coupled receptor, 7-transmembrane receptors, which can be functionally expressed in S. cerevisiae. We add Brho signal peptide in C-terminal and link GFP coding region in N-terminal.
This part is an improvement of <bbpart>BBa_K211002</bbpart> and <bbpart>BBa_K775003</bbpart> made by 2009 Hongkong university and 2012 TU delft team, respectively. A Brho signal peptide and GFP have been added.
false
false
_1381_
0
16002
9
In stock
true
-
false
Junqi kuang, Yaqing du and Xihao liao
component2366566
1
BBa_K801012
component2366562
1
BBa_K1072010
component2366556
1
BBa_J63006
component2366564
1
BBa_E0040
component2366558
1
BBa_K1072008
component2366560
1
BBa_K1072009
annotation2366564
1
BBa_E0040
range2366564
1
1681
2400
annotation2366558
1
BBa_K1072008
range2366558
1
556
582
annotation2366562
1
BBa_K1072010
range2366562
1
655
1674
annotation2366566
1
BBa_K801012
range2366566
1
2409
2757
annotation2366560
1
BBa_K1072009
range2366560
1
589
648
annotation2366556
1
BBa_J63006
range2366556
1
1
549
BBa_K801012
1
BBa_K801012
ADH1 yeast terminator
2012-09-20T11:00:00Z
2015-05-08T01:13:24Z
Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
Released HQ 2013
ADH1 terminator of ''S. cerevisiae''
false
false
_1057_
0
13624
9
In stock
false
-
false
Georg Sch??tzinger
annotation2202145
1
ADH1-Terminator
range2202145
1
1
349
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_K1072010
1
BBa_K1072010
Odr-10(diacetyl)GPCR with optimized codon
2013-09-08T11:00:00Z
2015-05-08T01:09:01Z
Originate from the C. elegans.
Odr-10(diacetyl)GPCR encodes a diacetyl-sensing G-protein coupled receptor, originating from the C. elegans.
If BBa_K1072010 was transformed into the yeast, the pheromone receptors will be actived and coupled to a heterotrimeric G protein, Odr-10. pathway is composedof Gpa1p (Gα), Ste4p (Gβ) and Ste18p (Gγ) subunits.The Gβγ particle transmits the signal to a MAP kinase module and finally follow a inducible promotor FUS1, which will link a reproter, such as GFP, His3 or LacZ.
false
false
_1381_
0
16002
9
Not in stock
false
As discription of most paper, RNA of Odr-10 be used as plate and transform into receptor cells to express member protein successfully.
false
Junqi kuang, Xihao liao and Jianhui gong
annotation2357697
1
Odr-10 coding region
range2357697
1
1
1020
BBa_J63006
1
GAL1
yeast GAL1 promoter
2006-10-10T11:00:00Z
2015-08-31T01:56:26Z
genomic GAL1 locus
Released HQ 2013
GAL1 promoter from S. cerevisiae
false
true
_97_
0
545
97
In stock
false
unchanged from genomic sequence
true
Caroline Ajo-Franklin
BBa_K1072008
1
BBa_K1072008
Flag tag
2013-09-06T11:00:00Z
2015-05-08T01:09:01Z
synthesis
This part just is Flag tag.
false
false
_1381_
0
16002
9
Not in stock
false
-
false
Junqi kuang, Xihao liao and Jianhui gong
annotation2335360
1
Flag tag
range2335360
1
1
27
BBa_K1072009
1
BBa_K1072009
Bovine rhodopsin signalling pipetide
2013-09-06T11:00:00Z
2015-05-08T01:09:01Z
Systhesis
BBa_K1072009 is the bovine rhodopsin signalling pipetide with optimized codon.
false
false
_1381_
0
16002
9
Not in stock
false
Bovine rhodopsin signalling pipetide guide odr-10 to local S.cerevisiae membrane
false
Junqi kuang, Xihao liao and Jianhui gong
annotation2335361
1
bovine rhodopsin signalling peptide
range2335361
1
1
60
BBa_K801012_sequence
1
agctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg
BBa_J63006_sequence
1
ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggag
BBa_K1072014_sequence
1
ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagatggattacaaagatgatgatgataagtactagatgaatggtactgaaggtcctaacttctatgtccctttctcaaataagactggtgtcgtttactagatgtctggtgaattgtggataaccttagttgatactgctgacatcgtcggtgtaaccttgactttttgtgtaaacatagttttgttgggtttgttgaagactagaggtaaaaatttgggtacatacaagtacttaatggcatttttctccgtttttagtattttctatgccatcatcgagtttattttaagacctataatgcatatcgaaaacactacatttttcttgatctccagaaagagattcaattacagtaccaagttgggtaaaattaattcagctttctactgtgcatgcttcgccacttcttttgttgtctcaggtgtccacttcgtatatagatactttgcaacctgtaagcctaatttgttgagattgttcaacttaccaactttgttgttgtggcctttgggttgctccgttccagtcacaatgtgggcctctgtttcatactttttgtacccagatacagaatataccgaagctgctgttacaaacgtcttgaacaaccattacaactggatcaaaaaggaaaacgtcagttacatcgcatacgtatactaccaatacgaaaacggtgttagacatatatatttgaaaaatttgttgggttgtttcgtacactacttcgttatgtctatgacattcgtagttatgttctattgcggttacgctacttggaaaacaatgaacgaacacaaggatgtttcagacagaaccagagctttgcaaaagcaattgtttaaagcattagttttgcaaacattgatccctaccattttcatgtatgccccaacaggtgttatgtttattgctccatttttcgatgtcaatttgaacgccaacgctaacttcatcgttttctgttcctttttgtacccaggtttagatcctttgatcttgatcttgatcataagagacttcagaagaactatttttaatttcttgtgcggtaaaaagaacagtgtcgatgaatccagatccacaaccagagccaacttatcccaagtaccaacctaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagagctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K1072008_sequence
1
atggattacaaagatgatgatgataag
BBa_K1072010_sequence
1
atgtctggtgaattgtggataaccttagttgatactgctgacatcgtcggtgtaaccttgactttttgtgtaaacatagttttgttgggtttgttgaagactagaggtaaaaatttgggtacatacaagtacttaatggcatttttctccgtttttagtattttctatgccatcatcgagtttattttaagacctataatgcatatcgaaaacactacatttttcttgatctccagaaagagattcaattacagtaccaagttgggtaaaattaattcagctttctactgtgcatgcttcgccacttcttttgttgtctcaggtgtccacttcgtatatagatactttgcaacctgtaagcctaatttgttgagattgttcaacttaccaactttgttgttgtggcctttgggttgctccgttccagtcacaatgtgggcctctgtttcatactttttgtacccagatacagaatataccgaagctgctgttacaaacgtcttgaacaaccattacaactggatcaaaaaggaaaacgtcagttacatcgcatacgtatactaccaatacgaaaacggtgttagacatatatatttgaaaaatttgttgggttgtttcgtacactacttcgttatgtctatgacattcgtagttatgttctattgcggttacgctacttggaaaacaatgaacgaacacaaggatgtttcagacagaaccagagctttgcaaaagcaattgtttaaagcattagttttgcaaacattgatccctaccattttcatgtatgccccaacaggtgttatgtttattgctccatttttcgatgtcaatttgaacgccaacgctaacttcatcgttttctgttcctttttgtacccaggtttagatcctttgatcttgatcttgatcataagagacttcagaagaactatttttaatttcttgtgcggtaaaaagaacagtgtcgatgaatccagatccacaaccagagccaacttatcccaagtaccaacctaa
BBa_K1072009_sequence
1
atgaatggtactgaaggtcctaacttctatgtccctttctcaaataagactggtgtcgtt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z