BBa_K1072008 1 BBa_K1072008 Flag tag 2013-09-06T11:00:00Z 2015-05-08T01:09:01Z synthesis This part just is Flag tag. false false _1381_ 0 16002 9 Not in stock false - false Junqi kuang, Xihao liao and Jianhui gong annotation2335360 1 Flag tag range2335360 1 1 27 BBa_K1072010 1 BBa_K1072010 Odr-10(diacetyl)GPCR with optimized codon 2013-09-08T11:00:00Z 2015-05-08T01:09:01Z Originate from the C. elegans. Odr-10(diacetyl)GPCR encodes a diacetyl-sensing G-protein coupled receptor, originating from the C. elegans. If BBa_K1072010 was transformed into the yeast, the pheromone receptors will be actived and coupled to a heterotrimeric G protein, Odr-10. pathway is composedof Gpa1p (Gα), Ste4p (Gβ) and Ste18p (Gγ) subunits.The Gβγ particle transmits the signal to a MAP kinase module and finally follow a inducible promotor FUS1, which will link a reproter, such as GFP, His3 or LacZ. false false _1381_ 0 16002 9 Not in stock false As discription of most paper, RNA of Odr-10 be used as plate and transform into receptor cells to express member protein successfully. false Junqi kuang, Xihao liao and Jianhui gong annotation2357697 1 Odr-10 coding region range2357697 1 1 1020 BBa_K1072015 1 BBa_K1072015 GAL1 + Brho +Flag + odr-10 +GFP +ADH1 2013-09-09T11:00:00Z 2015-05-08T01:09:01Z Caenorhabditis elegans olfactory receptor Odr-10 and kit plate This is a GPCR generator of Odr-10 linking Brho signal peptide and reporter, flag tag and GFP. BBa_K1072015 encodes a diacetyl-sensing G-protein coupled receptor, 7-transmembrane receptors, which can be functionally expressed in S. cerevisiae. This part is an improvement of BBa_K211002 and BBa_K775003 made by 2009 Hongkong university and 2012 TU delft team, respectively. A Brho signal peptide， flag tag and GFP have been added. false false _1381_ 0 16002 9 Not in stock false - false Junqi kuang, Yaqing du and Xihao liao component2366575 1 BBa_E0040 component2366569 1 BBa_K1072009 component2366571 1 BBa_K1072008 component2366573 1 BBa_K1072010 component2366577 1 BBa_K801012 component2366567 1 BBa_J63006 annotation2366571 1 BBa_K1072008 range2366571 1 622 648 annotation2366577 1 BBa_K801012 range2366577 1 2409 2757 annotation2366573 1 BBa_K1072010 range2366573 1 655 1674 annotation2366575 1 BBa_E0040 range2366575 1 1681 2400 annotation2366567 1 BBa_J63006 range2366567 1 1 549 annotation2366569 1 BBa_K1072009 range2366569 1 556 615 BBa_J63006 1 GAL1 yeast GAL1 promoter 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z genomic GAL1 locus Released HQ 2013 GAL1 promoter from S. cerevisiae false true _97_ 0 545 97 In stock false unchanged from genomic sequence true Caroline Ajo-Franklin BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K801012 1 BBa_K801012 ADH1 yeast terminator 2012-09-20T11:00:00Z 2015-05-08T01:13:24Z Extracted from genomic DNA of ''S. cerevisiae'' using PCR. Released HQ 2013 ADH1 terminator of ''S. cerevisiae'' false false _1057_ 0 13624 9 In stock false - false Georg Sch??tzinger annotation2202145 1 ADH1-Terminator range2202145 1 1 349 BBa_K1072009 1 BBa_K1072009 Bovine rhodopsin signalling pipetide 2013-09-06T11:00:00Z 2015-05-08T01:09:01Z Systhesis BBa_K1072009 is the bovine rhodopsin signalling pipetide with optimized codon. false false _1381_ 0 16002 9 Not in stock false Bovine rhodopsin signalling pipetide guide odr-10 to local S.cerevisiae membrane false Junqi kuang, Xihao liao and Jianhui gong annotation2335361 1 bovine rhodopsin signalling peptide range2335361 1 1 60 BBa_K1072015_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagatgaatggtactgaaggtcctaacttctatgtccctttctcaaataagactggtgtcgtttactagatggattacaaagatgatgatgataagtactagatgtctggtgaattgtggataaccttagttgatactgctgacatcgtcggtgtaaccttgactttttgtgtaaacatagttttgttgggtttgttgaagactagaggtaaaaatttgggtacatacaagtacttaatggcatttttctccgtttttagtattttctatgccatcatcgagtttattttaagacctataatgcatatcgaaaacactacatttttcttgatctccagaaagagattcaattacagtaccaagttgggtaaaattaattcagctttctactgtgcatgcttcgccacttcttttgttgtctcaggtgtccacttcgtatatagatactttgcaacctgtaagcctaatttgttgagattgttcaacttaccaactttgttgttgtggcctttgggttgctccgttccagtcacaatgtgggcctctgtttcatactttttgtacccagatacagaatataccgaagctgctgttacaaacgtcttgaacaaccattacaactggatcaaaaaggaaaacgtcagttacatcgcatacgtatactaccaatacgaaaacggtgttagacatatatatttgaaaaatttgttgggttgtttcgtacactacttcgttatgtctatgacattcgtagttatgttctattgcggttacgctacttggaaaacaatgaacgaacacaaggatgtttcagacagaaccagagctttgcaaaagcaattgtttaaagcattagttttgcaaacattgatccctaccattttcatgtatgccccaacaggtgttatgtttattgctccatttttcgatgtcaatttgaacgccaacgctaacttcatcgttttctgttcctttttgtacccaggtttagatcctttgatcttgatcttgatcataagagacttcagaagaactatttttaatttcttgtgcggtaaaaagaacagtgtcgatgaatccagatccacaaccagagccaacttatcccaagtaccaacctaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagagctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg BBa_K801012_sequence 1 agctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg BBa_J63006_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggag BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1072008_sequence 1 atggattacaaagatgatgatgataag BBa_K1072010_sequence 1 atgtctggtgaattgtggataaccttagttgatactgctgacatcgtcggtgtaaccttgactttttgtgtaaacatagttttgttgggtttgttgaagactagaggtaaaaatttgggtacatacaagtacttaatggcatttttctccgtttttagtattttctatgccatcatcgagtttattttaagacctataatgcatatcgaaaacactacatttttcttgatctccagaaagagattcaattacagtaccaagttgggtaaaattaattcagctttctactgtgcatgcttcgccacttcttttgttgtctcaggtgtccacttcgtatatagatactttgcaacctgtaagcctaatttgttgagattgttcaacttaccaactttgttgttgtggcctttgggttgctccgttccagtcacaatgtgggcctctgtttcatactttttgtacccagatacagaatataccgaagctgctgttacaaacgtcttgaacaaccattacaactggatcaaaaaggaaaacgtcagttacatcgcatacgtatactaccaatacgaaaacggtgttagacatatatatttgaaaaatttgttgggttgtttcgtacactacttcgttatgtctatgacattcgtagttatgttctattgcggttacgctacttggaaaacaatgaacgaacacaaggatgtttcagacagaaccagagctttgcaaaagcaattgtttaaagcattagttttgcaaacattgatccctaccattttcatgtatgccccaacaggtgttatgtttattgctccatttttcgatgtcaatttgaacgccaacgctaacttcatcgttttctgttcctttttgtacccaggtttagatcctttgatcttgatcttgatcataagagacttcagaagaactatttttaatttcttgtgcggtaaaaagaacagtgtcgatgaatccagatccacaaccagagccaacttatcccaagtaccaacctaa BBa_K1072009_sequence 1 atgaatggtactgaaggtcctaacttctatgtccctttctcaaataagactggtgtcgtt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z