LOCUS BBa_K1072017 4169 bp DNA linear 25-Nov-2024 DEFINITION pGAL1 + Flag + odr-10 + GFP + ADH1Te + pFUS1 + BFP + ADH1Te ACCESSION BBa_K1072017 VERSION BBa_K1072017.1 FEATURES Location/Qualifiers promoter 1..549 /label=BBa_J63006 misc_feature 556..582 /label=Flag tag misc_feature 556..582 /label=BBa_K1072008 CDS 589..1608 /label=Odr-10 coding region misc_feature 589..1608 /label=BBa_K1072010 CDS 1615..2334 /label=GFP protein CDS 1615..2334 /label=BBa_E0040 misc_feature 2343..2691 /label=ADH1-Terminator terminator 2343..2691 /label=BBa_K801012 misc_feature 1..2691 /label=BBa_K1072013 promoter 2700..3137 /label=Fus1 promoter 2700..3137 /label=BBa_K1072023 CDS 3144..3809 /label=amilCP CDS 3144..3812 /label=BBa_K592009 misc_feature 3821..4169 /label=ADH1-Terminator terminator 3821..4169 /label=BBa_K801012 misc_feature 2700..4169 /label=BBa_K1072020 ORIGIN 1 ccccattatc ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg 61 ctcattgcta tattgaagta cggattagaa gccgccgagc gggtgacagc cctccgaagg 121 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc 181 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag 241 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa 301 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa 361 gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac 421 taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa 481 caaaaaattg ttaatatacc tctatacttt aacgtcaagg aggaaactag acccgccgcc 541 accatggagt actagatgga ttacaaagat gatgatgata agtactagat gtctggtgaa 601 ttgtggataa ccttagttga tactgctgac atcgtcggtg taaccttgac tttttgtgta 661 aacatagttt tgttgggttt gttgaagact agaggtaaaa atttgggtac atacaagtac 721 ttaatggcat ttttctccgt ttttagtatt ttctatgcca tcatcgagtt tattttaaga 781 cctataatgc atatcgaaaa cactacattt ttcttgatct ccagaaagag attcaattac 841 agtaccaagt tgggtaaaat taattcagct ttctactgtg catgcttcgc cacttctttt 901 gttgtctcag gtgtccactt cgtatataga tactttgcaa cctgtaagcc taatttgttg 961 agattgttca acttaccaac tttgttgttg tggcctttgg gttgctccgt tccagtcaca 1021 atgtgggcct ctgtttcata ctttttgtac ccagatacag aatataccga agctgctgtt 1081 acaaacgtct tgaacaacca ttacaactgg atcaaaaagg aaaacgtcag ttacatcgca 1141 tacgtatact accaatacga aaacggtgtt agacatatat atttgaaaaa tttgttgggt 1201 tgtttcgtac actacttcgt tatgtctatg acattcgtag ttatgttcta ttgcggttac 1261 gctacttgga aaacaatgaa cgaacacaag gatgtttcag acagaaccag agctttgcaa 1321 aagcaattgt ttaaagcatt agttttgcaa acattgatcc ctaccatttt catgtatgcc 1381 ccaacaggtg ttatgtttat tgctccattt ttcgatgtca atttgaacgc caacgctaac 1441 ttcatcgttt tctgttcctt tttgtaccca ggtttagatc ctttgatctt gatcttgatc 1501 ataagagact tcagaagaac tatttttaat ttcttgtgcg gtaaaaagaa cagtgtcgat 1561 gaatccagat ccacaaccag agccaactta tcccaagtac caacctaata ctagatgcgt 1621 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt 1681 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt 1741 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact 1801 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac 1861 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat 1921 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga 1981 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa 2041 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa 2101 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat 2161 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc 2221 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag 2281 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag 2341 agagctttgg acttcttcgc cagaggtttg gtcaagtctc caatcaaggt tgtcggcttg 2401 tctaccttgc cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg tagatacgtt 2461 gttgacactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt 2521 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc 2581 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc 2641 gctcttattg accacacctc taccggccgg tcgaaattcc cctaccctat gtactagagt 2701 gaacgatgat tcagttcgcc ttctatcctt tgtttacgta tttgtttata tatataactt 2761 tatttttttt tattaattgg gctgcaagac aattttgttg tcagtgatgc ctcaatcctt 2821 cttttgcttc catatttacc atgtggaccc tttcaaaaca gagttgtatc tctggaggat 2881 gccctttttg acgtattgaa tggcataatt gcactgtcac ttttcgcgct gtctcatttt 2941 ggtgcgatga tgaaacaaac atgaaacgtc tgtaatttga aacaaataac gtaattctcg 3001 ggattggttt tatttaaatg acaatgtaag agtggctttg taaggtatgt gttgctctta 3061 aaatatttgg atacgacatc ctttatcttt tttcctttaa gagcaggata taagccatca 3121 agtttctgaa aatcaaatac tagatgagtg tgatcgctaa acaaatgacc tacaaggttt 3181 atatgtcagg cacggtcaat ggacactact ttgaggtcga aggcgatgga aaaggtaagc 3241 cctacgaggg ggagcagacg gtaaagctca ctgtcaccaa gggcggacct ctgccatttg 3301 cttgggatat tttatcacca cagtgtcagt acggaagcat accattcacc aagtaccctg 3361 aagacatccc tgactatgta aagcagtcat tcccggaggg ctatacatgg gagaggatca 3421 tgaactttga agatggtgca gtgtgtactg tcagcaatga ttccagcatc caaggcaact 3481 gtttcatcta ccatgtcaag ttctctggtt tgaactttcc tcccaatgga cctgtcatgc 3541 agaagaagac acagggctgg gaacccaaca ctgagcgtct ctttgcacga gatggaatgc 3601 tgctaggaaa caactttatg gctctgaagt tagaaggagg cggtcactat ttgtgtgaat 3661 ttaaaactac ttacaaggca aagaagcctg tgaagatgcc agggtatcac tatgttgacc 3721 gcaaactgga tgtaaccaat cacaacaagg attacacttc ggttgagcag tgtgaaattt 3781 ccattgcacg caaacctgtg gtcgcctaat aatactagag agctttggac ttcttcgcca 3841 gaggtttggt caagtctcca atcaaggttg tcggcttgtc taccttgcca gaaatttacg 3901 aaaagatgga aaagggtcaa atcgttggta gatacgttgt tgacacttct aaataagcga 3961 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca 4021 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc 4081 tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta 4141 ccggccggtc gaaattcccc taccctatg //