LOCUS BBa_K1072019 4235 bp DNA linear 25-Nov-2024 DEFINITION pGAL1 + Brho + Flag + odr-10 + GFP +ADH1 Te + pFUS1 + BFP + ADH1 Te ACCESSION BBa_K1072019 VERSION BBa_K1072019.1 FEATURES Location/Qualifiers promoter 1..549 /label=BBa_J63006 CDS 556..615 /label=bovine rhodopsin signalling peptide misc_feature 556..615 /label=BBa_K1072009 misc_feature 622..648 /label=Flag tag misc_feature 622..648 /label=BBa_K1072008 CDS 655..1674 /label=Odr-10 coding region misc_feature 655..1674 /label=BBa_K1072010 CDS 1681..2400 /label=GFP protein CDS 1681..2400 /label=BBa_E0040 misc_feature 2409..2757 /label=ADH1-Terminator terminator 2409..2757 /label=BBa_K801012 misc_feature 1..2757 /label=BBa_K1072015 promoter 2766..3203 /label=Fus1 promoter 2766..3203 /label=BBa_K1072023 CDS 3210..3875 /label=amilCP CDS 3210..3878 /label=BBa_K592009 misc_feature 3887..4235 /label=ADH1-Terminator terminator 3887..4235 /label=BBa_K801012 misc_feature 2766..4235 /label=BBa_K1072020 ORIGIN 1 ccccattatc ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg 61 ctcattgcta tattgaagta cggattagaa gccgccgagc gggtgacagc cctccgaagg 121 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc 181 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag 241 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa 301 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa 361 gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac 421 taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa 481 caaaaaattg ttaatatacc tctatacttt aacgtcaagg aggaaactag acccgccgcc 541 accatggagt actagatgaa tggtactgaa ggtcctaact tctatgtccc tttctcaaat 601 aagactggtg tcgtttacta gatggattac aaagatgatg atgataagta ctagatgtct 661 ggtgaattgt ggataacctt agttgatact gctgacatcg tcggtgtaac cttgactttt 721 tgtgtaaaca tagttttgtt gggtttgttg aagactagag gtaaaaattt gggtacatac 781 aagtacttaa tggcattttt ctccgttttt agtattttct atgccatcat cgagtttatt 841 ttaagaccta taatgcatat cgaaaacact acatttttct tgatctccag aaagagattc 901 aattacagta ccaagttggg taaaattaat tcagctttct actgtgcatg cttcgccact 961 tcttttgttg tctcaggtgt ccacttcgta tatagatact ttgcaacctg taagcctaat 1021 ttgttgagat tgttcaactt accaactttg ttgttgtggc ctttgggttg ctccgttcca 1081 gtcacaatgt gggcctctgt ttcatacttt ttgtacccag atacagaata taccgaagct 1141 gctgttacaa acgtcttgaa caaccattac aactggatca aaaaggaaaa cgtcagttac 1201 atcgcatacg tatactacca atacgaaaac ggtgttagac atatatattt gaaaaatttg 1261 ttgggttgtt tcgtacacta cttcgttatg tctatgacat tcgtagttat gttctattgc 1321 ggttacgcta cttggaaaac aatgaacgaa cacaaggatg tttcagacag aaccagagct 1381 ttgcaaaagc aattgtttaa agcattagtt ttgcaaacat tgatccctac cattttcatg 1441 tatgccccaa caggtgttat gtttattgct ccatttttcg atgtcaattt gaacgccaac 1501 gctaacttca tcgttttctg ttcctttttg tacccaggtt tagatccttt gatcttgatc 1561 ttgatcataa gagacttcag aagaactatt tttaatttct tgtgcggtaa aaagaacagt 1621 gtcgatgaat ccagatccac aaccagagcc aacttatccc aagtaccaac ctaatactag 1681 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt 1741 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga 1801 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt 1861 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag 1921 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc 1981 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt 2041 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa 2101 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga 2161 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac 2221 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac 2281 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt 2341 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa 2401 tactagagag ctttggactt cttcgccaga ggtttggtca agtctccaat caaggttgtc 2461 ggcttgtcta ccttgccaga aatttacgaa aagatggaaa agggtcaaat cgttggtaga 2521 tacgttgttg acacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa 2581 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga 2641 aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat 2701 gaggtcgctc ttattgacca cacctctacc ggccggtcga aattccccta ccctatgtac 2761 tagagtgaac gatgattcag ttcgccttct atcctttgtt tacgtatttg tttatatata 2821 taactttatt tttttttatt aattgggctg caagacaatt ttgttgtcag tgatgcctca 2881 atccttcttt tgcttccata tttaccatgt ggaccctttc aaaacagagt tgtatctctg 2941 gaggatgccc tttttgacgt attgaatggc ataattgcac tgtcactttt cgcgctgtct 3001 cattttggtg cgatgatgaa acaaacatga aacgtctgta atttgaaaca aataacgtaa 3061 ttctcgggat tggttttatt taaatgacaa tgtaagagtg gctttgtaag gtatgtgttg 3121 ctcttaaaat atttggatac gacatccttt atcttttttc ctttaagagc aggatataag 3181 ccatcaagtt tctgaaaatc aaatactaga tgagtgtgat cgctaaacaa atgacctaca 3241 aggtttatat gtcaggcacg gtcaatggac actactttga ggtcgaaggc gatggaaaag 3301 gtaagcccta cgagggggag cagacggtaa agctcactgt caccaagggc ggacctctgc 3361 catttgcttg ggatatttta tcaccacagt gtcagtacgg aagcatacca ttcaccaagt 3421 accctgaaga catccctgac tatgtaaagc agtcattccc ggagggctat acatgggaga 3481 ggatcatgaa ctttgaagat ggtgcagtgt gtactgtcag caatgattcc agcatccaag 3541 gcaactgttt catctaccat gtcaagttct ctggtttgaa ctttcctccc aatggacctg 3601 tcatgcagaa gaagacacag ggctgggaac ccaacactga gcgtctcttt gcacgagatg 3661 gaatgctgct aggaaacaac tttatggctc tgaagttaga aggaggcggt cactatttgt 3721 gtgaatttaa aactacttac aaggcaaaga agcctgtgaa gatgccaggg tatcactatg 3781 ttgaccgcaa actggatgta accaatcaca acaaggatta cacttcggtt gagcagtgtg 3841 aaatttccat tgcacgcaaa cctgtggtcg cctaataata ctagagagct ttggacttct 3901 tcgccagagg tttggtcaag tctccaatca aggttgtcgg cttgtctacc ttgccagaaa 3961 tttacgaaaa gatggaaaag ggtcaaatcg ttggtagata cgttgttgac acttctaaat 4021 aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg 4081 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact 4141 ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca 4201 cctctaccgg ccggtcgaaa ttcccctacc ctatg //