pGAL1 + Brho + Flag + odr-10 + GFP +ADH1 Te + pFUS1 + BFP + ADH1 Te
Caenorhabditis elegans olfactory receptor Odr-10 and kit plate
This is a GPCR generator of Odr-10 linking Brho signal peptide and reporter, flag tag and GFP. BBa_K1072015 encodes a diacetyl-sensing G-protein coupled receptor, 7-transmembrane receptors, which can be functionally expressed in S. cerevisiae. This part is an improvement of BBa_K211002 and BBa_K775003 made by 2009 Hongkong university and 2012 TU delft team, respectively. A Brho signal peptide， flag tag and GFP have been added.
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
GFP (mut3b) [note that this part does not have a barcode]
Junqi kuang, Xihao liao and Jianhui gong
BBa_K1072009 is the bovine rhodopsin signalling pipetide with optimized codon.
Bovine rhodopsin signalling pipetide guide odr-10 to local S.cerevisiae membrane
Odr-10(diacetyl)GPCR with optimized codon
Odr-10(diacetyl)GPCR encodes a diacetyl-sensing G-protein coupled receptor, originating from the C. elegans. If BBa_K1072010 was transformed into the yeast, the pheromone receptors will be actived and coupled to a heterotrimeric G protein, Odr-10. pathway is composedof Gpa1p (Gα), Ste4p (Gβ) and Ste18p (Gγ) subunits.The Gβγ particle transmits the signal to a MAP kinase module and finally follow a inducible promotor FUS1, which will link a reproter, such as GFP, His3 or LacZ.
As discription of most paper, RNA of Odr-10 be used as plate and transform into receptor cells to express member protein successfully.
BBa_K1072023 is a induced promotor, originating from S.cerevisiae.
Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
This chromoprotein, amilCP, naturally exhibits very strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. This DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of one illegal internal restriction site (EcoRI).
Illegal internal restriction site had to be removed (EcoRI).
Conversion of the iGEM parts registry to SBOL2.1