BBa_R0071
1
RhlR+C4
Promoter (RhlR & C4-HSL regulated)
2004-01-24T12:00:00Z
2015-05-08T01:14:15Z
<i>Pseudomonas aeruginosa</i> rhlAB promoter
Released HQ 2013
Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer.
<p>
This is the natural sequence taken from Pseudomonas aeruginosa.
<p>
Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
false
false
_1_
0
24
7
In stock
false
The -10 and -35 sites are labeled as identified in [Pearson97].
<P>
The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
true
Ronny Krashinsky
annotation297104
1
-10
range297104
1
42
47
annotation297105
1
start
range297105
1
53
53
annotation297102
1
RhlR
range297102
1
1
20
annotation297103
1
-35
range297103
1
19
24
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation7027
1
BBa_B0032
range7027
1
1
13
BBa_K1073001
1
pRhl + RBS
Inducible promoter of the rhl quorum sensing system with RBS
2013-09-15T11:00:00Z
2015-05-08T01:09:01Z
Combined construct from BBa_R0071 and BBa_B0032, which were taken from the 2013 distribution kit.
This construct is derived from BBa_R0071 and B0032. It combines the rhl induced promoter with an RBS and can be used to clone it in front of a desired gene of interest, which shall be induced by rhl.
false
false
_1382_
0
15674
9
In stock
false
no considerations
false
iGEM Team Braunschweig 2013
component2346211
1
BBa_R0071
component2346213
1
BBa_B0032
annotation2346213
1
BBa_B0032
range2346213
1
62
74
annotation2346211
1
BBa_R0071
range2346211
1
1
53
BBa_K1073011
1
BBa_K1073011
Inducible ampicillin resistance cassette (regulated by RhlR + N-buturyl-HSL)
2013-09-15T11:00:00Z
2015-05-08T01:09:01Z
This part is a combination of BBa_K1073001 and BBa_K1073000, which themselves were constructed from biobricks of the 2013 distribution kit.
The promoter for this beta-lactonase is induced by N-(butyryl)-homoserine lactone. Therefore the expression of the ampicillin beta-lactonase is inducable by Rhl Inducer N-(butyryl)-homoserine lactone.
false
false
_1382_
0
15674
9
In stock
false
no considerations.
false
iGEM Team Braunschweig 2013
component2373103
1
BBa_K1073000
component2373102
1
BBa_K1073001
annotation2373103
1
BBa_K1073000
range2373103
1
81
944
annotation2373102
1
BBa_K1073001
range2373102
1
1
74
BBa_K1073000
1
ampR
ampicillin resistance
2013-09-15T11:00:00Z
2016-01-28T01:50:42Z
The part was derived from BBa_P1002 ampicillin resistance cassette. The sequence for the beta-lactamase was gained by PCR of the original BBa_P1002 with appropiate primers.
This sequence codes for a beta-lactamase, which degrades ampicillin. There is no RBS or other information attached, only the sequence for the enzyme plus prefix and suffix.
false
false
_1382_
4206
15674
9
Not in stock
false
The primer sequences can be found on iGEM Team Braunschweig's wiki.
false
iGEM Team Braunschweig 2013
BBa_K1073000_sequence
1
atgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataa
BBa_K1073001_sequence
1
tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagtcacacaggaaag
BBa_R0071_sequence
1
tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttc
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K1073011_sequence
1
tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagtcacacaggaaagtactagatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z