LOCUS       BBa_K1073019             823 bp    DNA     linear       22-Nov-2024
DEFINITION  Autoinducer synthetase RhlI ( producing N-butyryl-HSL) with RBS 
            and double terminator
ACCESSION   BBa_K1073019
VERSION     BBa_K1073019.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..622
                     /label=rhlL
     misc_feature    623..655
                     /label=LVA
     misc_feature    662..686
                     /label=Help:Barcodes
     CDS             20..686
                     /label=BBa_C0070
     stem_loop       706..749
                     /label=stem_loop
     misc_feature    695..774
                     /label=BBa_B0010
     terminator      695..774
                     /label=BBa_B0010
     stem_loop       790..809
                     /label=T7 TE
     misc_feature    816..816
                     /label=stop
     polyA_site      810..823
                     /label=polya
     misc_feature    783..823
                     /label=BBa_B0012
     terminator      783..823
                     /label=BBa_B0012
     terminator      695..823
                     /label=BBa_B0015
ORIGIN
        1 tcacacagga aagtactaga tgatcgaact gctgtccgaa tccctggaag gtctgtccgc
       61 tgctatgatc gctgaactgg gtcgttaccg tcaccaggtt ttcatcgaaa aactgggttg
      121 ggacgttgtt tccacctccc gtgttcgtga ccaggagttc gaccagttcg accacccgca
      181 gacccgttac atcgttgcta tgtcccgtca gggtatctgc ggttgcgctc gtctgctgcc
      241 gaccaccgac gcttacctgc tgaaagacgt tttcgcttac ctgtgctccg aaaccccgcc
      301 gtccgacccg tccgtttggg aactgtcccg ttacgctgct tccgctgctg acgacccgca
      361 gctggctatg aaaatcttct ggtcctccct ccagtgcgct tggtacctgg gtgcttcctc
      421 cgttgttgct gttaccacca ccgctatgga acgttacttc gttcgtaacg gtgttatcct
      481 ccagcgtctg ggtccgccgc agaaagttaa aggtgaaacc ctggttgcta tctccttccc
      541 ggcttaccag gaacgtggtc tggaaatgct gctgcgttac cacccggaat ggctccaggg
      601 tgttccgctg tccatggctg ttgctgcaaa cgacgaaaac tacgctttag tagcttaata
      661 actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct
      721 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      781 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//