LOCUS       BBa_K1073028            1938 bp    DNA     linear       07-Jun-2024
DEFINITION  Inducible ampicillin resistance cassette + RhlR expression cassette
ACCESSION   BBa_K1073028
VERSION     BBa_K1073028.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     promoter        19..24
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     promoter        1..53
                     /label=BBa_R0071
     RBS             62..74
                     /label=RBS-3\Weak
     RBS             68..71
                     /label=RBS
     misc_feature    62..74
                     /label=BBa_B0032
     RBS             62..74
                     /label=BBa_B0032
     promoter        1..74
                     /label=BBa_K1073001
     CDS             81..944
                     /label=BBa_K1073000
     misc_feature    1..944
                     /label=BBa_K1073011
     stem_loop       964..1007
                     /label=stem_loop
     misc_feature    953..1032
                     /label=BBa_B0010
     terminator      953..1032
                     /label=BBa_B0010
     stem_loop       1048..1067
                     /label=T7 TE
     misc_feature    1074..1074
                     /label=stop
     polyA_site      1068..1081
                     /label=polya
     misc_feature    1041..1081
                     /label=BBa_B0012
     terminator      1041..1081
                     /label=BBa_B0012
     terminator      953..1081
                     /label=BBa_B0015
     promoter        1090..1124
                     /label=BBa_J23100
     RBS             1133..1145
                     /label=RBS-3\Weak
     RBS             1139..1142
                     /label=RBS
     misc_feature    1133..1145
                     /label=BBa_B0032
     RBS             1133..1145
                     /label=BBa_B0032
     promoter        1090..1145
                     /label=BBa_K1073006
     CDS             1152..1874
                     /label=rhlR
     misc_feature    1875..1907
                     /label=LVA
     misc_feature    1914..1938
                     /label=Help:Barcodes
     CDS             1152..1938
                     /label=BBa_C0071
     misc_feature    1090..1938
                     /label=BBa_K1073014
ORIGIN
        1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga
       61 gtcacacagg aaagtactag atgagtattc aacatttccg tgtcgccctt attccctttt
      121 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      181 ccgaagatca gttgggtgca cgtgtgggtt acatcgaact ggacctcaac agcggtaaga
      241 ttcttgagag ttttcgcccc gaagaacgtt tcccaatgat gagcactttt aaagttctgc
      301 tctgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      361 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggacg
      421 gcatgacagt acgcgaatta tgcagcgctg ccataaccat gagtgataac acggcggcca
      481 acttacttct gacaacgatc ggaggaccga aggagcttac cgcttttttg cacaacatgg
      541 gtgatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      601 acgagcgtga caccacgatg cctgtagcta tggcaacaac gttgcgcaaa ctcttaactg
      661 gcgaacttct tactctcgct tcccggcaac aattaataga ctggatggag gcggataaag
      721 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      781 gagccggtga gcgtgggtcc cgcggtatta ttgcagccct ggggccagat ggtaagccct
      841 cccgtatcgt agttatctac acgacgggga gccaggcaac tatggacgaa cgtaatcgcc
      901 agatcgctga gataggtgcc tccctgatta agcattggta ataatactag agccaggcat
      961 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1021 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1081 atactagagt tgacggctag ctcagtccta ggtacagtgc tagctactag agtcacacag
     1141 gaaagtacta gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg
     1201 agatgcagcc gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc
     1261 gcctgggctt cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga
     1321 agaccgaggt ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact
     1381 acggggccgt ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga
     1441 gcgacagcct gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct
     1501 gtgtcggcgc gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg
     1561 cgcgcgacca gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt
     1621 gcatgatcga gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca
     1681 acccggtctg cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga
     1741 gttccgggga aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca
     1801 agaacatcca gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg
     1861 cgctgggtct catcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat
     1921 agtgctagtg tagatcac
//