LOCUS       BBa_K1073029            1934 bp    DNA     linear       22-Nov-2024
DEFINITION  Inducible ampicillin resistance cassette + LuxR expression cassette
ACCESSION   BBa_K1073029
VERSION     BBa_K1073029.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     promoter        1..76
                     /label=BBa_K1073002
     CDS             83..946
                     /label=BBa_K1073000
     misc_feature    1..946
                     /label=BBa_K1073012
     stem_loop       966..1009
                     /label=stem_loop
     misc_feature    955..1034
                     /label=BBa_B0010
     terminator      955..1034
                     /label=BBa_B0010
     stem_loop       1050..1069
                     /label=T7 TE
     misc_feature    1076..1076
                     /label=stop
     polyA_site      1070..1083
                     /label=polya
     misc_feature    1043..1083
                     /label=BBa_B0012
     terminator      1043..1083
                     /label=BBa_B0012
     terminator      955..1083
                     /label=BBa_B0015
     promoter        1092..1126
                     /label=BBa_J23100
     RBS             1135..1147
                     /label=RBS-3\Weak
     RBS             1141..1144
                     /label=RBS
     misc_feature    1135..1147
                     /label=BBa_B0032
     RBS             1135..1147
                     /label=BBa_B0032
     promoter        1092..1147
                     /label=BBa_K1073006
     misc_feature    1154..1155
                     /label=prefix
     misc_feature    1327..1327
                     /label=T
     misc_feature    1645..1645
                     /label=A
     CDS             1154..1903
                     /label=luxR
     misc_feature    1154..1909
                     /label=BBa_C0062
     misc_feature    1910..1934
                     /label=Help:Barcodes
     CDS             1154..1909
                     /label=BBa_C0062
     misc_feature    1092..1934
                     /label=BBa_K1073015
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagtcacaca ggaaagtact agatgagtat tcaacatttc cgtgtcgccc ttattccctt
      121 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      181 tgccgaagat cagttgggtg cacgtgtggg ttacatcgaa ctggacctca acagcggtaa
      241 gattcttgag agttttcgcc ccgaagaacg tttcccaatg atgagcactt ttaaagttct
      301 gctctgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      361 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      421 cggcatgaca gtacgcgaat tatgcagcgc tgccataacc atgagtgata acacggcggc
      481 caacttactt ctgacaacga tcggaggacc gaaggagctt accgcttttt tgcacaacat
      541 gggtgatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      601 cgacgagcgt gacaccacga tgcctgtagc tatggcaaca acgttgcgca aactcttaac
      661 tggcgaactt cttactctcg cttcccggca acaattaata gactggatgg aggcggataa
      721 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
      781 tggagccggt gagcgtgggt cccgcggtat tattgcagcc ctggggccag atggtaagcc
      841 ctcccgtatc gtagttatct acacgacggg gagccaggca actatggacg aacgtaatcg
      901 ccagatcgct gagataggtg cctccctgat taagcattgg taataatact agagccaggc
      961 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1021 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1081 atatactaga gttgacggct agctcagtcc taggtacagt gctagctact agagtcacac
     1141 aggaaagtac tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat
     1201 taaagcttgt agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt
     1261 acattgtgaa tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat
     1321 ttcaatccta gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat
     1381 aaaatatgat cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat
     1441 atttgaaaac aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc
     1501 aggtcttatc actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct
     1561 tagttttgca cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat
     1621 gaacatacca ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa
     1681 taataaatca aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga
     1741 aggaaaaagc tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt
     1801 ccatttaacc aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa
     1861 agcaatttta acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg
     1921 ctagtgtaga tcac
//