##gff-version 3
##sequence-region BBa_K1073029 1 1934
BBa_K1073029	.	engineered_region	1092	1934	.	+	0	ID=BBa_K1073015;Name=BBa_K1073015
BBa_K1073029	.	CDS	1154	1909	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K1073015
BBa_K1073029	.	point_mutation	1645	1645	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1073029	.	CDS	1154	1903	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1073029	.	engineered_tag	1910	1934	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1073029	.	engineered_region	1154	1909	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1073029	.	point_mutation	1327	1327	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1073029	.	engineered_region	1154	1155	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1073029	.	promoter	1092	1147	.	+	0	ID=BBa_K1073006;Name=BBa_K1073006;Parent=BBa_K1073015
BBa_K1073029	.	ribosome_entry_site	1135	1147	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1073006
BBa_K1073029	.	ribosome_entry_site	1141	1144	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1073029	.	engineered_region	1135	1147	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1073029	.	ribosome_entry_site	1135	1147	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1073029	.	promoter	1092	1126	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1073006
BBa_K1073029	.	engineered_region	1	946	.	+	0	ID=BBa_K1073012;Name=BBa_K1073012
BBa_K1073029	.	CDS	83	946	.	+	0	ID=BBa_K1073000;Name=BBa_K1073000;Parent=BBa_K1073012
BBa_K1073029	.	promoter	1	76	.	+	0	ID=BBa_K1073002;Name=BBa_K1073002;Parent=BBa_K1073012
BBa_K1073029	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K1073002
BBa_K1073029	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1073029	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1073029	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1073029	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1073029	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1073029	.	ribosome_entry_site	64	76	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1073002
BBa_K1073029	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1073029	.	engineered_region	64	76	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1073029	.	ribosome_entry_site	64	76	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1073029	.	terminator	955	1083	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1073029	.	terminator	1043	1083	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1073029	.	engineered_region	1043	1083	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1073029	.	polyA_site	1070	1083	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1073029	.	stem_loop	1050	1069	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1073029	.	stop_codon	1076	1076	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1073029	.	terminator	955	1034	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1073029	.	engineered_region	955	1034	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1073029	.	stem_loop	966	1009	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K1073029
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtac
tagatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccag
aaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcgg
taagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggta
ttatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcac
cagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacac
ggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgta
actcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcta
tggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatgga
ggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggt
gagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacgg
ggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctca
gtcctaggtacagtgctagctactagagtcacacaggaaagtactagatgaaaaacataaatgccgacgacacatacag
aataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacat
tgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattacccta
aaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattc
accaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatca
ggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaa
aagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataatta
tcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgc
gaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgc
aaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactt
taaaaattaataacactgatagtgctagtgtagatcac