##gff-version 3
##sequence-region BBa_K1073030 1 2005
BBa_K1073030	.	engineered_region	1	1017	.	+	0	ID=BBa_K1073013;Name=BBa_K1073013
BBa_K1073030	.	CDS	154	1017	.	+	0	ID=BBa_K1073000;Name=BBa_K1073000;Parent=BBa_K1073013
BBa_K1073030	.	promoter	1	147	.	+	0	ID=BBa_K1073003;Name=BBa_K1073003;Parent=BBa_K1073013
BBa_K1073030	.	ribosome_entry_site	135	147	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1073003
BBa_K1073030	.	ribosome_entry_site	141	144	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1073030	.	ribosome_entry_site	135	147	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1073030	.	engineered_region	135	147	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1073030	.	promoter	1	126	.	+	0	ID=BBa_K091117;Name=BBa_K091117;Parent=BBa_K1073003
BBa_K1073030	.	sequence_feature	113	118	.	+	0	ID=annotation1964074;Name= -10 Region;Parent=BBa_K091117
BBa_K1073030	.	start_codon	125	125	.	+	0	ID=annotation1964075;Name=Primary Transcriptional Start;Parent=BBa_K091117
BBa_K1073030	.	sequence_feature	86	91	.	+	0	ID=annotation1964072;Name=-35 Region;Parent=BBa_K091117
BBa_K1073030	.	non_covalent_binding_site	75	93	.	+	0	ID=annotation1964073;Name=LasR Binding Site;Parent=BBa_K091117
BBa_K1073030	.	engineered_region	1163	2005	.	+	0	ID=BBa_K1073016;Name=BBa_K1073016
BBa_K1073030	.	promoter	1163	1218	.	+	0	ID=BBa_K1073006;Name=BBa_K1073006;Parent=BBa_K1073016
BBa_K1073030	.	promoter	1163	1197	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1073006
BBa_K1073030	.	ribosome_entry_site	1206	1218	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1073006
BBa_K1073030	.	ribosome_entry_site	1212	1215	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1073030	.	ribosome_entry_site	1206	1218	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1073030	.	engineered_region	1206	1218	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1073030	.	CDS	1225	2005	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_K1073016
BBa_K1073030	.	engineered_tag	1981	2005	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K1073030	.	tag	1942	1974	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K1073030	.	sequence_alteration	1704	1704	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K1073030	.	CDS	1225	1941	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K1073030	.	terminator	1026	1154	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1073030	.	terminator	1026	1105	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1073030	.	stem_loop	1037	1080	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1073030	.	engineered_region	1026	1105	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1073030	.	terminator	1114	1154	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1073030	.	engineered_region	1114	1154	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1073030	.	stop_codon	1147	1147	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1073030	.	polyA_site	1141	1154	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1073030	.	stem_loop	1121	1140	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_K1073030
tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatcta
tctcatttgctagttataaaattatgaaatttgtataaattcttcagtactagagtcacacaggaaagtactagatgag
tattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctg
gtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattc
ttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccg
tattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcaca
gaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggcca
acttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgcct
tgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaaca
acgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggata
aagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgg
gtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccag
gcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctagg
tacagtgctagctactagagtcacacaggaaagtactagatggccttggttgacggttttcttgagctggaacgctcaa
gtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgtt
gcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgg
gctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatct
accagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgca
tggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagag
tcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtca
gcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatc
ggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgc
cgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagctt
aataacactgatagtgctagtgtagatcac