BBa_K1073030
type: ComponentDefinition
persistent identity: BBa K1073030
display id: BBa_K1073030
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible ampicillin resistance cassette + LasR expression cassette
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: The parts for this construct were taken from the 2013 distribution kit.
owned by: james
owned by: myers
mutable description: In complex with the inducer PAI-1 (N-(3-oxododecanoyl)-homoserine lactone), the activator LasR binds to the Las promoter and enables expression. This construct provides all the parts required to express the gene behind the Las promoter. Only the inducer N-(3-oxododecanoyl)-homoserine lactone is missing, which usually is taken up from the media. By the regulation of the inducer's concentration in the media, the expression rate of the gene of interest (in this case ampicillin beta-lactamase) can be controlled.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1073016
component: BBa_B0015
component: BBa_K1073013
sequence annotation: BBa_K1073013
sequence annotation: BBa_B0015
sequence annotation: BBa_K1073016
sequence: https://synbiohub.org/public/igem/BBa_K1073030_sequence/1

BBa_K1073016
type: Component
persistent identity: component2374525
display id: component2374525
version: 1
top level: BBa_K1073030
definition: BBa_K1073016
access: public

BBa_B0015
type: Component
persistent identity: component2374514
display id: component2374514
version: 1
top level: BBa_K1073030
definition: BBa_B0015
access: public

BBa_K1073013
type: Component
persistent identity: component2374507
display id: component2374507
version: 1
top level: BBa_K1073030
definition: BBa_K1073013
access: public

BBa_K1073013
type: SequenceAnnotation
persistent identity: annotation2374507
display id: annotation2374507
version: 1
top level: BBa_K1073030
location: https://synbiohub.org/public/igem/BBa_K1073030/annotation2374507/range2374507/1
component: BBa_K1073013

https://synbiohub.org/public/igem/BBa_K1073030/annotation2374507/range2374507/1
type: Range
persistent identity: range2374507
display id: range2374507
version: 1
top level: BBa_K1073030
direction: direction/reverse
start: 1
end: 1017
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2374514
display id: annotation2374514
version: 1
top level: BBa_K1073030
location: https://synbiohub.org/public/igem/BBa_K1073030/annotation2374514/range2374514/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1073030/annotation2374514/range2374514/1
type: Range
persistent identity: range2374514
display id: range2374514
version: 1
top level: BBa_K1073030
direction: direction/reverse
start: 1026
end: 1154
orientation: inline

BBa_K1073016
type: SequenceAnnotation
persistent identity: annotation2374525
display id: annotation2374525
version: 1
top level: BBa_K1073030
location: https://synbiohub.org/public/igem/BBa_K1073030/annotation2374525/range2374525/1
component: BBa_K1073016

https://synbiohub.org/public/igem/BBa_K1073030/annotation2374525/range2374525/1
type: Range
persistent identity: range2374525
display id: range2374525
version: 1
top level: BBa_K1073030
direction: direction/reverse
start: 1163
end: 2005
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1073013
type: ComponentDefinition
persistent identity: BBa K1073013
display id: BBa_K1073013
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible ampicillin resistance cassette (regulated by LasR + N-3-oxododecanoyl-HSL)
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: This part is a combination of BBa_K1073003 and BBa_K1073000, which themselves were constructed from parts of the 2013 distribution kit.
owned by: james
owned by: myers
mutable description: The promoter for this beta-lactonase is induced by N-(3-oxododecanoyl)-homoserine lactone. Therefore the expression of the ampicillin beta-lactonase is inducable by Las inducer N-(3-oxododecanoyl)-homoserine lactone.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1073000
component: BBa_K1073003
sequence annotation: BBa_K1073003
sequence annotation: BBa_K1073000
sequence: https://synbiohub.org/public/igem/BBa_K1073013_sequence/1

BBa_K1073000
type: Component
persistent identity: component2373124
display id: component2373124
version: 1
top level: BBa_K1073013
definition: ampR
access: public

BBa_K1073003
type: Component
persistent identity: component2373123
display id: component2373123
version: 1
top level: BBa_K1073013
definition: pLas + RBS
access: public

BBa_K1073003
type: SequenceAnnotation
persistent identity: annotation2373123
display id: annotation2373123
version: 1
top level: BBa_K1073013
location: https://synbiohub.org/public/igem/BBa_K1073013/annotation2373123/range2373123/1
component: BBa_K1073003

https://synbiohub.org/public/igem/BBa_K1073013/annotation2373123/range2373123/1
type: Range
persistent identity: range2373123
display id: range2373123
version: 1
top level: BBa_K1073013
direction: direction/reverse
start: 1
end: 147
orientation: inline

BBa_K1073000
type: SequenceAnnotation
persistent identity: annotation2373124
display id: annotation2373124
version: 1
top level: BBa_K1073013
location: https://synbiohub.org/public/igem/BBa_K1073013/annotation2373124/range2373124/1
component: BBa_K1073000

https://synbiohub.org/public/igem/BBa_K1073013/annotation2373124/range2373124/1
type: Range
persistent identity: range2373124
display id: range2373124
version: 1
top level: BBa_K1073013
direction: direction/reverse
start: 154
end: 1017
orientation: inline

BBa_K1073016
type: ComponentDefinition
persistent identity: BBa K1073016
display id: BBa_K1073016
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutively expressed transcription regulator LasR + LVA
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: This part is a combination of BBa_K1073006 and BBa_C0079, which are derived from Biobricks of the 2013 distribution kit.
owned by: james
owned by: myers
mutable description: The LasR activator is constitutively expressed by a strong promoter. It accepts the chemical signal AI-1 (made by lasI protein)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K1073006
component: BBa_C0079
sequence annotation: BBa_K1073006
sequence annotation: BBa_C0079
sequence: https://synbiohub.org/public/igem/BBa_K1073016_sequence/1

BBa_K1073006
type: Component
persistent identity: component2354046
display id: component2354046
version: 1
top level: BBa_K1073016
definition: BBa_K1073006
access: public

BBa_C0079
type: Component
persistent identity: component2354051
display id: component2354051
version: 1
top level: BBa_K1073016
definition: lasr
access: public

BBa_K1073006
type: SequenceAnnotation
persistent identity: annotation2354046
display id: annotation2354046
version: 1
top level: BBa_K1073016
location: https://synbiohub.org/public/igem/BBa_K1073016/annotation2354046/range2354046/1
component: BBa_K1073006

https://synbiohub.org/public/igem/BBa_K1073016/annotation2354046/range2354046/1
type: Range
persistent identity: range2354046
display id: range2354046
version: 1
top level: BBa_K1073016
direction: direction/reverse
start: 1
end: 56
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2354051
display id: annotation2354051
version: 1
top level: BBa_K1073016
location: https://synbiohub.org/public/igem/BBa_K1073016/annotation2354051/range2354051/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K1073016/annotation2354051/range2354051/1
type: Range
persistent identity: range2354051
display id: range2354051
version: 1
top level: BBa_K1073016
direction: direction/reverse
start: 63
end: 843
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lasr
type: ComponentDefinition
persistent identity: BBa C0079
display id: BBa_C0079
version: 1
was generated by: iGEM to SBOL conversion
description: lasR activator from P. aeruginosa PAO1(+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: Genbank: NC_002516 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for lasR protein, which accepts chemical signal AI-1 (made by lasI protein)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Mutated 480 from g to a to remove PstI site
star: true
creator: Alvin Carter Powers (Fighting Darwins)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lasR
sequence annotation: G
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0079_sequence/1

lasR
type: SequenceAnnotation
persistent identity: annotation307935
display id: annotation307935
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1
type: Range
persistent identity: range307935
display id: range307935
version: 1
top level: lasr
direction: direction/forward
start: 1
end: 717
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation308006
display id: annotation308006
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1
type: Range
persistent identity: range308006
display id: range308006
version: 1
top level: lasr
direction: direction/forward
start: 480
end: 480
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation307948
display id: annotation307948
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1
type: Range
persistent identity: range307948
display id: range307948
version: 1
top level: lasr
direction: direction/forward
start: 718
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213999
display id: annotation2213999
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1
type: Range
persistent identity: range2213999
display id: range2213999
version: 1
top level: lasr
direction: direction/forward
start: 757
end: 781
orientation: inline

ampR
type: ComponentDefinition
persistent identity: BBa K1073000
display id: BBa_K1073000
version: 1
was generated by: iGEM to SBOL conversion
description: ampicillin resistance
created: 2013-09-15T11:00:00Z
modified: 2016-01-28T01:50:42Z
mutable provenance: The part was derived from BBa_P1002 ampicillin resistance cassette. The sequence for the beta-lactamase was gained by PCR of the original BBa_P1002 with appropiate primers.
owned by: james
owned by: myers
mutable description: This sequence codes for a beta-lactamase, which degrades ampicillin. There is no RBS or other information attached, only the sequence for the enzyme plus prefix and suffix.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 4206
owner id: 15674
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The primer sequences can be found on iGEM Team Braunschweig's wiki.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1073000_sequence/1

pLas + RBS
type: ComponentDefinition
persistent identity: BBa K1073003
display id: BBa_K1073003
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible promoter of the las quorum sensing system with RBS
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: Thos part is a combination of BBa_K091117 and BBa_B0032, which were taken from the 2013 distribution kit.
owned by: james
owned by: myers
mutable description: This construct is derived from BBa_K091117 and BBa_B0032. It combines the las induced promoter with an RBS and can be used to clone it in front of a desired gene of interest, which shall be induced by las.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_K091117
component: BBa_B0032
sequence annotation: BBa_K091117
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K1073003_sequence/1

BBa_K091117
type: Component
persistent identity: component2346228
display id: component2346228
version: 1
top level: pLas + RBS
definition: BBa_K091117
access: public

BBa_B0032
type: Component
persistent identity: component2346230
display id: component2346230
version: 1
top level: pLas + RBS
definition: BBa_B0032
access: public

BBa_K091117
type: SequenceAnnotation
persistent identity: annotation2346228
display id: annotation2346228
version: 1
top level: pLas + RBS
location: https://synbiohub.org/public/igem/BBa_K1073003/annotation2346228/range2346228/1
component: BBa_K091117

https://synbiohub.org/public/igem/BBa_K1073003/annotation2346228/range2346228/1
type: Range
persistent identity: range2346228
display id: range2346228
version: 1
top level: pLas + RBS
direction: direction/reverse
start: 1
end: 126
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2346230
display id: annotation2346230
version: 1
top level: pLas + RBS
location: https://synbiohub.org/public/igem/BBa_K1073003/annotation2346230/range2346230/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1073003/annotation2346230/range2346230/1
type: Range
persistent identity: range2346230
display id: range2346230
version: 1
top level: pLas + RBS
direction: direction/forward
start: 135
end: 147
orientation: inline

BBa_K1073006
type: ComponentDefinition
persistent identity: BBa K1073006
display id: BBa_K1073006
version: 1
was generated by: iGEM to SBOL conversion
description: strong constitutive promoter with RBS
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: This part is a combination of BBa_J23106 and BBa_B0032, which were taken from 2013 distribution kit.
owned by: james
owned by: myers
mutable description: This part combines a constitutive promoter with an RBS and can be cloned in front of a gene of interest. It is a combination of BBa_J23106 and BBa_B0032.
discontinued: false
dominant: false
experience: experience/None
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0032
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K1073006_sequence/1

BBa_B0032
type: Component
persistent identity: component2352910
display id: component2352910
version: 1
top level: BBa_K1073006
definition: BBa_B0032
access: public

BBa_J23100
type: Component
persistent identity: component2352908
display id: component2352908
version: 1
top level: BBa_K1073006
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2352908
display id: annotation2352908
version: 1
top level: BBa_K1073006
location: https://synbiohub.org/public/igem/BBa_K1073006/annotation2352908/range2352908/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1073006/annotation2352908/range2352908/1
type: Range
persistent identity: range2352908
display id: range2352908
version: 1
top level: BBa_K1073006
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2352910
display id: annotation2352910
version: 1
top level: BBa_K1073006
location: https://synbiohub.org/public/igem/BBa_K1073006/annotation2352910/range2352910/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1073006/annotation2352910/range2352910/1
type: Range
persistent identity: range2352910
display id: range2352910
version: 1
top level: BBa_K1073006
direction: direction/forward
start: 44
end: 56
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_K091117
type: ComponentDefinition
persistent identity: BBa K091117
display id: BBa_K091117
version: 1
was generated by: iGEM to SBOL conversion
description: pLas promoter
created: 2008-06-25T11:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: The sequence of the LasI upstream regulatory region is available at http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=176640
owned by: james
owned by: myers
mutable description: This pLas' promoter contains the regulatory region upstream of the LasI gene in Pseudomonas aeruginosa. K091117 includes a LasR binding site as well as a -10 region modified from CATAAA to TATAAA to reflect our pLasXOR promoter design. Activation is expected to occur in the presence of the autoinducer PAI-1 and the LasR protein. The pLas' promoter serves as a control for the evaluation of the pLasXOR promoter, part BBa_K091118.
discontinued: false
dominant: true
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: To minimize leaky transcription, a secondary transcriptional start site was excluded from the design.
star: false
creator: Erin Feeney
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: LasR Binding Site
sequence annotation: -35 Region
sequence annotation: -10 Region
sequence annotation: Primary Transcriptional Start
sequence: https://synbiohub.org/public/igem/BBa_K091117_sequence/1

LasR Binding Site
type: SequenceAnnotation
persistent identity: annotation1964073
display id: annotation1964073
version: 1
top level: BBa_K091117
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964073/range1964073/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K091117/annotation1964073/range1964073/1
type: Range
persistent identity: range1964073
display id: range1964073
version: 1
top level: BBa_K091117
direction: direction/forward
start: 75
end: 93
orientation: inline

-35 Region
type: SequenceAnnotation
persistent identity: annotation1964072
display id: annotation1964072
version: 1
top level: BBa_K091117
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964072/range1964072/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K091117/annotation1964072/range1964072/1
type: Range
persistent identity: range1964072
display id: range1964072
version: 1
top level: BBa_K091117
direction: direction/forward
start: 86
end: 91
orientation: inline

-10 Region
type: SequenceAnnotation
persistent identity: annotation1964074
display id: annotation1964074
version: 1
top level: BBa_K091117
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964074/range1964074/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K091117/annotation1964074/range1964074/1
type: Range
persistent identity: range1964074
display id: range1964074
version: 1
top level: BBa_K091117
direction: direction/forward
start: 113
end: 118
orientation: inline

Primary Transcriptional Start
type: SequenceAnnotation
persistent identity: annotation1964075
display id: annotation1964075
version: 1
top level: BBa_K091117
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964075/range1964075/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K091117/annotation1964075/range1964075/1
type: Range
persistent identity: range1964075
display id: range1964075
version: 1
top level: BBa_K091117
direction: direction/reverse
start: 125
end: 125
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_K1073030_sequence/1
type: Sequence
persistent identity: BBa K1073030 sequence
display id: BBa_K1073030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagttataaaattatgaaatttgtataaattcttcagtactagagtcacacaggaaagtactagatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073013_sequence/1
type: Sequence
persistent identity: BBa K1073013 sequence
display id: BBa_K1073013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagttataaaattatgaaatttgtataaattcttcagtactagagtcacacaggaaagtactagatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073016_sequence/1
type: Sequence
persistent identity: BBa K1073016 sequence
display id: BBa_K1073016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0079_sequence/1
type: Sequence
persistent identity: BBa C0079 sequence
display id: BBa_C0079_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073000_sequence/1
type: Sequence
persistent identity: BBa K1073000 sequence
display id: BBa_K1073000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073003_sequence/1
type: Sequence
persistent identity: BBa K1073003 sequence
display id: BBa_K1073003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagttataaaattatgaaatttgtataaattcttcagtactagagtcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073006_sequence/1
type: Sequence
persistent identity: BBa K1073006 sequence
display id: BBa_K1073006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091117_sequence/1
type: Sequence
persistent identity: BBa K091117 sequence
display id: BBa_K091117_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagttataaaattatgaaatttgtataaattcttcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z