LOCUS       BBa_K1073031            1705 bp    DNA     linear       26-Dec-2024
DEFINITION  Autoinducer synthase LuxI + LVA with combined amilGFP 
            chromoprotein expression cassette
ACCESSION   BBa_K1073031
VERSION     BBa_K1073031.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    599..630
                     /label=LVA
     CDS             20..598
                     /label=luxI
     misc_feature    20..637
                     /label=BBa_C0061
     misc_feature    638..662
                     /label=Help:Barcodes
     CDS             20..637
                     /label=BBa_C0061
     stem_loop       682..725
                     /label=stem_loop
     misc_feature    671..750
                     /label=BBa_B0010
     terminator      671..750
                     /label=BBa_B0010
     stem_loop       766..785
                     /label=T7 TE
     misc_feature    792..792
                     /label=stop
     polyA_site      786..799
                     /label=polya
     misc_feature    759..799
                     /label=BBa_B0012
     terminator      759..799
                     /label=BBa_B0012
     terminator      671..799
                     /label=BBa_B0015
     misc_feature    1..799
                     /label=BBa_K1073017
     promoter        808..842
                     /label=BBa_J23100
     RBS             851..863
                     /label=RBS-3\Weak
     RBS             857..860
                     /label=RBS
     misc_feature    851..863
                     /label=BBa_B0032
     RBS             851..863
                     /label=BBa_B0032
     promoter        808..863
                     /label=BBa_K1073006
     CDS             870..1565
                     /label=amilGFP
     CDS             870..1568
                     /label=BBa_K592010
     misc_feature    808..1568
                     /label=BBa_K1073024
     stem_loop       1588..1631
                     /label=stem_loop
     misc_feature    1577..1656
                     /label=BBa_B0010
     terminator      1577..1656
                     /label=BBa_B0010
     stem_loop       1672..1691
                     /label=T7 TE
     misc_feature    1698..1698
                     /label=stop
     polyA_site      1692..1705
                     /label=polya
     misc_feature    1665..1705
                     /label=BBa_B0012
     terminator      1665..1705
                     /label=BBa_B0012
     terminator      1577..1705
                     /label=BBa_B0015
ORIGIN
        1 tcacacagga aagtactaga tgactataat gataaaaaaa tcggattttt tggcaattcc
       61 atcggaggag tataaaggta ttctaagtct tcgttatcaa gtgtttaagc aaagacttga
      121 gtgggactta gttgtagaaa ataaccttga atcagatgag tatgataact caaatgcaga
      181 atatatttat gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt tattacctac
      241 aacaggtgat tatatgctga aaagtgtttt tcctgaattg cttggtcaac agagtgctcc
      301 caaagatcct aatatagtcg aattaagtcg ttttgctgta ggtaaaaata gctcaaagat
      361 aaataactct gctagtgaaa ttacaatgaa actatttgaa gctatatata aacacgctgt
      421 tagtcaaggt attacagaat atgtaacagt aacatcaaca gcaatagagc gatttttaaa
      481 gcgtattaaa gttccttgtc atcgtattgg agacaaagaa attcatgtat taggtgatac
      541 taaatcggtt gtattgtcta tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc
      601 tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc
      661 tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      721 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      781 gggcctttct gcgtttatat actagagttg acggctagct cagtcctagg tacagtgcta
      841 gctactagag tcacacagga aagtactaga tgtcttattc aaagcatggc atcgtacaag
      901 aaatgaagac gaaataccat atggaaggca gtgtcaatgg ccatgaattt acgatcgaag
      961 gtgtaggaac tgggtaccct tacgaaggga aacagatgtc cgaattagtg atcatcaagc
     1021 ctgcgggaaa accccttcca ttctcctttg acatactgtc atcagtcttt caatatggaa
     1081 accgttgctt cacaaagtac ccggcagaca tgcctgacta tttcaagcaa gcattcccag
     1141 atggaatgtc atatgaaagg tcatttctat ttgaggatgg agcagttgct acagccagct
     1201 ggaacattcg tctcgaagga aattgcttca tccacaaatc catctttcat ggcgtaaact
     1261 ttcccgctga tggacccgta atgaaaaaga agacaattga ctgggataag tccttcgaaa
     1321 aaatgactgt gtctaaagag gtgctaagag gtgacgtgac tatgtttctt atgctcgaag
     1381 gaggtggttc tcacagatgc caatttcact ccacttacaa aacagagaag ccggtcacac
     1441 tgcccccgaa tcatgtcgta gaacatcaaa ttgtgaggac cgaccttggc caaagtgcaa
     1501 aaggctttac agtcaagctg gaagcacatg ccgcggctca tgttaaccct ttgaaggtta
     1561 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1621 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1681 tcgggtgggc ctttctgcgt ttata
//