##gff-version 3
##sequence-region BBa_K1073031 1 1705
BBa_K1073031	.	engineered_region	808	1568	.	+	0	ID=BBa_K1073024;Name=BBa_K1073024
BBa_K1073031	.	promoter	808	863	.	+	0	ID=BBa_K1073006;Name=BBa_K1073006;Parent=BBa_K1073024
BBa_K1073031	.	ribosome_entry_site	851	863	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1073006
BBa_K1073031	.	ribosome_entry_site	857	860	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1073031	.	engineered_region	851	863	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1073031	.	ribosome_entry_site	851	863	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1073031	.	promoter	808	842	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1073006
BBa_K1073031	.	CDS	870	1568	.	+	0	ID=BBa_K592010;Name=BBa_K592010;Parent=BBa_K1073024
BBa_K1073031	.	CDS	870	1565	.	+	0	ID=annotation2131633;Name=amilGFP;Parent=BBa_K592010
BBa_K1073031	.	terminator	1577	1705	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1073031	.	terminator	1577	1656	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1073031	.	stem_loop	1588	1631	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1073031	.	engineered_region	1577	1656	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1073031	.	terminator	1665	1705	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1073031	.	engineered_region	1665	1705	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1073031	.	stop_codon	1698	1698	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1073031	.	stem_loop	1672	1691	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1073031	.	polyA_site	1692	1705	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1073031	.	engineered_region	1	799	.	+	0	ID=BBa_K1073017;Name=BBa_K1073017
BBa_K1073031	.	terminator	671	799	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1073017
BBa_K1073031	.	terminator	671	750	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1073031	.	stem_loop	682	725	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1073031	.	engineered_region	671	750	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1073031	.	terminator	759	799	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1073031	.	engineered_region	759	799	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1073031	.	stop_codon	792	792	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1073031	.	stem_loop	766	785	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1073031	.	polyA_site	786	799	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1073031	.	CDS	20	637	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K1073017
BBa_K1073031	.	engineered_region	20	637	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1073031	.	tag	599	630	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1073031	.	engineered_tag	638	662	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1073031	.	CDS	20	598	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1073031	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1073017
BBa_K1073031	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1073031	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1073031	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
>BBa_K1073031
tcacacaggaaagtactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggt
attctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatg
agtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacc
tacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatata
gtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactat
ttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatt
tttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgta
ttgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagctt
aataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactag
atgtcttattcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaat
ttacgatcgaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcggg
aaaaccccttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggca
gacatgcctgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagcag
ttgctacagccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttcc
cgctgatggacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggtg
ctaagaggtgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaaa
cagagaagccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaaa
aggctttacagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggttaaataataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttata