Autoinducer synthase LuxI + LVA with combined amilGFP chromoprotein expression cassette
The biobricks were taken from the 2013 distribution kit and the iGEM Team Uppsala.
This part is the basis for the expression of the Lux auto inducer synthetase, which synthesizes LuxI [N-(3-oxohexanoyl)-homoserine lactone]. LuxI binds to the activator LuxR. Together they activate the Lux promoter. A constitutive expression of amil GFP is added to the construct in order to varify the expression of LuxI synthetase. The construct has no promoter in front of the LuxI synthetase gene. Therefore the user can choose the promoter himself (e.g. constitutive, induced, repressed, etc.)
Double terminator consisting of BBa_B0010 and BBa_B0012
Lux I + LVA with RBS and double terminator
This part is a combination of BBa_B0032 (RBS), BBa_C0061 (synthetase) and BBa_B0015 (TT), which were taken from the 2013 distribution kit.
To the autoinducer synthetase for AHL (BBa_C0061) a RBS and a double terminator was added to facilitate the construction of an expression system. The enzyme synthesizes 3OC6HSL, which binds to LuxR.
Constitutively expressed chromoprotein amilGFP
This part is a combination of BBa_K1073006 and BBa_K592010, which were derived from the 2013 distribution kit and the iGEM Team Uppsala.
The chromoprotein amilGFP is constitutively expressed and can be used for the marking of cells for easier differentiation.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
RBS.3 (medium) -- derivative of BBa_0030
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
V. fischeri <genbank>AF170104</genbank>
Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
Transcriptional terminator consisting of a 64 bp stem-loop.
This part is a combination of BBa_J23106 and BBa_B0032, which were taken from 2013 distribution kit.
This part combines a constitutive promoter with an RBS and can be cloned in front of a gene of interest. It is a combination of BBa_J23106 and BBa_B0032.
This chromoprotein, amilGFP, naturally exhibits very strong yellow color when expressed. The color is strong and readily visible to naked eye both in LB-culture and on agar plates. The DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of two illegal internal restriction sites (EcoRI and PstI).
Two illegal internal restriction sites (EcoRI and PstI) was removed.
Conversion of the iGEM parts registry to SBOL2.1