BBa_K1073031
type: ComponentDefinition
persistent identity: BBa K1073031
display id: BBa_K1073031
version: 1
was generated by: iGEM to SBOL conversion
description: Autoinducer synthase LuxI + LVA with combined amilGFP chromoprotein expression cassette
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: The biobricks were taken from the 2013 distribution kit and the iGEM Team Uppsala.
owned by: james
owned by: myers
mutable description: This part is the basis for the expression of the Lux auto inducer synthetase, which synthesizes LuxI [N-(3-oxohexanoyl)-homoserine lactone]. LuxI binds to the activator LuxR. Together they activate the Lux promoter. A constitutive expression of amil GFP is added to the construct in order to varify the expression of LuxI synthetase. The construct has no promoter in front of the LuxI synthetase gene. Therefore the user can choose the promoter himself (e.g. constitutive, induced, repressed, etc.)
discontinued: false
dominant: false
experience: experience/None
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K1073024
component: BBa_K1073017
sequence annotation: BBa_K1073017
sequence annotation: BBa_K1073024
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1073031_sequence/1

BBa_B0015
type: Component
persistent identity: component2376894
display id: component2376894
version: 1
top level: BBa_K1073031
definition: BBa_B0015
access: public

BBa_K1073024
type: Component
persistent identity: component2376887
display id: component2376887
version: 1
top level: BBa_K1073031
definition: BBa_K1073024
access: public

BBa_K1073017
type: Component
persistent identity: component2376879
display id: component2376879
version: 1
top level: BBa_K1073031
definition: BBa_K1073017
access: public

BBa_K1073017
type: SequenceAnnotation
persistent identity: annotation2376879
display id: annotation2376879
version: 1
top level: BBa_K1073031
location: https://synbiohub.org/public/igem/BBa_K1073031/annotation2376879/range2376879/1
component: BBa_K1073017

https://synbiohub.org/public/igem/BBa_K1073031/annotation2376879/range2376879/1
type: Range
persistent identity: range2376879
display id: range2376879
version: 1
top level: BBa_K1073031
direction: direction/reverse
start: 1
end: 799
orientation: inline

BBa_K1073024
type: SequenceAnnotation
persistent identity: annotation2376887
display id: annotation2376887
version: 1
top level: BBa_K1073031
location: https://synbiohub.org/public/igem/BBa_K1073031/annotation2376887/range2376887/1
component: BBa_K1073024

https://synbiohub.org/public/igem/BBa_K1073031/annotation2376887/range2376887/1
type: Range
persistent identity: range2376887
display id: range2376887
version: 1
top level: BBa_K1073031
direction: direction/reverse
start: 808
end: 1568
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2376894
display id: annotation2376894
version: 1
top level: BBa_K1073031
location: https://synbiohub.org/public/igem/BBa_K1073031/annotation2376894/range2376894/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1073031/annotation2376894/range2376894/1
type: Range
persistent identity: range2376894
display id: range2376894
version: 1
top level: BBa_K1073031
direction: direction/reverse
start: 1577
end: 1705
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1073017
type: ComponentDefinition
persistent identity: BBa K1073017
display id: BBa_K1073017
version: 1
was generated by: iGEM to SBOL conversion
description: Lux I + LVA with RBS and double terminator
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: This part is a combination of BBa_B0032 (RBS), BBa_C0061 (synthetase) and BBa_B0015 (TT), which were taken from the 2013 distribution kit.
owned by: james
owned by: myers
mutable description: To the autoinducer synthetase for AHL (BBa_C0061) a RBS and a double terminator was added to facilitate the construction of an expression system. The enzyme synthesizes 3OC6HSL, which binds to LuxR.
discontinued: false
dominant: false
experience: experience/None
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0032
component: BBa_B0015
component: BBa_C0061
sequence annotation: BBa_B0032
sequence annotation: BBa_C0061
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1073017_sequence/1

BBa_B0032
type: Component
persistent identity: component2349309
display id: component2349309
version: 1
top level: BBa_K1073017
definition: BBa_B0032
access: public

BBa_B0015
type: Component
persistent identity: component2349321
display id: component2349321
version: 1
top level: BBa_K1073017
definition: BBa_B0015
access: public

BBa_C0061
type: Component
persistent identity: component2349312
display id: component2349312
version: 1
top level: BBa_K1073017
definition: luxI
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2349309
display id: annotation2349309
version: 1
top level: BBa_K1073017
location: https://synbiohub.org/public/igem/BBa_K1073017/annotation2349309/range2349309/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1073017/annotation2349309/range2349309/1
type: Range
persistent identity: range2349309
display id: range2349309
version: 1
top level: BBa_K1073017
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2349312
display id: annotation2349312
version: 1
top level: BBa_K1073017
location: https://synbiohub.org/public/igem/BBa_K1073017/annotation2349312/range2349312/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K1073017/annotation2349312/range2349312/1
type: Range
persistent identity: range2349312
display id: range2349312
version: 1
top level: BBa_K1073017
direction: direction/forward
start: 20
end: 637
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2349321
display id: annotation2349321
version: 1
top level: BBa_K1073017
location: https://synbiohub.org/public/igem/BBa_K1073017/annotation2349321/range2349321/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1073017/annotation2349321/range2349321/1
type: Range
persistent identity: range2349321
display id: range2349321
version: 1
top level: BBa_K1073017
direction: direction/reverse
start: 671
end: 799
orientation: inline

BBa_K1073024
type: ComponentDefinition
persistent identity: BBa K1073024
display id: BBa_K1073024
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutively expressed chromoprotein amilGFP
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: This part is a combination of BBa_K1073006 and BBa_K592010, which were derived from the 2013 distribution kit and the iGEM Team Uppsala.
owned by: james
owned by: myers
mutable description: The chromoprotein amilGFP is constitutively expressed and can be used for the marking of cells for easier differentiation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592010
component: BBa_K1073006
sequence annotation: BBa_K1073006
sequence annotation: BBa_K592010
sequence: https://synbiohub.org/public/igem/BBa_K1073024_sequence/1

BBa_K592010
type: Component
persistent identity: component2354086
display id: component2354086
version: 1
top level: BBa_K1073024
definition: amilGFP
access: public

BBa_K1073006
type: Component
persistent identity: component2354084
display id: component2354084
version: 1
top level: BBa_K1073024
definition: BBa_K1073006
access: public

BBa_K1073006
type: SequenceAnnotation
persistent identity: annotation2354084
display id: annotation2354084
version: 1
top level: BBa_K1073024
location: https://synbiohub.org/public/igem/BBa_K1073024/annotation2354084/range2354084/1
component: BBa_K1073006

https://synbiohub.org/public/igem/BBa_K1073024/annotation2354084/range2354084/1
type: Range
persistent identity: range2354084
display id: range2354084
version: 1
top level: BBa_K1073024
direction: direction/reverse
start: 1
end: 56
orientation: inline

BBa_K592010
type: SequenceAnnotation
persistent identity: annotation2354086
display id: annotation2354086
version: 1
top level: BBa_K1073024
location: https://synbiohub.org/public/igem/BBa_K1073024/annotation2354086/range2354086/1
component: BBa_K592010

https://synbiohub.org/public/igem/BBa_K1073024/annotation2354086/range2354086/1
type: Range
persistent identity: range2354086
display id: range2354086
version: 1
top level: BBa_K1073024
direction: direction/reverse
start: 63
end: 761
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K1073006
type: ComponentDefinition
persistent identity: BBa K1073006
display id: BBa_K1073006
version: 1
was generated by: iGEM to SBOL conversion
description: strong constitutive promoter with RBS
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: This part is a combination of BBa_J23106 and BBa_B0032, which were taken from 2013 distribution kit.
owned by: james
owned by: myers
mutable description: This part combines a constitutive promoter with an RBS and can be cloned in front of a gene of interest. It is a combination of BBa_J23106 and BBa_B0032.
discontinued: false
dominant: false
experience: experience/None
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0032
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K1073006_sequence/1

BBa_B0032
type: Component
persistent identity: component2352910
display id: component2352910
version: 1
top level: BBa_K1073006
definition: BBa_B0032
access: public

BBa_J23100
type: Component
persistent identity: component2352908
display id: component2352908
version: 1
top level: BBa_K1073006
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2352908
display id: annotation2352908
version: 1
top level: BBa_K1073006
location: https://synbiohub.org/public/igem/BBa_K1073006/annotation2352908/range2352908/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1073006/annotation2352908/range2352908/1
type: Range
persistent identity: range2352908
display id: range2352908
version: 1
top level: BBa_K1073006
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2352910
display id: annotation2352910
version: 1
top level: BBa_K1073006
location: https://synbiohub.org/public/igem/BBa_K1073006/annotation2352910/range2352910/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1073006/annotation2352910/range2352910/1
type: Range
persistent identity: range2352910
display id: range2352910
version: 1
top level: BBa_K1073006
direction: direction/forward
start: 44
end: 56
orientation: inline

amilGFP
type: ComponentDefinition
persistent identity: BBa K592010
display id: BBa_K592010
version: 1
was generated by: iGEM to SBOL conversion
description: amilGFP, yellow chromoprotein
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: Acropora millepora
owned by: james
owned by: myers
mutable description: This chromoprotein, amilGFP, naturally exhibits very strong yellow color when expressed. The color is strong and readily visible to naked eye both in LB-culture and on agar plates. The DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of two illegal internal restriction sites (EcoRI and PstI).
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Two illegal internal restriction sites (EcoRI and PstI) was removed.
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: amilGFP
sequence: https://synbiohub.org/public/igem/BBa_K592010_sequence/1

amilGFP
type: SequenceAnnotation
persistent identity: annotation2131633
display id: annotation2131633
version: 1
top level: amilGFP
location: https://synbiohub.org/public/igem/BBa_K592010/annotation2131633/range2131633/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K592010/annotation2131633/range2131633/1
type: Range
persistent identity: range2131633
display id: range2131633
version: 1
top level: amilGFP
direction: direction/forward
start: 1
end: 696
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_K1073031_sequence/1
type: Sequence
persistent identity: BBa K1073031 sequence
display id: BBa_K1073031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatgtcttattcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaatttacgatcgaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcgggaaaaccccttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggcagacatgcctgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagcagttgctacagccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttcccgctgatggacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggtgctaagaggtgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaaacagagaagccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaaaaggctttacagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggttaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073017_sequence/1
type: Sequence
persistent identity: BBa K1073017 sequence
display id: BBa_K1073017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073024_sequence/1
type: Sequence
persistent identity: BBa K1073024 sequence
display id: BBa_K1073024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatgtcttattcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaatttacgatcgaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcgggaaaaccccttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggcagacatgcctgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagcagttgctacagccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttcccgctgatggacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggtgctaagaggtgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaaacagagaagccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaaaaggctttacagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggttaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073006_sequence/1
type: Sequence
persistent identity: BBa K1073006 sequence
display id: BBa_K1073006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592010_sequence/1
type: Sequence
persistent identity: BBa K592010 sequence
display id: BBa_K592010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcttattcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaatttacgatcgaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcgggaaaaccccttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggcagacatgcctgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagcagttgctacagccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttcccgctgatggacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggtgctaagaggtgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaaacagagaagccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaaaaggctttacagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggttaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z