BBa_K1073032
type: ComponentDefinition
persistent identity: BBa K1073032
display id: BBa_K1073032
version: 1
was generated by: iGEM to SBOL conversion
description: Autoinducer synthase LasI + LVA with combined eforRed chromoprotein expression cassette
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: The biobricks for this part were derived from the 2013 distribution kit and the iGEM Team Uppsala.
owned by: james
owned by: myers
mutable description: This part is the basis for the expression of the Las auto inducer synthetase, which synthesizes LasI [N-(3-oxododecanoyl)-homoserine lactone]. LasI binds to the activator LasR. Together they activate the Las promoter. A constitutive expression of the chromoprotein eforRed is added to the construct in order to varify the expression of LasI synthetase. The construct has no promoter in front of the LasI synthetase gene. Therefore the user can choose the promoter himself (e.g. constitutive, induced, repressed, etc.)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K1073022
component: BBa_K1073018
sequence annotation: BBa_K1073018
sequence annotation: BBa_K1073022
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1073032_sequence/1

BBa_B0015
type: Component
persistent identity: component2354180
display id: component2354180
version: 1
top level: BBa_K1073032
definition: BBa_B0015
access: public

BBa_K1073022
type: Component
persistent identity: component2354173
display id: component2354173
version: 1
top level: BBa_K1073032
definition: BBa_K1073022
access: public

BBa_K1073018
type: Component
persistent identity: component2354165
display id: component2354165
version: 1
top level: BBa_K1073032
definition: BBa_K1073018
access: public

BBa_K1073018
type: SequenceAnnotation
persistent identity: annotation2354165
display id: annotation2354165
version: 1
top level: BBa_K1073032
location: https://synbiohub.org/public/igem/BBa_K1073032/annotation2354165/range2354165/1
component: BBa_K1073018

https://synbiohub.org/public/igem/BBa_K1073032/annotation2354165/range2354165/1
type: Range
persistent identity: range2354165
display id: range2354165
version: 1
top level: BBa_K1073032
direction: direction/reverse
start: 1
end: 823
orientation: inline

BBa_K1073022
type: SequenceAnnotation
persistent identity: annotation2354173
display id: annotation2354173
version: 1
top level: BBa_K1073032
location: https://synbiohub.org/public/igem/BBa_K1073032/annotation2354173/range2354173/1
component: BBa_K1073022

https://synbiohub.org/public/igem/BBa_K1073032/annotation2354173/range2354173/1
type: Range
persistent identity: range2354173
display id: range2354173
version: 1
top level: BBa_K1073032
direction: direction/reverse
start: 832
end: 1574
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2354180
display id: annotation2354180
version: 1
top level: BBa_K1073032
location: https://synbiohub.org/public/igem/BBa_K1073032/annotation2354180/range2354180/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1073032/annotation2354180/range2354180/1
type: Range
persistent identity: range2354180
display id: range2354180
version: 1
top level: BBa_K1073032
direction: direction/reverse
start: 1583
end: 1711
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1073018
type: ComponentDefinition
persistent identity: BBa K1073018
display id: BBa_K1073018
version: 1
was generated by: iGEM to SBOL conversion
description: Autoinducer synthetase LasI ( producing N-3-oxododecanoyl-HSL) with RBS and double terminator
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: The part is a combination of BBa_B0032, BBa_C0078, and BBa_B0015, which were taken from the 2013 distribution kit.
owned by: james
owned by: myers
mutable description: To the lasI protein, which produces the chemical signal AI-1, a RBS and a double terminator was added to facilitate the construction of an expression system.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0078
component: BBa_B0032
component: BBa_B0015
sequence annotation: BBa_B0032
sequence annotation: BBa_C0078
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1073018_sequence/1

BBa_C0078
type: Component
persistent identity: component2354058
display id: component2354058
version: 1
top level: BBa_K1073018
definition: lasI
access: public

BBa_B0032
type: Component
persistent identity: component2354053
display id: component2354053
version: 1
top level: BBa_K1073018
definition: BBa_B0032
access: public

BBa_B0015
type: Component
persistent identity: component2354065
display id: component2354065
version: 1
top level: BBa_K1073018
definition: BBa_B0015
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2354053
display id: annotation2354053
version: 1
top level: BBa_K1073018
location: https://synbiohub.org/public/igem/BBa_K1073018/annotation2354053/range2354053/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1073018/annotation2354053/range2354053/1
type: Range
persistent identity: range2354053
display id: range2354053
version: 1
top level: BBa_K1073018
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation2354058
display id: annotation2354058
version: 1
top level: BBa_K1073018
location: https://synbiohub.org/public/igem/BBa_K1073018/annotation2354058/range2354058/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_K1073018/annotation2354058/range2354058/1
type: Range
persistent identity: range2354058
display id: range2354058
version: 1
top level: BBa_K1073018
direction: direction/reverse
start: 20
end: 686
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2354065
display id: annotation2354065
version: 1
top level: BBa_K1073018
location: https://synbiohub.org/public/igem/BBa_K1073018/annotation2354065/range2354065/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1073018/annotation2354065/range2354065/1
type: Range
persistent identity: range2354065
display id: range2354065
version: 1
top level: BBa_K1073018
direction: direction/reverse
start: 695
end: 823
orientation: inline

BBa_K1073022
type: ComponentDefinition
persistent identity: BBa K1073022
display id: BBa_K1073022
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutively expressed chromoprotein eforRed
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: This part is a combination of BBa_K1073006 and BBa_K592012, which were derived from the 2013 distribution kit and the iGEM Team Uppsala.
owned by: james
owned by: myers
mutable description: The chromoprotein eforRed is constitutively expressed and can be used for the marking of cells for easier differentiation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592012
component: BBa_K1073006
sequence annotation: BBa_K1073006
sequence annotation: BBa_K592012
sequence: https://synbiohub.org/public/igem/BBa_K1073022_sequence/1

BBa_K592012
type: Component
persistent identity: component2354079
display id: component2354079
version: 1
top level: BBa_K1073022
definition: eforRed
access: public

BBa_K1073006
type: Component
persistent identity: component2354077
display id: component2354077
version: 1
top level: BBa_K1073022
definition: BBa_K1073006
access: public

BBa_K1073006
type: SequenceAnnotation
persistent identity: annotation2354077
display id: annotation2354077
version: 1
top level: BBa_K1073022
location: https://synbiohub.org/public/igem/BBa_K1073022/annotation2354077/range2354077/1
component: BBa_K1073006

https://synbiohub.org/public/igem/BBa_K1073022/annotation2354077/range2354077/1
type: Range
persistent identity: range2354077
display id: range2354077
version: 1
top level: BBa_K1073022
direction: direction/reverse
start: 1
end: 56
orientation: inline

BBa_K592012
type: SequenceAnnotation
persistent identity: annotation2354079
display id: annotation2354079
version: 1
top level: BBa_K1073022
location: https://synbiohub.org/public/igem/BBa_K1073022/annotation2354079/range2354079/1
component: BBa_K592012

https://synbiohub.org/public/igem/BBa_K1073022/annotation2354079/range2354079/1
type: Range
persistent identity: range2354079
display id: range2354079
version: 1
top level: BBa_K1073022
direction: direction/reverse
start: 63
end: 743
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lasI
type: ComponentDefinition
persistent identity: BBa C0078
display id: BBa_C0078
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for PAI from Pseudomonas aeruginosa
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for lasI protein, which produces the chemical signal AI-1
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Chris Walsh (Fighting Darwins)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LasI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0078_sequence/1

LasI
type: SequenceAnnotation
persistent identity: annotation305970
display id: annotation305970
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1
type: Range
persistent identity: range305970
display id: range305970
version: 1
top level: lasI
direction: direction/forward
start: 1
end: 603
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306608
display id: annotation306608
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1
type: Range
persistent identity: range306608
display id: range306608
version: 1
top level: lasI
direction: direction/forward
start: 604
end: 636
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214003
display id: annotation2214003
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1
type: Range
persistent identity: range2214003
display id: range2214003
version: 1
top level: lasI
direction: direction/forward
start: 643
end: 667
orientation: inline

BBa_K1073006
type: ComponentDefinition
persistent identity: BBa K1073006
display id: BBa_K1073006
version: 1
was generated by: iGEM to SBOL conversion
description: strong constitutive promoter with RBS
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: This part is a combination of BBa_J23106 and BBa_B0032, which were taken from 2013 distribution kit.
owned by: james
owned by: myers
mutable description: This part combines a constitutive promoter with an RBS and can be cloned in front of a gene of interest. It is a combination of BBa_J23106 and BBa_B0032.
discontinued: false
dominant: false
experience: experience/None
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0032
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K1073006_sequence/1

BBa_B0032
type: Component
persistent identity: component2352910
display id: component2352910
version: 1
top level: BBa_K1073006
definition: BBa_B0032
access: public

BBa_J23100
type: Component
persistent identity: component2352908
display id: component2352908
version: 1
top level: BBa_K1073006
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2352908
display id: annotation2352908
version: 1
top level: BBa_K1073006
location: https://synbiohub.org/public/igem/BBa_K1073006/annotation2352908/range2352908/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1073006/annotation2352908/range2352908/1
type: Range
persistent identity: range2352908
display id: range2352908
version: 1
top level: BBa_K1073006
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2352910
display id: annotation2352910
version: 1
top level: BBa_K1073006
location: https://synbiohub.org/public/igem/BBa_K1073006/annotation2352910/range2352910/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1073006/annotation2352910/range2352910/1
type: Range
persistent identity: range2352910
display id: range2352910
version: 1
top level: BBa_K1073006
direction: direction/forward
start: 44
end: 56
orientation: inline

eforRed
type: ComponentDefinition
persistent identity: BBa K592012
display id: BBa_K592012
version: 1
was generated by: iGEM to SBOL conversion
description: eforRed, red chromoprotein
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:49Z
mutable provenance: coming soon
owned by: james
owned by: myers
mutable description: This chromoprotein eforRed naturally exhibits red color when expressed. The color is weaker than RFP, however. On agar plates and in liquid culture, the color is readily visible to naked eye in less than 24 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: coming soon
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: eforRed
sequence: https://synbiohub.org/public/igem/BBa_K592012_sequence/1

eforRed
type: SequenceAnnotation
persistent identity: annotation2131799
display id: annotation2131799
version: 1
top level: eforRed
location: https://synbiohub.org/public/igem/BBa_K592012/annotation2131799/range2131799/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K592012/annotation2131799/range2131799/1
type: Range
persistent identity: range2131799
display id: range2131799
version: 1
top level: eforRed
direction: direction/forward
start: 1
end: 681
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_K1073032_sequence/1
type: Sequence
persistent identity: BBa K1073032 sequence
display id: BBa_K1073032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatgtcagtgattaagcaggtaatgaagaccaagttgcaccttgagggcactgtcaatggccatgattttacgatcgagggtaaaggtgaaggcaagccgtacgaagggttacagcacatgaaaatgacagtcaccaaaggcgcgcctctgccgttttccgttcatattcttacacctagccacatgtatggaagcaaaccgtttaataagtatccagcggatatcccagactaccacaaacagtcttttcccgaaggtatgtcttgggagcggtcgatgatttttgaagatggtggcgtatgcaccgccagtaatcactccagcataaacttgcaagagaactgtttcatctatgatgttaaatttcatggtgtgaacctgcctccggatgggcccgtaatgcaaaaaaccattgctggatgggagccgagcgtggaaacactgtacgtgcgtgacgggatgttaaaaagtgacactgcaatggtttttaaactgaaaggaggcggtcatcatcgtgttgatttcaaaacgacgtataaagccaaaaaacctgtcaagctgccagaatttcatttcgttgaacatcgcctggaactgaccaaacacgataaagatttcacaacttgggaccagcaggaggcagccgaaggccatttctcaccgctgccgaaggctctcccatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073018_sequence/1
type: Sequence
persistent identity: BBa K1073018 sequence
display id: BBa_K1073018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073022_sequence/1
type: Sequence
persistent identity: BBa K1073022 sequence
display id: BBa_K1073022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatgtcagtgattaagcaggtaatgaagaccaagttgcaccttgagggcactgtcaatggccatgattttacgatcgagggtaaaggtgaaggcaagccgtacgaagggttacagcacatgaaaatgacagtcaccaaaggcgcgcctctgccgttttccgttcatattcttacacctagccacatgtatggaagcaaaccgtttaataagtatccagcggatatcccagactaccacaaacagtcttttcccgaaggtatgtcttgggagcggtcgatgatttttgaagatggtggcgtatgcaccgccagtaatcactccagcataaacttgcaagagaactgtttcatctatgatgttaaatttcatggtgtgaacctgcctccggatgggcccgtaatgcaaaaaaccattgctggatgggagccgagcgtggaaacactgtacgtgcgtgacgggatgttaaaaagtgacactgcaatggtttttaaactgaaaggaggcggtcatcatcgtgttgatttcaaaacgacgtataaagccaaaaaacctgtcaagctgccagaatttcatttcgttgaacatcgcctggaactgaccaaacacgataaagatttcacaacttgggaccagcaggaggcagccgaaggccatttctcaccgctgccgaaggctctccca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0078_sequence/1
type: Sequence
persistent identity: BBa C0078 sequence
display id: BBa_C0078_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073006_sequence/1
type: Sequence
persistent identity: BBa K1073006 sequence
display id: BBa_K1073006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592012_sequence/1
type: Sequence
persistent identity: BBa K592012 sequence
display id: BBa_K592012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcagtgattaagcaggtaatgaagaccaagttgcaccttgagggcactgtcaatggccatgattttacgatcgagggtaaaggtgaaggcaagccgtacgaagggttacagcacatgaaaatgacagtcaccaaaggcgcgcctctgccgttttccgttcatattcttacacctagccacatgtatggaagcaaaccgtttaataagtatccagcggatatcccagactaccacaaacagtcttttcccgaaggtatgtcttgggagcggtcgatgatttttgaagatggtggcgtatgcaccgccagtaatcactccagcataaacttgcaagagaactgtttcatctatgatgttaaatttcatggtgtgaacctgcctccggatgggcccgtaatgcaaaaaaccattgctggatgggagccgagcgtggaaacactgtacgtgcgtgacgggatgttaaaaagtgacactgcaatggtttttaaactgaaaggaggcggtcatcatcgtgttgatttcaaaacgacgtataaagccaaaaaacctgtcaagctgccagaatttcatttcgttgaacatcgcctggaactgaccaaacacgataaagatttcacaacttgggaccagcaggaggcagccgaaggccatttctcaccgctgccgaaggctctccca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z