LOCUS       BBa_K1073033            1729 bp    DNA     linear       24-Dec-2025
DEFINITION  Autoinducer synthase RhlI + LVA with combined aeBlue chromoprotein 
            expression cassette
ACCESSION   BBa_K1073033
VERSION     BBa_K1073033.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..622
                     /label=rhlL
     misc_feature    623..655
                     /label=LVA
     misc_feature    662..686
                     /label=Help:Barcodes
     CDS             20..686
                     /label=BBa_C0070
     stem_loop       706..749
                     /label=stem_loop
     misc_feature    695..774
                     /label=BBa_B0010
     terminator      695..774
                     /label=BBa_B0010
     stem_loop       790..809
                     /label=T7 TE
     misc_feature    816..816
                     /label=stop
     polyA_site      810..823
                     /label=polya
     misc_feature    783..823
                     /label=BBa_B0012
     terminator      783..823
                     /label=BBa_B0012
     terminator      695..823
                     /label=BBa_B0015
     misc_feature    1..823
                     /label=BBa_K1073019
     promoter        832..866
                     /label=BBa_J23100
     RBS             875..887
                     /label=RBS-3\Weak
     RBS             881..884
                     /label=RBS
     misc_feature    875..887
                     /label=BBa_B0032
     RBS             875..887
                     /label=BBa_B0032
     promoter        832..887
                     /label=BBa_K1073006
     CDS             894..1592
                     /label=aeBlue
     CDS             894..1592
                     /label=BBa_K864401
     misc_feature    832..1592
                     /label=BBa_K1073020
     stem_loop       1612..1655
                     /label=stem_loop
     misc_feature    1601..1680
                     /label=BBa_B0010
     terminator      1601..1680
                     /label=BBa_B0010
     stem_loop       1696..1715
                     /label=T7 TE
     misc_feature    1722..1722
                     /label=stop
     polyA_site      1716..1729
                     /label=polya
     misc_feature    1689..1729
                     /label=BBa_B0012
     terminator      1689..1729
                     /label=BBa_B0012
     terminator      1601..1729
                     /label=BBa_B0015
ORIGIN
        1 tcacacagga aagtactaga tgatcgaact gctgtccgaa tccctggaag gtctgtccgc
       61 tgctatgatc gctgaactgg gtcgttaccg tcaccaggtt ttcatcgaaa aactgggttg
      121 ggacgttgtt tccacctccc gtgttcgtga ccaggagttc gaccagttcg accacccgca
      181 gacccgttac atcgttgcta tgtcccgtca gggtatctgc ggttgcgctc gtctgctgcc
      241 gaccaccgac gcttacctgc tgaaagacgt tttcgcttac ctgtgctccg aaaccccgcc
      301 gtccgacccg tccgtttggg aactgtcccg ttacgctgct tccgctgctg acgacccgca
      361 gctggctatg aaaatcttct ggtcctccct ccagtgcgct tggtacctgg gtgcttcctc
      421 cgttgttgct gttaccacca ccgctatgga acgttacttc gttcgtaacg gtgttatcct
      481 ccagcgtctg ggtccgccgc agaaagttaa aggtgaaacc ctggttgcta tctccttccc
      541 ggcttaccag gaacgtggtc tggaaatgct gctgcgttac cacccggaat ggctccaggg
      601 tgttccgctg tccatggctg ttgctgcaaa cgacgaaaac tacgctttag tagcttaata
      661 actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct
      721 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      781 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gttgacggct
      841 agctcagtcc taggtacagt gctagctact agagtcacac aggaaagtac tagatggctt
      901 cactggttaa aaaagacatg tgcatcaaaa tgacgatgga aggaacagta aacggtcacc
      961 atttcaagtg tgtaggagaa ggcgaaggca aaccatttga agggacccag gtggaaaaga
     1021 tacgcatcac tgaaggtggg cccttaccat ttgcgtatga tattttggcc ccttgttgca
     1081 tgtatggcag taaaaccttc attaagcatg tgtcgggtat tccggattac tttaaggagt
     1141 cttttcctga gggctttacc tgggaaagaa cacaaatctt cgaggatggc ggctatctca
     1201 ccatacacca ggacacgagc cttcagggta ataattttat tttcaaagtt aatgtcatcg
     1261 gtgccaactt ccctgcaaac ggtcccgtga tgcagaaaaa aacagctgga tgggaaccgt
     1321 gcgttgagat gctttatccg cgggacggcg tcctgtgtgg tcagagcctg atggccctga
     1381 aatgcactga tggcaatcat ctgacgtccc acctgcgcac tacctatcgt tctcgcaagc
     1441 catccaatgc agttaacatg ccggaatttc attttgggga tcatcgcatt gagattttga
     1501 aagctgaaca aggtaaattt tatgaacaat acgagtcagc ggtggcccgt tactgtgagg
     1561 cggcaccgag taaattaggg catcactaat aatactagag ccaggcatca aataaaacga
     1621 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1681 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//