BBa_K1073033
type: ComponentDefinition
persistent identity: BBa K1073033
display id: BBa_K1073033
version: 1
was generated by: iGEM to SBOL conversion
description: Autoinducer synthase RhlI + LVA with combined aeBlue chromoprotein expression cassette
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: The biobricks for this part were derived from the 2013 distribution kit and the iGEM Team Uppsala.
owned by: james
owned by: myers
mutable description: This part is the basis for the expression of the Rhl auto inducer synthetase, which synthesizes RhlI [N-(butyryl)-homoserine lactone]. RhlI binds to the activator RhlR. Together they activate the Rhl promoter. A constitutive expression of aeblue is added to the construct in order to varify the expression of RhlI synthetase. The construct has no promoter in front of the RhlI synthetase gene. Therefore the user can choose the promoter himself (e.g. constitutive, induced, repressed, etc.)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_K1073020
component: BBa_K1073019
sequence annotation: BBa_K1073019
sequence annotation: BBa_K1073020
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1073033_sequence/1

BBa_B0015
type: Component
persistent identity: component2354210
display id: component2354210
version: 1
top level: BBa_K1073033
definition: BBa_B0015
access: public

BBa_K1073020
type: Component
persistent identity: component2354203
display id: component2354203
version: 1
top level: BBa_K1073033
definition: aeblue
access: public

BBa_K1073019
type: Component
persistent identity: component2354195
display id: component2354195
version: 1
top level: BBa_K1073033
definition: BBa_K1073019
access: public

BBa_K1073019
type: SequenceAnnotation
persistent identity: annotation2354195
display id: annotation2354195
version: 1
top level: BBa_K1073033
location: https://synbiohub.org/public/igem/BBa_K1073033/annotation2354195/range2354195/1
component: BBa_K1073019

https://synbiohub.org/public/igem/BBa_K1073033/annotation2354195/range2354195/1
type: Range
persistent identity: range2354195
display id: range2354195
version: 1
top level: BBa_K1073033
direction: direction/reverse
start: 1
end: 823
orientation: inline

BBa_K1073020
type: SequenceAnnotation
persistent identity: annotation2354203
display id: annotation2354203
version: 1
top level: BBa_K1073033
location: https://synbiohub.org/public/igem/BBa_K1073033/annotation2354203/range2354203/1
component: BBa_K1073020

https://synbiohub.org/public/igem/BBa_K1073033/annotation2354203/range2354203/1
type: Range
persistent identity: range2354203
display id: range2354203
version: 1
top level: BBa_K1073033
direction: direction/reverse
start: 832
end: 1592
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2354210
display id: annotation2354210
version: 1
top level: BBa_K1073033
location: https://synbiohub.org/public/igem/BBa_K1073033/annotation2354210/range2354210/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1073033/annotation2354210/range2354210/1
type: Range
persistent identity: range2354210
display id: range2354210
version: 1
top level: BBa_K1073033
direction: direction/reverse
start: 1601
end: 1729
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1073019
type: ComponentDefinition
persistent identity: BBa K1073019
display id: BBa_K1073019
version: 1
was generated by: iGEM to SBOL conversion
description: Autoinducer synthetase RhlI ( producing N-butyryl-HSL) with RBS and double terminator
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: This part is a combination of BBa_B0032, BBa_C0070 and BBa_B0015, which were taken from the 2013 distribution kit.
owned by: james
owned by: myers
mutable description: To the synthetase for the autoinducer N-butyryl-HSL, which binds to RhlR, (Part:BBa_C0071) a RBS and a double terminator was added to facilitate the construction of an expression system.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0032
component: BBa_B0015
component: BBa_C0070
sequence annotation: BBa_B0032
sequence annotation: BBa_C0070
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1073019_sequence/1

BBa_B0032
type: Component
persistent identity: component2349336
display id: component2349336
version: 1
top level: BBa_K1073019
definition: BBa_B0032
access: public

BBa_B0015
type: Component
persistent identity: component2349348
display id: component2349348
version: 1
top level: BBa_K1073019
definition: BBa_B0015
access: public

BBa_C0070
type: Component
persistent identity: component2349341
display id: component2349341
version: 1
top level: BBa_K1073019
definition: rhII
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2349336
display id: annotation2349336
version: 1
top level: BBa_K1073019
location: https://synbiohub.org/public/igem/BBa_K1073019/annotation2349336/range2349336/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1073019/annotation2349336/range2349336/1
type: Range
persistent identity: range2349336
display id: range2349336
version: 1
top level: BBa_K1073019
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2349341
display id: annotation2349341
version: 1
top level: BBa_K1073019
location: https://synbiohub.org/public/igem/BBa_K1073019/annotation2349341/range2349341/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_K1073019/annotation2349341/range2349341/1
type: Range
persistent identity: range2349341
display id: range2349341
version: 1
top level: BBa_K1073019
direction: direction/reverse
start: 20
end: 686
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2349348
display id: annotation2349348
version: 1
top level: BBa_K1073019
location: https://synbiohub.org/public/igem/BBa_K1073019/annotation2349348/range2349348/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1073019/annotation2349348/range2349348/1
type: Range
persistent identity: range2349348
display id: range2349348
version: 1
top level: BBa_K1073019
direction: direction/reverse
start: 695
end: 823
orientation: inline

aeblue
type: ComponentDefinition
persistent identity: BBa K1073020
display id: BBa_K1073020
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutively expressed chromoprotein aeBlue
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: This part is a combination of BBa_K1073006 and BBa_K864401, which were taken from the 2013 distribution kit and the iGEM Team Uppsala.
owned by: james
owned by: myers
mutable description: The chromoprotein aeblue is constitutively expressed and can be used for the marking of cells for easier differentiation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_K864401
component: BBa_K1073006
sequence annotation: BBa_K1073006
sequence annotation: BBa_K864401
sequence: https://synbiohub.org/public/igem/BBa_K1073020_sequence/1

BBa_K864401
type: Component
persistent identity: component2354072
display id: component2354072
version: 1
top level: aeblue
definition: aeBlue
access: public

BBa_K1073006
type: Component
persistent identity: component2354070
display id: component2354070
version: 1
top level: aeblue
definition: BBa_K1073006
access: public

BBa_K1073006
type: SequenceAnnotation
persistent identity: annotation2354070
display id: annotation2354070
version: 1
top level: aeblue
location: https://synbiohub.org/public/igem/BBa_K1073020/annotation2354070/range2354070/1
component: BBa_K1073006

https://synbiohub.org/public/igem/BBa_K1073020/annotation2354070/range2354070/1
type: Range
persistent identity: range2354070
display id: range2354070
version: 1
top level: aeblue
direction: direction/reverse
start: 1
end: 56
orientation: inline

BBa_K864401
type: SequenceAnnotation
persistent identity: annotation2354072
display id: annotation2354072
version: 1
top level: aeblue
location: https://synbiohub.org/public/igem/BBa_K1073020/annotation2354072/range2354072/1
component: BBa_K864401

https://synbiohub.org/public/igem/BBa_K1073020/annotation2354072/range2354072/1
type: Range
persistent identity: range2354072
display id: range2354072
version: 1
top level: aeblue
direction: direction/reverse
start: 63
end: 761
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

rhII
type: ComponentDefinition
persistent identity: BBa C0070
display id: BBa_C0070
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for N-butyryl-HSL (BHL) and HHL
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: P. aeruginosa PA3476
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Autoinducer synthesis protein that produces N-butyryl-HSL which binds to RhlR, obtained from Pseudomonas aeruginosa.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Editing Check (1/28/04) (0) BB sites cleaned (1) ATG (2) TAATAA (3) Blast checks out (i.e., it's RhlI) (4) Codon changes OK (AA and tRNA usage) (5) Chassis might have conflict (w/ native autoinducer system?) (6) ssrA tag added (7) barcode added (8) Codon optimized for expression in enteric bacteria 2 silent point mutations have been included into the sequence at base 138 from A to G and at base 576 from G to C to remove internal EcoRI and PstI sites. Mutations were chosen to yield the same amino acid and codons of similar frequency in E. coli.
star: true
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rhlL
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0070_sequence/1

rhlL
type: SequenceAnnotation
persistent identity: annotation301203
display id: annotation301203
version: 1
top level: rhII
location: https://synbiohub.org/public/igem/BBa_C0070/annotation301203/range301203/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0070/annotation301203/range301203/1
type: Range
persistent identity: range301203
display id: range301203
version: 1
top level: rhII
direction: direction/forward
start: 1
end: 603
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306820
display id: annotation306820
version: 1
top level: rhII
location: https://synbiohub.org/public/igem/BBa_C0070/annotation306820/range306820/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0070/annotation306820/range306820/1
type: Range
persistent identity: range306820
display id: range306820
version: 1
top level: rhII
direction: direction/forward
start: 604
end: 636
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213996
display id: annotation2213996
version: 1
top level: rhII
location: https://synbiohub.org/public/igem/BBa_C0070/annotation2213996/range2213996/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0070/annotation2213996/range2213996/1
type: Range
persistent identity: range2213996
display id: range2213996
version: 1
top level: rhII
direction: direction/forward
start: 643
end: 667
orientation: inline

aeBlue
type: ComponentDefinition
persistent identity: BBa K864401
display id: BBa_K864401
version: 1
was generated by: iGEM to SBOL conversion
description: aeBlue blue chromoprotein
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
mutable provenance: Source
owned by: james
owned by: myers
mutable description: aeBlue
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Synthesized and codon optimized for E. coli by Bioneer Corp.
star: false
creator: Erik Lundin
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aeBlue
sequence: https://synbiohub.org/public/igem/BBa_K864401_sequence/1

aeBlue
type: SequenceAnnotation
persistent identity: annotation2197206
display id: annotation2197206
version: 1
top level: aeBlue
location: https://synbiohub.org/public/igem/BBa_K864401/annotation2197206/range2197206/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K864401/annotation2197206/range2197206/1
type: Range
persistent identity: range2197206
display id: range2197206
version: 1
top level: aeBlue
direction: direction/forward
start: 1
end: 699
orientation: inline

BBa_K1073006
type: ComponentDefinition
persistent identity: BBa K1073006
display id: BBa_K1073006
version: 1
was generated by: iGEM to SBOL conversion
description: strong constitutive promoter with RBS
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: This part is a combination of BBa_J23106 and BBa_B0032, which were taken from 2013 distribution kit.
owned by: james
owned by: myers
mutable description: This part combines a constitutive promoter with an RBS and can be cloned in front of a gene of interest. It is a combination of BBa_J23106 and BBa_B0032.
discontinued: false
dominant: false
experience: experience/None
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0032
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K1073006_sequence/1

BBa_B0032
type: Component
persistent identity: component2352910
display id: component2352910
version: 1
top level: BBa_K1073006
definition: BBa_B0032
access: public

BBa_J23100
type: Component
persistent identity: component2352908
display id: component2352908
version: 1
top level: BBa_K1073006
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2352908
display id: annotation2352908
version: 1
top level: BBa_K1073006
location: https://synbiohub.org/public/igem/BBa_K1073006/annotation2352908/range2352908/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1073006/annotation2352908/range2352908/1
type: Range
persistent identity: range2352908
display id: range2352908
version: 1
top level: BBa_K1073006
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2352910
display id: annotation2352910
version: 1
top level: BBa_K1073006
location: https://synbiohub.org/public/igem/BBa_K1073006/annotation2352910/range2352910/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1073006/annotation2352910/range2352910/1
type: Range
persistent identity: range2352910
display id: range2352910
version: 1
top level: BBa_K1073006
direction: direction/forward
start: 44
end: 56
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_K1073033_sequence/1
type: Sequence
persistent identity: BBa K1073033 sequence
display id: BBa_K1073033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatggcttcactggttaaaaaagacatgtgcatcaaaatgacgatggaaggaacagtaaacggtcaccatttcaagtgtgtaggagaaggcgaaggcaaaccatttgaagggacccaggtggaaaagatacgcatcactgaaggtgggcccttaccatttgcgtatgatattttggccccttgttgcatgtatggcagtaaaaccttcattaagcatgtgtcgggtattccggattactttaaggagtcttttcctgagggctttacctgggaaagaacacaaatcttcgaggatggcggctatctcaccatacaccaggacacgagccttcagggtaataattttattttcaaagttaatgtcatcggtgccaacttccctgcaaacggtcccgtgatgcagaaaaaaacagctggatgggaaccgtgcgttgagatgctttatccgcgggacggcgtcctgtgtggtcagagcctgatggccctgaaatgcactgatggcaatcatctgacgtcccacctgcgcactacctatcgttctcgcaagccatccaatgcagttaacatgccggaatttcattttggggatcatcgcattgagattttgaaagctgaacaaggtaaattttatgaacaatacgagtcagcggtggcccgttactgtgaggcggcaccgagtaaattagggcatcactaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073019_sequence/1
type: Sequence
persistent identity: BBa K1073019 sequence
display id: BBa_K1073019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073020_sequence/1
type: Sequence
persistent identity: BBa K1073020 sequence
display id: BBa_K1073020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatggcttcactggttaaaaaagacatgtgcatcaaaatgacgatggaaggaacagtaaacggtcaccatttcaagtgtgtaggagaaggcgaaggcaaaccatttgaagggacccaggtggaaaagatacgcatcactgaaggtgggcccttaccatttgcgtatgatattttggccccttgttgcatgtatggcagtaaaaccttcattaagcatgtgtcgggtattccggattactttaaggagtcttttcctgagggctttacctgggaaagaacacaaatcttcgaggatggcggctatctcaccatacaccaggacacgagccttcagggtaataattttattttcaaagttaatgtcatcggtgccaacttccctgcaaacggtcccgtgatgcagaaaaaaacagctggatgggaaccgtgcgttgagatgctttatccgcgggacggcgtcctgtgtggtcagagcctgatggccctgaaatgcactgatggcaatcatctgacgtcccacctgcgcactacctatcgttctcgcaagccatccaatgcagttaacatgccggaatttcattttggggatcatcgcattgagattttgaaagctgaacaaggtaaattttatgaacaatacgagtcagcggtggcccgttactgtgaggcggcaccgagtaaattagggcatcactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0070_sequence/1
type: Sequence
persistent identity: BBa C0070 sequence
display id: BBa_C0070_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K864401_sequence/1
type: Sequence
persistent identity: BBa K864401 sequence
display id: BBa_K864401_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcactggttaaaaaagacatgtgcatcaaaatgacgatggaaggaacagtaaacggtcaccatttcaagtgtgtaggagaaggcgaaggcaaaccatttgaagggacccaggtggaaaagatacgcatcactgaaggtgggcccttaccatttgcgtatgatattttggccccttgttgcatgtatggcagtaaaaccttcattaagcatgtgtcgggtattccggattactttaaggagtcttttcctgagggctttacctgggaaagaacacaaatcttcgaggatggcggctatctcaccatacaccaggacacgagccttcagggtaataattttattttcaaagttaatgtcatcggtgccaacttccctgcaaacggtcccgtgatgcagaaaaaaacagctggatgggaaccgtgcgttgagatgctttatccgcgggacggcgtcctgtgtggtcagagcctgatggccctgaaatgcactgatggcaatcatctgacgtcccacctgcgcactacctatcgttctcgcaagccatccaatgcagttaacatgccggaatttcattttggggatcatcgcattgagattttgaaagctgaacaaggtaaattttatgaacaatacgagtcagcggtggcccgttactgtgaggcggcaccgagtaaattagggcatcactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073006_sequence/1
type: Sequence
persistent identity: BBa K1073006 sequence
display id: BBa_K1073006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z