Autoinducer synthase RhlI + LVA with combined aeBlue chromoprotein expression cassette
The biobricks for this part were derived from the 2013 distribution kit and the iGEM Team Uppsala.
This part is the basis for the expression of the Rhl auto inducer synthetase, which synthesizes RhlI [N-(butyryl)-homoserine lactone]. RhlI binds to the activator RhlR. Together they activate the Rhl promoter. A constitutive expression of aeblue is added to the construct in order to varify the expression of RhlI synthetase. The construct has no promoter in front of the RhlI synthetase gene. Therefore the user can choose the promoter himself (e.g. constitutive, induced, repressed, etc.)
Double terminator consisting of BBa_B0010 and BBa_B0012
Autoinducer synthetase RhlI ( producing N-butyryl-HSL) with RBS and double terminator
This part is a combination of BBa_B0032, BBa_C0070 and BBa_B0015, which were taken from the 2013 distribution kit.
To the synthetase for the autoinducer N-butyryl-HSL, which binds to RhlR, (Part:BBa_C0071) a RBS and a double terminator was added to facilitate the construction of an expression system.
Constitutively expressed chromoprotein aeBlue
This part is a combination of BBa_K1073006 and BBa_K864401, which were taken from the 2013 distribution kit and the iGEM Team Uppsala.
The chromoprotein aeblue is constitutively expressed and can be used for the marking of cells for easier differentiation.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
RBS.3 (medium) -- derivative of BBa_0030
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Transcriptional terminator consisting of a 64 bp stem-loop.
autoinducer synthetase for N-butyryl-HSL (BHL) and HHL
Autoinducer synthesis protein that produces N-butyryl-HSL which binds to RhlR, obtained from Pseudomonas aeruginosa.
Editing Check (1/28/04) (0) BB sites cleaned (1) ATG (2) TAATAA (3) Blast checks out (i.e., it's RhlI) (4) Codon changes OK (AA and tRNA usage) (5) Chassis might have conflict (w/ native autoinducer system?) (6) ssrA tag added (7) barcode added (8) Codon optimized for expression in enteric bacteria 2 silent point mutations have been included into the sequence at base 138 from A to G and at base 576 from G to C to remove internal EcoRI and PstI sites. Mutations were chosen to yield the same amino acid and codons of similar frequency in E. coli.
Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
Synthesized and codon optimized for E. coli by Bioneer Corp.
This part is a combination of BBa_J23106 and BBa_B0032, which were taken from 2013 distribution kit.
This part combines a constitutive promoter with an RBS and can be cloned in front of a gene of interest. It is a combination of BBa_J23106 and BBa_B0032.
Conversion of the iGEM parts registry to SBOL2.1