LOCUS BBa_K1073034 3879 bp DNA linear 22-Dec-2024 DEFINITION Inducible ampR expression combined with LasR, RhlI and aeBlue expression cassettes ACCESSION BBa_K1073034 VERSION BBa_K1073034.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 terminator 1..129 /label=BBa_B0015 misc_feature 223..228 /label=-35 Region misc_feature 212..230 /label=LasR Binding Site misc_feature 250..255 /label= -10 Region misc_feature 262..262 /label=Primary Transcriptional Start promoter 138..263 /label=BBa_K091117 RBS 272..284 /label=RBS-3\Weak RBS 278..281 /label=RBS misc_feature 272..284 /label=BBa_B0032 RBS 272..284 /label=BBa_B0032 promoter 138..284 /label=BBa_K1073003 CDS 291..1154 /label=BBa_K1073000 misc_feature 138..1154 /label=BBa_K1073013 stem_loop 1174..1217 /label=stem_loop misc_feature 1163..1242 /label=BBa_B0010 terminator 1163..1242 /label=BBa_B0010 stem_loop 1258..1277 /label=T7 TE misc_feature 1284..1284 /label=stop polyA_site 1278..1291 /label=polya misc_feature 1251..1291 /label=BBa_B0012 terminator 1251..1291 /label=BBa_B0012 terminator 1163..1291 /label=BBa_B0015 promoter 1300..1334 /label=BBa_J23100 RBS 1343..1355 /label=RBS-3\Weak RBS 1349..1352 /label=RBS misc_feature 1343..1355 /label=BBa_B0032 RBS 1343..1355 /label=BBa_B0032 promoter 1300..1355 /label=BBa_K1073006 CDS 1362..2078 /label=lasR misc_feature 2079..2111 /label=LVA misc_feature 1841..1841 /label=G misc_feature 2118..2142 /label=Help:Barcodes CDS 1362..2142 /label=BBa_C0079 misc_feature 1300..2142 /label=BBa_K1073016 misc_feature 138..2142 /label=BBa_K1073030 RBS 2151..2163 /label=RBS-3\Weak RBS 2157..2160 /label=RBS misc_feature 2151..2163 /label=BBa_B0032 RBS 2151..2163 /label=BBa_B0032 CDS 2170..2772 /label=rhlL misc_feature 2773..2805 /label=LVA misc_feature 2812..2836 /label=Help:Barcodes CDS 2170..2836 /label=BBa_C0070 stem_loop 2856..2899 /label=stem_loop misc_feature 2845..2924 /label=BBa_B0010 terminator 2845..2924 /label=BBa_B0010 stem_loop 2940..2959 /label=T7 TE misc_feature 2966..2966 /label=stop polyA_site 2960..2973 /label=polya misc_feature 2933..2973 /label=BBa_B0012 terminator 2933..2973 /label=BBa_B0012 terminator 2845..2973 /label=BBa_B0015 misc_feature 2151..2973 /label=BBa_K1073019 promoter 2982..3016 /label=BBa_J23100 RBS 3025..3037 /label=RBS-3\Weak RBS 3031..3034 /label=RBS misc_feature 3025..3037 /label=BBa_B0032 RBS 3025..3037 /label=BBa_B0032 promoter 2982..3037 /label=BBa_K1073006 CDS 3044..3742 /label=aeBlue CDS 3044..3742 /label=BBa_K864401 misc_feature 2982..3742 /label=BBa_K1073020 stem_loop 3762..3805 /label=stem_loop misc_feature 3751..3830 /label=BBa_B0010 terminator 3751..3830 /label=BBa_B0010 stem_loop 3846..3865 /label=T7 TE misc_feature 3872..3872 /label=stop polyA_site 3866..3879 /label=polya misc_feature 3839..3879 /label=BBa_B0012 terminator 3839..3879 /label=BBa_B0012 terminator 3751..3879 /label=BBa_B0015 misc_feature 2151..3879 /label=BBa_K1073033 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagtgt tctcgtgtga agccattgct ctgatctttt ggacgtttct 181 tcgagcctag caagggtccg ggttcaccga aatctatctc atttgctagt tataaaatta 241 tgaaatttgt ataaattctt cagtactaga gtcacacagg aaagtactag atgagtattc 301 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 361 acccagaaac gctggtgaaa gtaaaagatg ccgaagatca gttgggtgca cgtgtgggtt 421 acatcgaact ggacctcaac agcggtaaga ttcttgagag ttttcgcccc gaagaacgtt 481 tcccaatgat gagcactttt aaagttctgc tctgtggcgc ggtattatcc cgtattgacg 541 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 601 caccagtcac agaaaagcat cttacggacg gcatgacagt acgcgaatta tgcagcgctg 661 ccataaccat gagtgataac acggcggcca acttacttct gacaacgatc ggaggaccga 721 aggagcttac cgcttttttg cacaacatgg gtgatcatgt aactcgcctt gatcgttggg 781 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcta 841 tggcaacaac gttgcgcaaa ctcttaactg gcgaacttct tactctcgct tcccggcaac 901 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 961 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtcc cgcggtatta 1021 ttgcagccct ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 1081 gccaggcaac tatggacgaa cgtaatcgcc agatcgctga gataggtgcc tccctgatta 1141 agcattggta ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 1201 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 1261 tcaccttcgg gtgggccttt ctgcgtttat atactagagt tgacggctag ctcagtccta 1321 ggtacagtgc tagctactag agtcacacag gaaagtacta gatggccttg gttgacggtt 1381 ttcttgagct ggaacgctca agtggaaaat tggagtggag cgccatcctc cagaagatgg 1441 cgagcgacct tggattctcg aagatcctgt tcggcctgtt gcctaaggac agccaggact 1501 acgagaacgc cttcatcgtc ggcaactacc cggccgcctg gcgcgagcat tacgaccggg 1561 ctggctacgc gcgggtcgac ccgacggtca gtcactgtac ccagagcgta ctgccgattt 1621 tctgggaacc gtccatctac cagacgcgaa agcagcacga gttcttcgag gaagcctcgg 1681 ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg tgctcgcggc gaactcggcg 1741 cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc caaccgtttc atagagtcgg 1801 tcctgccgac cctgtggatg ctcaaggact acgcactgca aagcggtgcc ggactggcct 1861 tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg ggagaaggaa gtgttgcagt 1921 ggtgcgccat cggcaagacc agttgggaga tatcggttat ctgcaactgc tcggaagcca 1981 atgtgaactt ccatatggga aatattcggc ggaagttcgg tgtgacctcc cgccgcgtag 2041 cggccattat ggccgttaat ttgggtctta ttactctcgc tgcaaacgac gaaaactacg 2101 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag tcacacagga 2161 aagtactaga tgatcgaact gctgtccgaa tccctggaag gtctgtccgc tgctatgatc 2221 gctgaactgg gtcgttaccg tcaccaggtt ttcatcgaaa aactgggttg ggacgttgtt 2281 tccacctccc gtgttcgtga ccaggagttc gaccagttcg accacccgca gacccgttac 2341 atcgttgcta tgtcccgtca gggtatctgc ggttgcgctc gtctgctgcc gaccaccgac 2401 gcttacctgc tgaaagacgt tttcgcttac ctgtgctccg aaaccccgcc gtccgacccg 2461 tccgtttggg aactgtcccg ttacgctgct tccgctgctg acgacccgca gctggctatg 2521 aaaatcttct ggtcctccct ccagtgcgct tggtacctgg gtgcttcctc cgttgttgct 2581 gttaccacca ccgctatgga acgttacttc gttcgtaacg gtgttatcct ccagcgtctg 2641 ggtccgccgc agaaagttaa aggtgaaacc ctggttgcta tctccttccc ggcttaccag 2701 gaacgtggtc tggaaatgct gctgcgttac cacccggaat ggctccaggg tgttccgctg 2761 tccatggctg ttgctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag 2821 tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 2881 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 2941 gctcaccttc gggtgggcct ttctgcgttt atatactaga gttgacggct agctcagtcc 3001 taggtacagt gctagctact agagtcacac aggaaagtac tagatggctt cactggttaa 3061 aaaagacatg tgcatcaaaa tgacgatgga aggaacagta aacggtcacc atttcaagtg 3121 tgtaggagaa ggcgaaggca aaccatttga agggacccag gtggaaaaga tacgcatcac 3181 tgaaggtggg cccttaccat ttgcgtatga tattttggcc ccttgttgca tgtatggcag 3241 taaaaccttc attaagcatg tgtcgggtat tccggattac tttaaggagt cttttcctga 3301 gggctttacc tgggaaagaa cacaaatctt cgaggatggc ggctatctca ccatacacca 3361 ggacacgagc cttcagggta ataattttat tttcaaagtt aatgtcatcg gtgccaactt 3421 ccctgcaaac ggtcccgtga tgcagaaaaa aacagctgga tgggaaccgt gcgttgagat 3481 gctttatccg cgggacggcg tcctgtgtgg tcagagcctg atggccctga aatgcactga 3541 tggcaatcat ctgacgtccc acctgcgcac tacctatcgt tctcgcaagc catccaatgc 3601 agttaacatg ccggaatttc attttgggga tcatcgcatt gagattttga aagctgaaca 3661 aggtaaattt tatgaacaat acgagtcagc ggtggcccgt tactgtgagg cggcaccgag 3721 taaattaggg catcactaat aatactagag ccaggcatca aataaaacga aaggctcagt 3781 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 3841 acactggctc accttcgggt gggcctttct gcgtttata //