##gff-version 3
##sequence-region BBa_K1073034 1 3879
BBa_K1073034	.	engineered_region	138	2142	.	+	0	ID=BBa_K1073030;Name=BBa_K1073030
BBa_K1073034	.	terminator	1163	1291	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1073030
BBa_K1073034	.	terminator	1163	1242	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1073034	.	engineered_region	1163	1242	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1073034	.	stem_loop	1174	1217	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1073034	.	terminator	1251	1291	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1073034	.	stop_codon	1284	1284	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1073034	.	polyA_site	1278	1291	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1073034	.	stem_loop	1258	1277	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1073034	.	engineered_region	1251	1291	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1073034	.	engineered_region	1300	2142	.	+	0	ID=BBa_K1073016;Name=BBa_K1073016;Parent=BBa_K1073030
BBa_K1073034	.	CDS	1362	2142	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_K1073016
BBa_K1073034	.	tag	2079	2111	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K1073034	.	CDS	1362	2078	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K1073034	.	sequence_alteration	1841	1841	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K1073034	.	engineered_tag	2118	2142	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K1073034	.	promoter	1300	1355	.	+	0	ID=BBa_K1073006;Name=BBa_K1073006;Parent=BBa_K1073016
BBa_K1073034	.	ribosome_entry_site	1343	1355	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1073006
BBa_K1073034	.	ribosome_entry_site	1343	1355	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1073034	.	engineered_region	1343	1355	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1073034	.	ribosome_entry_site	1349	1352	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1073034	.	promoter	1300	1334	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1073006
BBa_K1073034	.	engineered_region	138	1154	.	+	0	ID=BBa_K1073013;Name=BBa_K1073013;Parent=BBa_K1073030
BBa_K1073034	.	promoter	138	284	.	+	0	ID=BBa_K1073003;Name=BBa_K1073003;Parent=BBa_K1073013
BBa_K1073034	.	ribosome_entry_site	272	284	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1073003
BBa_K1073034	.	ribosome_entry_site	272	284	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1073034	.	engineered_region	272	284	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1073034	.	ribosome_entry_site	278	281	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1073034	.	promoter	138	263	.	+	0	ID=BBa_K091117;Name=BBa_K091117;Parent=BBa_K1073003
BBa_K1073034	.	start_codon	262	262	.	+	0	ID=annotation1964075;Name=Primary Transcriptional Start;Parent=BBa_K091117
BBa_K1073034	.	sequence_feature	223	228	.	+	0	ID=annotation1964072;Name=-35 Region;Parent=BBa_K091117
BBa_K1073034	.	non_covalent_binding_site	212	230	.	+	0	ID=annotation1964073;Name=LasR Binding Site;Parent=BBa_K091117
BBa_K1073034	.	sequence_feature	250	255	.	+	0	ID=annotation1964074;Name= -10 Region;Parent=BBa_K091117
BBa_K1073034	.	CDS	291	1154	.	+	0	ID=BBa_K1073000;Name=BBa_K1073000;Parent=BBa_K1073013
BBa_K1073034	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1073034	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1073034	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1073034	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1073034	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1073034	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1073034	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1073034	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1073034	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1073034	.	engineered_region	2151	3879	.	+	0	ID=BBa_K1073033;Name=BBa_K1073033
BBa_K1073034	.	terminator	3751	3879	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1073033
BBa_K1073034	.	terminator	3751	3830	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1073034	.	engineered_region	3751	3830	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1073034	.	stem_loop	3762	3805	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1073034	.	terminator	3839	3879	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1073034	.	stop_codon	3872	3872	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1073034	.	polyA_site	3866	3879	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1073034	.	stem_loop	3846	3865	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1073034	.	engineered_region	3839	3879	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1073034	.	engineered_region	2151	2973	.	+	0	ID=BBa_K1073019;Name=BBa_K1073019;Parent=BBa_K1073033
BBa_K1073034	.	terminator	2845	2973	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1073019
BBa_K1073034	.	terminator	2845	2924	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1073034	.	engineered_region	2845	2924	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1073034	.	stem_loop	2856	2899	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1073034	.	terminator	2933	2973	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1073034	.	stop_codon	2966	2966	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1073034	.	polyA_site	2960	2973	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1073034	.	stem_loop	2940	2959	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1073034	.	engineered_region	2933	2973	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1073034	.	ribosome_entry_site	2151	2163	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1073019
BBa_K1073034	.	ribosome_entry_site	2151	2163	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1073034	.	engineered_region	2151	2163	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1073034	.	ribosome_entry_site	2157	2160	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1073034	.	CDS	2170	2836	.	+	0	ID=BBa_C0070;Name=BBa_C0070;Parent=BBa_K1073019
BBa_K1073034	.	tag	2773	2805	.	+	0	ID=annotation306820;Name=LVA;Parent=BBa_C0070
BBa_K1073034	.	CDS	2170	2772	.	+	0	ID=annotation301203;Name=rhlL;Parent=BBa_C0070
BBa_K1073034	.	engineered_tag	2812	2836	.	+	0	ID=annotation2213996;Name=Help:Barcodes;Parent=BBa_C0070
BBa_K1073034	.	engineered_region	2982	3742	.	+	0	ID=BBa_K1073020;Name=BBa_K1073020;Parent=BBa_K1073033
BBa_K1073034	.	promoter	2982	3037	.	+	0	ID=BBa_K1073006;Name=BBa_K1073006;Parent=BBa_K1073020
BBa_K1073034	.	ribosome_entry_site	3025	3037	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1073006
BBa_K1073034	.	ribosome_entry_site	3025	3037	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1073034	.	engineered_region	3025	3037	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1073034	.	ribosome_entry_site	3031	3034	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1073034	.	promoter	2982	3016	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1073006
BBa_K1073034	.	CDS	3044	3742	.	+	0	ID=BBa_K864401;Name=BBa_K864401;Parent=BBa_K1073020
BBa_K1073034	.	CDS	3044	3742	.	+	0	ID=annotation2197206;Name=aeBlue;Parent=BBa_K864401
>BBa_K1073034
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtgttctcgtgtgaagccattg
ctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagttataaaa
ttatgaaatttgtataaattcttcagtactagagtcacacaggaaagtactagatgagtattcaacatttccgtgtcgc
ccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgccgaa
gatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttgagagttttcgccccgaag
aacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtattgacgccgggcaagagca
actcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggacggc
atgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaacttacttctgacaacgatcg
gaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgatcgttgggaaccggagct
gaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgttgcgcaaactcttaact
ggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgc
gctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcccgcggtattattgcagc
cctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaactatggacgaacgtaat
cgccagatcgctgagataggtgcctccctgattaagcattggtaataatactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagt
cacacaggaaagtactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcg
ccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggacta
cgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgac
ccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacg
agttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcgg
cgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtgg
atgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgacca
gccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcgga
agccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggcc
gttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagt
gtagatcactactagagtcacacaggaaagtactagatgatcgaactgctgtccgaatccctggaaggtctgtccgctg
ctatgatcgctgaactgggtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccg
tgttcgtgaccaggagttcgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgc
ggttgcgctcgtctgctgccgaccaccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgc
cgtccgacccgtccgtttgggaactgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatctt
ctggtcctccctccagtgcgcttggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttac
ttcgttcgtaacggtgttatcctccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctcct
tcccggcttaccaggaacgtggtctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccat
ggctgttgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtccta
ggtacagtgctagctactagagtcacacaggaaagtactagatggcttcactggttaaaaaagacatgtgcatcaaaat
gacgatggaaggaacagtaaacggtcaccatttcaagtgtgtaggagaaggcgaaggcaaaccatttgaagggacccag
gtggaaaagatacgcatcactgaaggtgggcccttaccatttgcgtatgatattttggccccttgttgcatgtatggca
gtaaaaccttcattaagcatgtgtcgggtattccggattactttaaggagtcttttcctgagggctttacctgggaaag
aacacaaatcttcgaggatggcggctatctcaccatacaccaggacacgagccttcagggtaataattttattttcaaa
gttaatgtcatcggtgccaacttccctgcaaacggtcccgtgatgcagaaaaaaacagctggatgggaaccgtgcgttg
agatgctttatccgcgggacggcgtcctgtgtggtcagagcctgatggccctgaaatgcactgatggcaatcatctgac
gtcccacctgcgcactacctatcgttctcgcaagccatccaatgcagttaacatgccggaatttcattttggggatcat
cgcattgagattttgaaagctgaacaaggtaaattttatgaacaatacgagtcagcggtggcccgttactgtgaggcgg
caccgagtaaattagggcatcactaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttata