LOCUS BBa_K1073035 3657 bp DNA linear 22-Dec-2024 DEFINITION Inducible ampR expression combined with RhlR, LasI and eforRed expression cassettes ACCESSION BBa_K1073035 VERSION BBa_K1073035.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=RhlR promoter 19..24 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start promoter 1..53 /label=BBa_R0071 RBS 62..74 /label=RBS-3\Weak RBS 68..71 /label=RBS misc_feature 62..74 /label=BBa_B0032 RBS 62..74 /label=BBa_B0032 promoter 1..74 /label=BBa_K1073001 CDS 81..944 /label=BBa_K1073000 misc_feature 1..944 /label=BBa_K1073011 stem_loop 964..1007 /label=stem_loop misc_feature 953..1032 /label=BBa_B0010 terminator 953..1032 /label=BBa_B0010 stem_loop 1048..1067 /label=T7 TE misc_feature 1074..1074 /label=stop polyA_site 1068..1081 /label=polya misc_feature 1041..1081 /label=BBa_B0012 terminator 1041..1081 /label=BBa_B0012 terminator 953..1081 /label=BBa_B0015 promoter 1090..1124 /label=BBa_J23100 RBS 1133..1145 /label=RBS-3\Weak RBS 1139..1142 /label=RBS misc_feature 1133..1145 /label=BBa_B0032 RBS 1133..1145 /label=BBa_B0032 promoter 1090..1145 /label=BBa_K1073006 CDS 1152..1874 /label=rhlR misc_feature 1875..1907 /label=LVA misc_feature 1914..1938 /label=Help:Barcodes CDS 1152..1938 /label=BBa_C0071 misc_feature 1090..1938 /label=BBa_K1073014 misc_feature 1..1938 /label=BBa_K1073028 RBS 1947..1959 /label=RBS-3\Weak RBS 1953..1956 /label=RBS misc_feature 1947..1959 /label=BBa_B0032 RBS 1947..1959 /label=BBa_B0032 CDS 1966..2568 /label=LasI misc_feature 2569..2601 /label=LVA misc_feature 2608..2632 /label=Help:Barcodes CDS 1966..2632 /label=BBa_C0078 stem_loop 2652..2695 /label=stem_loop misc_feature 2641..2720 /label=BBa_B0010 terminator 2641..2720 /label=BBa_B0010 stem_loop 2736..2755 /label=T7 TE misc_feature 2762..2762 /label=stop polyA_site 2756..2769 /label=polya misc_feature 2729..2769 /label=BBa_B0012 terminator 2729..2769 /label=BBa_B0012 terminator 2641..2769 /label=BBa_B0015 misc_feature 1947..2769 /label=BBa_K1073018 promoter 2778..2812 /label=BBa_J23100 RBS 2821..2833 /label=RBS-3\Weak RBS 2827..2830 /label=RBS misc_feature 2821..2833 /label=BBa_B0032 RBS 2821..2833 /label=BBa_B0032 promoter 2778..2833 /label=BBa_K1073006 CDS 2840..3520 /label=eforRed CDS 2840..3520 /label=BBa_K592012 misc_feature 2778..3520 /label=BBa_K1073022 stem_loop 3540..3583 /label=stem_loop misc_feature 3529..3608 /label=BBa_B0010 terminator 3529..3608 /label=BBa_B0010 stem_loop 3624..3643 /label=T7 TE misc_feature 3650..3650 /label=stop polyA_site 3644..3657 /label=polya misc_feature 3617..3657 /label=BBa_B0012 terminator 3617..3657 /label=BBa_B0012 terminator 3529..3657 /label=BBa_B0015 misc_feature 1947..3657 /label=BBa_K1073032 ORIGIN 1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga 61 gtcacacagg aaagtactag atgagtattc aacatttccg tgtcgccctt attccctttt 121 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 181 ccgaagatca gttgggtgca cgtgtgggtt acatcgaact ggacctcaac agcggtaaga 241 ttcttgagag ttttcgcccc gaagaacgtt tcccaatgat gagcactttt aaagttctgc 301 tctgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 361 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggacg 421 gcatgacagt acgcgaatta tgcagcgctg ccataaccat gagtgataac acggcggcca 481 acttacttct gacaacgatc ggaggaccga aggagcttac cgcttttttg cacaacatgg 541 gtgatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 601 acgagcgtga caccacgatg cctgtagcta tggcaacaac gttgcgcaaa ctcttaactg 661 gcgaacttct tactctcgct tcccggcaac aattaataga ctggatggag gcggataaag 721 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 781 gagccggtga gcgtgggtcc cgcggtatta ttgcagccct ggggccagat ggtaagccct 841 cccgtatcgt agttatctac acgacgggga gccaggcaac tatggacgaa cgtaatcgcc 901 agatcgctga gataggtgcc tccctgatta agcattggta ataatactag agccaggcat 961 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 1021 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 1081 atactagagt tgacggctag ctcagtccta ggtacagtgc tagctactag agtcacacag 1141 gaaagtacta gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg 1201 agatgcagcc gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc 1261 gcctgggctt cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga 1321 agaccgaggt ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact 1381 acggggccgt ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga 1441 gcgacagcct gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct 1501 gtgtcggcgc gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg 1561 cgcgcgacca gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt 1621 gcatgatcga gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca 1681 acccggtctg cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga 1741 gttccgggga aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca 1801 agaacatcca gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg 1861 cgctgggtct catcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat 1921 agtgctagtg tagatcacta ctagagtcac acaggaaagt actagatgat cgttcagatc 1981 ggtcgtcgtg aagagttcga caaaaaactg ctgggtgaaa tgcacaaact gcgtgctcag 2041 gttttcaaag aacgtaaagg ttgggacgtt tccgttatcg acgaaatgga aatcgacggt 2101 tacgacgctc tgtccccgta ctacatgctg atccaggaag acaccccgga agctcaggtt 2161 ttcggttgct ggcgtatctt cgacaccacc ggtccgtaca tgctgaaaaa caccttcccg 2221 gaactgctgc acggtaaaga agctccgtgc tccccgcaca tctgggaact gtcccgtttc 2281 gctatcaact ccggtcagaa aggttccctg ggtttctccg actgcaccct ggaagctatg 2341 cgtgctctgg ctcgttactc cttgcagaac gacatccaga ccctggttac cgttaccacc 2401 gttggtgttg aaaaaatgat gatccgtgct ggtctggacg tttcccgttt cggtccgcac 2461 ctgaaaatcg gtatcgaacg tgctgttgct ctgcgtatcg aactgaacgc taaaacccag 2521 atcgctctgt acggtggtgt tctggttgaa cagcgtctgg ctgtttccgc tgctaacgac 2581 gaaaactacg ctctggttgc ttaataactc tgatagtgct agtgtagatc tctactagag 2641 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 2701 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 2761 gcgtttatat actagagttg acggctagct cagtcctagg tacagtgcta gctactagag 2821 tcacacagga aagtactaga tgtcagtgat taagcaggta atgaagacca agttgcacct 2881 tgagggcact gtcaatggcc atgattttac gatcgagggt aaaggtgaag gcaagccgta 2941 cgaagggtta cagcacatga aaatgacagt caccaaaggc gcgcctctgc cgttttccgt 3001 tcatattctt acacctagcc acatgtatgg aagcaaaccg tttaataagt atccagcgga 3061 tatcccagac taccacaaac agtcttttcc cgaaggtatg tcttgggagc ggtcgatgat 3121 ttttgaagat ggtggcgtat gcaccgccag taatcactcc agcataaact tgcaagagaa 3181 ctgtttcatc tatgatgtta aatttcatgg tgtgaacctg cctccggatg ggcccgtaat 3241 gcaaaaaacc attgctggat gggagccgag cgtggaaaca ctgtacgtgc gtgacgggat 3301 gttaaaaagt gacactgcaa tggtttttaa actgaaagga ggcggtcatc atcgtgttga 3361 tttcaaaacg acgtataaag ccaaaaaacc tgtcaagctg ccagaatttc atttcgttga 3421 acatcgcctg gaactgacca aacacgataa agatttcaca acttgggacc agcaggaggc 3481 agccgaaggc catttctcac cgctgccgaa ggctctccca tactagagcc aggcatcaaa 3541 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 3601 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //