##gff-version 3
##sequence-region BBa_K1073035 1 3657
BBa_K1073035	.	engineered_region	1	1938	.	+	0	ID=BBa_K1073028;Name=BBa_K1073028
BBa_K1073035	.	terminator	953	1081	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1073028
BBa_K1073035	.	terminator	1041	1081	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1073035	.	polyA_site	1068	1081	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1073035	.	stem_loop	1048	1067	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1073035	.	stop_codon	1074	1074	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1073035	.	engineered_region	1041	1081	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1073035	.	terminator	953	1032	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1073035	.	stem_loop	964	1007	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1073035	.	engineered_region	953	1032	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1073035	.	engineered_region	1	944	.	+	0	ID=BBa_K1073011;Name=BBa_K1073011;Parent=BBa_K1073028
BBa_K1073035	.	promoter	1	74	.	+	0	ID=BBa_K1073001;Name=BBa_K1073001;Parent=BBa_K1073011
BBa_K1073035	.	ribosome_entry_site	62	74	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1073001
BBa_K1073035	.	ribosome_entry_site	62	74	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1073035	.	ribosome_entry_site	68	71	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1073035	.	engineered_region	62	74	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1073035	.	promoter	1	53	.	+	0	ID=BBa_R0071;Name=BBa_R0071;Parent=BBa_K1073001
BBa_K1073035	.	promoter	19	24	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K1073035	.	promoter	42	47	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K1073035	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K1073035	.	start_codon	53	53	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K1073035	.	CDS	81	944	.	+	0	ID=BBa_K1073000;Name=BBa_K1073000;Parent=BBa_K1073011
BBa_K1073035	.	engineered_region	1090	1938	.	+	0	ID=BBa_K1073014;Name=BBa_K1073014;Parent=BBa_K1073028
BBa_K1073035	.	CDS	1152	1938	.	+	0	ID=BBa_C0071;Name=BBa_C0071;Parent=BBa_K1073014
BBa_K1073035	.	engineered_tag	1914	1938	.	+	0	ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071
BBa_K1073035	.	CDS	1152	1874	.	+	0	ID=annotation301192;Name=rhlR;Parent=BBa_C0071
BBa_K1073035	.	tag	1875	1907	.	+	0	ID=annotation306523;Name=LVA;Parent=BBa_C0071
BBa_K1073035	.	promoter	1090	1145	.	+	0	ID=BBa_K1073006;Name=BBa_K1073006;Parent=BBa_K1073014
BBa_K1073035	.	ribosome_entry_site	1133	1145	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1073006
BBa_K1073035	.	ribosome_entry_site	1133	1145	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1073035	.	ribosome_entry_site	1139	1142	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1073035	.	engineered_region	1133	1145	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1073035	.	promoter	1090	1124	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1073006
BBa_K1073035	.	engineered_region	1947	3657	.	+	0	ID=BBa_K1073032;Name=BBa_K1073032
BBa_K1073035	.	terminator	3529	3657	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1073032
BBa_K1073035	.	terminator	3617	3657	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1073035	.	polyA_site	3644	3657	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1073035	.	stem_loop	3624	3643	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1073035	.	stop_codon	3650	3650	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1073035	.	engineered_region	3617	3657	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1073035	.	terminator	3529	3608	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1073035	.	stem_loop	3540	3583	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1073035	.	engineered_region	3529	3608	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1073035	.	engineered_region	2778	3520	.	+	0	ID=BBa_K1073022;Name=BBa_K1073022;Parent=BBa_K1073032
BBa_K1073035	.	CDS	2840	3520	.	+	0	ID=BBa_K592012;Name=BBa_K592012;Parent=BBa_K1073022
BBa_K1073035	.	CDS	2840	3520	.	+	0	ID=annotation2131799;Name=eforRed;Parent=BBa_K592012
BBa_K1073035	.	promoter	2778	2833	.	+	0	ID=BBa_K1073006;Name=BBa_K1073006;Parent=BBa_K1073022
BBa_K1073035	.	ribosome_entry_site	2821	2833	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1073006
BBa_K1073035	.	ribosome_entry_site	2821	2833	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1073035	.	ribosome_entry_site	2827	2830	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1073035	.	engineered_region	2821	2833	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1073035	.	promoter	2778	2812	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1073006
BBa_K1073035	.	engineered_region	1947	2769	.	+	0	ID=BBa_K1073018;Name=BBa_K1073018;Parent=BBa_K1073032
BBa_K1073035	.	CDS	1966	2632	.	+	0	ID=BBa_C0078;Name=BBa_C0078;Parent=BBa_K1073018
BBa_K1073035	.	tag	2569	2601	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_K1073035	.	CDS	1966	2568	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_K1073035	.	engineered_tag	2608	2632	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_K1073035	.	terminator	2641	2769	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1073018
BBa_K1073035	.	terminator	2729	2769	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1073035	.	polyA_site	2756	2769	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1073035	.	stem_loop	2736	2755	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1073035	.	stop_codon	2762	2762	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1073035	.	engineered_region	2729	2769	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1073035	.	terminator	2641	2720	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1073035	.	stem_loop	2652	2695	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1073035	.	engineered_region	2641	2720	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1073035	.	ribosome_entry_site	1947	1959	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1073018
BBa_K1073035	.	ribosome_entry_site	1947	1959	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1073035	.	ribosome_entry_site	1953	1956	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1073035	.	engineered_region	1947	1959	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
>BBa_K1073035
tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagtcacacaggaaagtacta
gatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaa
acgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggta
agattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtatt
atcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcacca
gtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacgg
cggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaac
tcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatg
gcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggagg
cggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtga
gcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacgggg
agccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataatact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagt
cctaggtacagtgctagctactagagtcacacaggaaagtactagatgaggaatgacggaggctttttgctgtggtggg
acggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcct
gggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctat
cccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcct
cggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctg
tgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatc
tccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctgg
agcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaa
gagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaag
ttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacg
ctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacaggaaagtactagatgatcgttc
agatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacg
taaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatc
caggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaaca
ccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactc
cggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaac
gacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtt
tcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctct
gtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataa
ctctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatgtc
agtgattaagcaggtaatgaagaccaagttgcaccttgagggcactgtcaatggccatgattttacgatcgagggtaaa
ggtgaaggcaagccgtacgaagggttacagcacatgaaaatgacagtcaccaaaggcgcgcctctgccgttttccgttc
atattcttacacctagccacatgtatggaagcaaaccgtttaataagtatccagcggatatcccagactaccacaaaca
gtcttttcccgaaggtatgtcttgggagcggtcgatgatttttgaagatggtggcgtatgcaccgccagtaatcactcc
agcataaacttgcaagagaactgtttcatctatgatgttaaatttcatggtgtgaacctgcctccggatgggcccgtaa
tgcaaaaaaccattgctggatgggagccgagcgtggaaacactgtacgtgcgtgacgggatgttaaaaagtgacactgc
aatggtttttaaactgaaaggaggcggtcatcatcgtgttgatttcaaaacgacgtataaagccaaaaaacctgtcaag
ctgccagaatttcatttcgttgaacatcgcctggaactgaccaaacacgataaagatttcacaacttgggaccagcagg
aggcagccgaaggccatttctcaccgctgccgaaggctctcccatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata