BBa_K1073035
type: ComponentDefinition
persistent identity: BBa K1073035
display id: BBa_K1073035
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible ampR expression combined with RhlR, LasI and eforRed expression cassettes
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: The parts for this construct were derived from the 2013 distribution kit and the iGEM Team Uppsala.
owned by: james
owned by: myers
mutable description: This construct is the basis for a Quorum sensing system for two bacteria. The selection marker (amp-resistance) is induced by a RhlI(inducer)/RhlR(activator) complex. The RhlR is encoded on the construct itself, whereas the RhlI is taken up from the media. Furthermore the LasI synthetase is expressed to synthesize the autoinducer LasI for the corresponding system in another cell (BBa_K1073034). By the activation of the amp-resistance the gene of interest (in this case the chromoprotein eforRed) is expressed. Together with BBa_K1073034 the construct forms a dependency between two cells (one with BBa_K10733034, the other with BBa_K1073035).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1073032
component: BBa_K1073028
sequence annotation: BBa_K1073028
sequence annotation: BBa_K1073032
sequence: https://synbiohub.org/public/igem/BBa_K1073035_sequence/1

BBa_K1073032
type: Component
persistent identity: component2377632
display id: component2377632
version: 1
top level: BBa_K1073035
definition: BBa_K1073032
access: public

BBa_K1073028
type: Component
persistent identity: component2377601
display id: component2377601
version: 1
top level: BBa_K1073035
definition: BBa_K1073028
access: public

BBa_K1073028
type: SequenceAnnotation
persistent identity: annotation2377601
display id: annotation2377601
version: 1
top level: BBa_K1073035
location: https://synbiohub.org/public/igem/BBa_K1073035/annotation2377601/range2377601/1
component: BBa_K1073028

https://synbiohub.org/public/igem/BBa_K1073035/annotation2377601/range2377601/1
type: Range
persistent identity: range2377601
display id: range2377601
version: 1
top level: BBa_K1073035
direction: direction/reverse
start: 1
end: 1938
orientation: inline

BBa_K1073032
type: SequenceAnnotation
persistent identity: annotation2377632
display id: annotation2377632
version: 1
top level: BBa_K1073035
location: https://synbiohub.org/public/igem/BBa_K1073035/annotation2377632/range2377632/1
component: BBa_K1073032

https://synbiohub.org/public/igem/BBa_K1073035/annotation2377632/range2377632/1
type: Range
persistent identity: range2377632
display id: range2377632
version: 1
top level: BBa_K1073035
direction: direction/reverse
start: 1947
end: 3657
orientation: inline

BBa_K1073028
type: ComponentDefinition
persistent identity: BBa K1073028
display id: BBa_K1073028
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible ampicillin resistance cassette + RhlR expression cassette
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: The biobricks for this part were taken from the 2013 distribution kit.
owned by: james
owned by: myers
mutable description: In complex with the inducer N-butyryl-HSL, the activator RhlR binds to the Rhl promoter and enables expression. This construct provides all the parts required to express the gene behind the rhl promoter. Only the inducer N-butyryl-HSL is missing, which usually is taken up from the media. By the regulation of the inducer's concentration in the media, the expression rate of the gene of interest (in this case ampicillin beta-lactamase) can be controlled.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1073014
component: BBa_B0015
component: BBa_K1073011
sequence annotation: BBa_K1073011
sequence annotation: BBa_B0015
sequence annotation: BBa_K1073014
sequence: https://synbiohub.org/public/igem/BBa_K1073028_sequence/1

BBa_K1073014
type: Component
persistent identity: component2374465
display id: component2374465
version: 1
top level: BBa_K1073028
definition: BBa_K1073014
access: public

BBa_B0015
type: Component
persistent identity: component2374455
display id: component2374455
version: 1
top level: BBa_K1073028
definition: BBa_B0015
access: public

BBa_K1073011
type: Component
persistent identity: component2374448
display id: component2374448
version: 1
top level: BBa_K1073028
definition: BBa_K1073011
access: public

BBa_K1073011
type: SequenceAnnotation
persistent identity: annotation2374448
display id: annotation2374448
version: 1
top level: BBa_K1073028
location: https://synbiohub.org/public/igem/BBa_K1073028/annotation2374448/range2374448/1
component: BBa_K1073011

https://synbiohub.org/public/igem/BBa_K1073028/annotation2374448/range2374448/1
type: Range
persistent identity: range2374448
display id: range2374448
version: 1
top level: BBa_K1073028
direction: direction/reverse
start: 1
end: 944
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2374455
display id: annotation2374455
version: 1
top level: BBa_K1073028
location: https://synbiohub.org/public/igem/BBa_K1073028/annotation2374455/range2374455/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1073028/annotation2374455/range2374455/1
type: Range
persistent identity: range2374455
display id: range2374455
version: 1
top level: BBa_K1073028
direction: direction/reverse
start: 953
end: 1081
orientation: inline

BBa_K1073014
type: SequenceAnnotation
persistent identity: annotation2374465
display id: annotation2374465
version: 1
top level: BBa_K1073028
location: https://synbiohub.org/public/igem/BBa_K1073028/annotation2374465/range2374465/1
component: BBa_K1073014

https://synbiohub.org/public/igem/BBa_K1073028/annotation2374465/range2374465/1
type: Range
persistent identity: range2374465
display id: range2374465
version: 1
top level: BBa_K1073028
direction: direction/reverse
start: 1090
end: 1938
orientation: inline

BBa_K1073032
type: ComponentDefinition
persistent identity: BBa K1073032
display id: BBa_K1073032
version: 1
was generated by: iGEM to SBOL conversion
description: Autoinducer synthase LasI + LVA with combined eforRed chromoprotein expression cassette
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: The biobricks for this part were derived from the 2013 distribution kit and the iGEM Team Uppsala.
owned by: james
owned by: myers
mutable description: This part is the basis for the expression of the Las auto inducer synthetase, which synthesizes LasI [N-(3-oxododecanoyl)-homoserine lactone]. LasI binds to the activator LasR. Together they activate the Las promoter. A constitutive expression of the chromoprotein eforRed is added to the construct in order to varify the expression of LasI synthetase. The construct has no promoter in front of the LasI synthetase gene. Therefore the user can choose the promoter himself (e.g. constitutive, induced, repressed, etc.)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K1073022
component: BBa_K1073018
sequence annotation: BBa_K1073018
sequence annotation: BBa_K1073022
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1073032_sequence/1

BBa_B0015
type: Component
persistent identity: component2354180
display id: component2354180
version: 1
top level: BBa_K1073032
definition: BBa_B0015
access: public

BBa_K1073022
type: Component
persistent identity: component2354173
display id: component2354173
version: 1
top level: BBa_K1073032
definition: BBa_K1073022
access: public

BBa_K1073018
type: Component
persistent identity: component2354165
display id: component2354165
version: 1
top level: BBa_K1073032
definition: BBa_K1073018
access: public

BBa_K1073018
type: SequenceAnnotation
persistent identity: annotation2354165
display id: annotation2354165
version: 1
top level: BBa_K1073032
location: https://synbiohub.org/public/igem/BBa_K1073032/annotation2354165/range2354165/1
component: BBa_K1073018

https://synbiohub.org/public/igem/BBa_K1073032/annotation2354165/range2354165/1
type: Range
persistent identity: range2354165
display id: range2354165
version: 1
top level: BBa_K1073032
direction: direction/reverse
start: 1
end: 823
orientation: inline

BBa_K1073022
type: SequenceAnnotation
persistent identity: annotation2354173
display id: annotation2354173
version: 1
top level: BBa_K1073032
location: https://synbiohub.org/public/igem/BBa_K1073032/annotation2354173/range2354173/1
component: BBa_K1073022

https://synbiohub.org/public/igem/BBa_K1073032/annotation2354173/range2354173/1
type: Range
persistent identity: range2354173
display id: range2354173
version: 1
top level: BBa_K1073032
direction: direction/reverse
start: 832
end: 1574
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2354180
display id: annotation2354180
version: 1
top level: BBa_K1073032
location: https://synbiohub.org/public/igem/BBa_K1073032/annotation2354180/range2354180/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1073032/annotation2354180/range2354180/1
type: Range
persistent identity: range2354180
display id: range2354180
version: 1
top level: BBa_K1073032
direction: direction/reverse
start: 1583
end: 1711
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1073011
type: ComponentDefinition
persistent identity: BBa K1073011
display id: BBa_K1073011
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible ampicillin resistance cassette (regulated by RhlR + N-buturyl-HSL)
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: This part is a combination of BBa_K1073001 and BBa_K1073000, which themselves were constructed from biobricks of the 2013 distribution kit.
owned by: james
owned by: myers
mutable description: The promoter for this beta-lactonase is induced by N-(butyryl)-homoserine lactone. Therefore the expression of the ampicillin beta-lactonase is inducable by Rhl Inducer N-(butyryl)-homoserine lactone.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1073000
component: BBa_K1073001
sequence annotation: BBa_K1073001
sequence annotation: BBa_K1073000
sequence: https://synbiohub.org/public/igem/BBa_K1073011_sequence/1

BBa_K1073000
type: Component
persistent identity: component2373103
display id: component2373103
version: 1
top level: BBa_K1073011
definition: ampR
access: public

BBa_K1073001
type: Component
persistent identity: component2373102
display id: component2373102
version: 1
top level: BBa_K1073011
definition: pRhl + RBS
access: public

BBa_K1073001
type: SequenceAnnotation
persistent identity: annotation2373102
display id: annotation2373102
version: 1
top level: BBa_K1073011
location: https://synbiohub.org/public/igem/BBa_K1073011/annotation2373102/range2373102/1
component: BBa_K1073001

https://synbiohub.org/public/igem/BBa_K1073011/annotation2373102/range2373102/1
type: Range
persistent identity: range2373102
display id: range2373102
version: 1
top level: BBa_K1073011
direction: direction/reverse
start: 1
end: 74
orientation: inline

BBa_K1073000
type: SequenceAnnotation
persistent identity: annotation2373103
display id: annotation2373103
version: 1
top level: BBa_K1073011
location: https://synbiohub.org/public/igem/BBa_K1073011/annotation2373103/range2373103/1
component: BBa_K1073000

https://synbiohub.org/public/igem/BBa_K1073011/annotation2373103/range2373103/1
type: Range
persistent identity: range2373103
display id: range2373103
version: 1
top level: BBa_K1073011
direction: direction/reverse
start: 81
end: 944
orientation: inline

BBa_K1073014
type: ComponentDefinition
persistent identity: BBa K1073014
display id: BBa_K1073014
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutively expressed transcription regulator RhlR + LVA
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: This part is a combination of the BBa_K1073006 and BBa_C0071.
owned by: james
owned by: myers
mutable description: The RhlR synthetase is constitutively expressed by a strong promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0071
component: BBa_K1073006
sequence annotation: BBa_K1073006
sequence annotation: BBa_C0071
sequence: https://synbiohub.org/public/igem/BBa_K1073014_sequence/1

BBa_C0071
type: Component
persistent identity: component2354041
display id: component2354041
version: 1
top level: BBa_K1073014
definition: rhlR
access: public

BBa_K1073006
type: Component
persistent identity: component2354037
display id: component2354037
version: 1
top level: BBa_K1073014
definition: BBa_K1073006
access: public

BBa_K1073006
type: SequenceAnnotation
persistent identity: annotation2354037
display id: annotation2354037
version: 1
top level: BBa_K1073014
location: https://synbiohub.org/public/igem/BBa_K1073014/annotation2354037/range2354037/1
component: BBa_K1073006

https://synbiohub.org/public/igem/BBa_K1073014/annotation2354037/range2354037/1
type: Range
persistent identity: range2354037
display id: range2354037
version: 1
top level: BBa_K1073014
direction: direction/reverse
start: 1
end: 56
orientation: inline

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation2354041
display id: annotation2354041
version: 1
top level: BBa_K1073014
location: https://synbiohub.org/public/igem/BBa_K1073014/annotation2354041/range2354041/1
component: BBa_C0071

https://synbiohub.org/public/igem/BBa_K1073014/annotation2354041/range2354041/1
type: Range
persistent identity: range2354041
display id: range2354041
version: 1
top level: BBa_K1073014
direction: direction/reverse
start: 63
end: 849
orientation: inline

BBa_K1073018
type: ComponentDefinition
persistent identity: BBa K1073018
display id: BBa_K1073018
version: 1
was generated by: iGEM to SBOL conversion
description: Autoinducer synthetase LasI ( producing N-3-oxododecanoyl-HSL) with RBS and double terminator
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: The part is a combination of BBa_B0032, BBa_C0078, and BBa_B0015, which were taken from the 2013 distribution kit.
owned by: james
owned by: myers
mutable description: To the lasI protein, which produces the chemical signal AI-1, a RBS and a double terminator was added to facilitate the construction of an expression system.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0078
component: BBa_B0032
component: BBa_B0015
sequence annotation: BBa_B0032
sequence annotation: BBa_C0078
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1073018_sequence/1

BBa_C0078
type: Component
persistent identity: component2354058
display id: component2354058
version: 1
top level: BBa_K1073018
definition: lasI
access: public

BBa_B0032
type: Component
persistent identity: component2354053
display id: component2354053
version: 1
top level: BBa_K1073018
definition: BBa_B0032
access: public

BBa_B0015
type: Component
persistent identity: component2354065
display id: component2354065
version: 1
top level: BBa_K1073018
definition: BBa_B0015
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2354053
display id: annotation2354053
version: 1
top level: BBa_K1073018
location: https://synbiohub.org/public/igem/BBa_K1073018/annotation2354053/range2354053/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1073018/annotation2354053/range2354053/1
type: Range
persistent identity: range2354053
display id: range2354053
version: 1
top level: BBa_K1073018
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation2354058
display id: annotation2354058
version: 1
top level: BBa_K1073018
location: https://synbiohub.org/public/igem/BBa_K1073018/annotation2354058/range2354058/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_K1073018/annotation2354058/range2354058/1
type: Range
persistent identity: range2354058
display id: range2354058
version: 1
top level: BBa_K1073018
direction: direction/reverse
start: 20
end: 686
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2354065
display id: annotation2354065
version: 1
top level: BBa_K1073018
location: https://synbiohub.org/public/igem/BBa_K1073018/annotation2354065/range2354065/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1073018/annotation2354065/range2354065/1
type: Range
persistent identity: range2354065
display id: range2354065
version: 1
top level: BBa_K1073018
direction: direction/reverse
start: 695
end: 823
orientation: inline

BBa_K1073022
type: ComponentDefinition
persistent identity: BBa K1073022
display id: BBa_K1073022
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutively expressed chromoprotein eforRed
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: This part is a combination of BBa_K1073006 and BBa_K592012, which were derived from the 2013 distribution kit and the iGEM Team Uppsala.
owned by: james
owned by: myers
mutable description: The chromoprotein eforRed is constitutively expressed and can be used for the marking of cells for easier differentiation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_K592012
component: BBa_K1073006
sequence annotation: BBa_K1073006
sequence annotation: BBa_K592012
sequence: https://synbiohub.org/public/igem/BBa_K1073022_sequence/1

BBa_K592012
type: Component
persistent identity: component2354079
display id: component2354079
version: 1
top level: BBa_K1073022
definition: eforRed
access: public

BBa_K1073006
type: Component
persistent identity: component2354077
display id: component2354077
version: 1
top level: BBa_K1073022
definition: BBa_K1073006
access: public

BBa_K1073006
type: SequenceAnnotation
persistent identity: annotation2354077
display id: annotation2354077
version: 1
top level: BBa_K1073022
location: https://synbiohub.org/public/igem/BBa_K1073022/annotation2354077/range2354077/1
component: BBa_K1073006

https://synbiohub.org/public/igem/BBa_K1073022/annotation2354077/range2354077/1
type: Range
persistent identity: range2354077
display id: range2354077
version: 1
top level: BBa_K1073022
direction: direction/reverse
start: 1
end: 56
orientation: inline

BBa_K592012
type: SequenceAnnotation
persistent identity: annotation2354079
display id: annotation2354079
version: 1
top level: BBa_K1073022
location: https://synbiohub.org/public/igem/BBa_K1073022/annotation2354079/range2354079/1
component: BBa_K592012

https://synbiohub.org/public/igem/BBa_K1073022/annotation2354079/range2354079/1
type: Range
persistent identity: range2354079
display id: range2354079
version: 1
top level: BBa_K1073022
direction: direction/reverse
start: 63
end: 743
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

ampR
type: ComponentDefinition
persistent identity: BBa K1073000
display id: BBa_K1073000
version: 1
was generated by: iGEM to SBOL conversion
description: ampicillin resistance
created: 2013-09-15T11:00:00Z
modified: 2016-01-28T01:50:42Z
mutable provenance: The part was derived from BBa_P1002 ampicillin resistance cassette. The sequence for the beta-lactamase was gained by PCR of the original BBa_P1002 with appropiate primers.
owned by: james
owned by: myers
mutable description: This sequence codes for a beta-lactamase, which degrades ampicillin. There is no RBS or other information attached, only the sequence for the enzyme plus prefix and suffix.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 4206
owner id: 15674
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The primer sequences can be found on iGEM Team Braunschweig's wiki.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1073000_sequence/1

pRhl + RBS
type: ComponentDefinition
persistent identity: BBa K1073001
display id: BBa_K1073001
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible promoter of the rhl quorum sensing system with RBS
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: Combined construct from BBa_R0071 and BBa_B0032, which were taken from the 2013 distribution kit.
owned by: james
owned by: myers
mutable description: This construct is derived from BBa_R0071 and B0032. It combines the rhl induced promoter with an RBS and can be used to clone it in front of a desired gene of interest, which shall be induced by rhl.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_R0071
component: BBa_B0032
sequence annotation: BBa_R0071
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K1073001_sequence/1

BBa_R0071
type: Component
persistent identity: component2346211
display id: component2346211
version: 1
top level: pRhl + RBS
definition: RhlR+C4
access: public

BBa_B0032
type: Component
persistent identity: component2346213
display id: component2346213
version: 1
top level: pRhl + RBS
definition: BBa_B0032
access: public

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2346211
display id: annotation2346211
version: 1
top level: pRhl + RBS
location: https://synbiohub.org/public/igem/BBa_K1073001/annotation2346211/range2346211/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_K1073001/annotation2346211/range2346211/1
type: Range
persistent identity: range2346211
display id: range2346211
version: 1
top level: pRhl + RBS
direction: direction/reverse
start: 1
end: 53
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2346213
display id: annotation2346213
version: 1
top level: pRhl + RBS
location: https://synbiohub.org/public/igem/BBa_K1073001/annotation2346213/range2346213/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1073001/annotation2346213/range2346213/1
type: Range
persistent identity: range2346213
display id: range2346213
version: 1
top level: pRhl + RBS
direction: direction/forward
start: 62
end: 74
orientation: inline

BBa_K1073006
type: ComponentDefinition
persistent identity: BBa K1073006
display id: BBa_K1073006
version: 1
was generated by: iGEM to SBOL conversion
description: strong constitutive promoter with RBS
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:01Z
mutable provenance: This part is a combination of BBa_J23106 and BBa_B0032, which were taken from 2013 distribution kit.
owned by: james
owned by: myers
mutable description: This part combines a constitutive promoter with an RBS and can be cloned in front of a gene of interest. It is a combination of BBa_J23106 and BBa_B0032.
discontinued: false
dominant: false
experience: experience/None
group u list: _1382_
m user id: 0
owner id: 15674
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0032
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K1073006_sequence/1

BBa_B0032
type: Component
persistent identity: component2352910
display id: component2352910
version: 1
top level: BBa_K1073006
definition: BBa_B0032
access: public

BBa_J23100
type: Component
persistent identity: component2352908
display id: component2352908
version: 1
top level: BBa_K1073006
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2352908
display id: annotation2352908
version: 1
top level: BBa_K1073006
location: https://synbiohub.org/public/igem/BBa_K1073006/annotation2352908/range2352908/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1073006/annotation2352908/range2352908/1
type: Range
persistent identity: range2352908
display id: range2352908
version: 1
top level: BBa_K1073006
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2352910
display id: annotation2352910
version: 1
top level: BBa_K1073006
location: https://synbiohub.org/public/igem/BBa_K1073006/annotation2352910/range2352910/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1073006/annotation2352910/range2352910/1
type: Range
persistent identity: range2352910
display id: range2352910
version: 1
top level: BBa_K1073006
direction: direction/forward
start: 44
end: 56
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

lasI
type: ComponentDefinition
persistent identity: BBa C0078
display id: BBa_C0078
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for PAI from Pseudomonas aeruginosa
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for lasI protein, which produces the chemical signal AI-1
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Chris Walsh (Fighting Darwins)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LasI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0078_sequence/1

LasI
type: SequenceAnnotation
persistent identity: annotation305970
display id: annotation305970
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1
type: Range
persistent identity: range305970
display id: range305970
version: 1
top level: lasI
direction: direction/forward
start: 1
end: 603
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306608
display id: annotation306608
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1
type: Range
persistent identity: range306608
display id: range306608
version: 1
top level: lasI
direction: direction/forward
start: 604
end: 636
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214003
display id: annotation2214003
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1
type: Range
persistent identity: range2214003
display id: range2214003
version: 1
top level: lasI
direction: direction/forward
start: 643
end: 667
orientation: inline

rhlR
type: ComponentDefinition
persistent identity: BBa C0071
display id: BBa_C0071
version: 1
was generated by: iGEM to SBOL conversion
description: rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: P. aeruginosa PA3477
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcriptional regulator, in complex with N-butyryl-HSL, RhlR binds to the Rhl promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: (1/28/04 Edit Jamboree) (0) No BB sites (1) ATG (2) TAATAA (3) Blast checks out (4) No codon changes (5) Maybe chassis compatability (check by experiment) (6) Codon usage not optimal (but looks OK) (7) ssrA tag added
star: true
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: rhlR
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0071_sequence/1

rhlR
type: SequenceAnnotation
persistent identity: annotation301192
display id: annotation301192
version: 1
top level: rhlR
location: https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
type: Range
persistent identity: range301192
display id: range301192
version: 1
top level: rhlR
direction: direction/forward
start: 1
end: 723
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306523
display id: annotation306523
version: 1
top level: rhlR
location: https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
type: Range
persistent identity: range306523
display id: range306523
version: 1
top level: rhlR
direction: direction/forward
start: 724
end: 756
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213995
display id: annotation2213995
version: 1
top level: rhlR
location: https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
type: Range
persistent identity: range2213995
display id: range2213995
version: 1
top level: rhlR
direction: direction/forward
start: 763
end: 787
orientation: inline

eforRed
type: ComponentDefinition
persistent identity: BBa K592012
display id: BBa_K592012
version: 1
was generated by: iGEM to SBOL conversion
description: eforRed, red chromoprotein
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:49Z
mutable provenance: coming soon
owned by: james
owned by: myers
mutable description: This chromoprotein eforRed naturally exhibits red color when expressed. The color is weaker than RFP, however. On agar plates and in liquid culture, the color is readily visible to naked eye in less than 24 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: coming soon
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: eforRed
sequence: https://synbiohub.org/public/igem/BBa_K592012_sequence/1

eforRed
type: SequenceAnnotation
persistent identity: annotation2131799
display id: annotation2131799
version: 1
top level: eforRed
location: https://synbiohub.org/public/igem/BBa_K592012/annotation2131799/range2131799/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K592012/annotation2131799/range2131799/1
type: Range
persistent identity: range2131799
display id: range2131799
version: 1
top level: eforRed
direction: direction/forward
start: 1
end: 681
orientation: inline

RhlR+C4
type: ComponentDefinition
persistent identity: BBa R0071
display id: BBa_R0071
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (RhlR & C4-HSL regulated)
created: 2004-01-24T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Pseudomonas aeruginosa rhlAB promoter
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. This is the natural sequence taken from Pseudomonas aeruginosa. Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The -10 and -35 sites are labeled as identified in [Pearson97]. The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
star: true
creator: Ronny Krashinsky
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RhlR
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0071_sequence/1

RhlR
type: SequenceAnnotation
persistent identity: annotation297102
display id: annotation297102
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
type: Range
persistent identity: range297102
display id: range297102
version: 1
top level: RhlR+C4
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation297103
display id: annotation297103
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
type: Range
persistent identity: range297103
display id: range297103
version: 1
top level: RhlR+C4
direction: direction/forward
start: 19
end: 24
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation297104
display id: annotation297104
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
type: Range
persistent identity: range297104
display id: range297104
version: 1
top level: RhlR+C4
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation297105
display id: annotation297105
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
type: Range
persistent identity: range297105
display id: range297105
version: 1
top level: RhlR+C4
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_K1073035_sequence/1
type: Sequence
persistent identity: BBa K1073035 sequence
display id: BBa_K1073035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagtcacacaggaaagtactagatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacaggaaagtactagatgatcgttcagatcggtcgtcgtgaagagttcga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073028_sequence/1
type: Sequence
persistent identity: BBa K1073028 sequence
display id: BBa_K1073028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagtcacacaggaaagtactagatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073032_sequence/1
type: Sequence
persistent identity: BBa K1073032 sequence
display id: BBa_K1073032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatgtcagtgattaagcaggtaatgaagaccaagttgcaccttgagggcactgtcaatggccatgattttacgatcgagggtaaaggtgaaggcaagccgtacgaagggttacagcacatgaaaatgacagtcaccaaaggcgcgcctctgccgttttccgttcatattcttacacctagccacatgtatggaagcaaaccgtttaataagtatccagcggatatcccagactaccacaaacagtcttttcccgaaggtatgtcttgggagcggtcgatgatttttgaagatggtggcgtatgcaccgccagtaatcactccagcataaacttgcaagagaactgtttcatctatgatgttaaatttcatggtgtgaacctgcctccggatgggcccgtaatgcaaaaaaccattgctggatgggagccgagcgtggaaacactgtacgtgcgtgacgggatgttaaaaagtgacactgcaatggtttttaaactgaaaggaggcggtcatcatcgtgttgatttcaaaacgacgtataaagccaaaaaacctgtcaagctgccagaatttcatttcgttgaacatcgcctggaactgaccaaacacgataaagatttcacaacttgggaccagcaggaggcagccgaaggccatttctcaccgctgccgaaggctctcccatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073011_sequence/1
type: Sequence
persistent identity: BBa K1073011 sequence
display id: BBa_K1073011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagtcacacaggaaagtactagatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073014_sequence/1
type: Sequence
persistent identity: BBa K1073014 sequence
display id: BBa_K1073014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073018_sequence/1
type: Sequence
persistent identity: BBa K1073018 sequence
display id: BBa_K1073018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073022_sequence/1
type: Sequence
persistent identity: BBa K1073022 sequence
display id: BBa_K1073022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatgtcagtgattaagcaggtaatgaagaccaagttgcaccttgagggcactgtcaatggccatgattttacgatcgagggtaaaggtgaaggcaagccgtacgaagggttacagcacatgaaaatgacagtcaccaaaggcgcgcctctgccgttttccgttcatattcttacacctagccacatgtatggaagcaaaccgtttaataagtatccagcggatatcccagactaccacaaacagtcttttcccgaaggtatgtcttgggagcggtcgatgatttttgaagatggtggcgtatgcaccgccagtaatcactccagcataaacttgcaagagaactgtttcatctatgatgttaaatttcatggtgtgaacctgcctccggatgggcccgtaatgcaaaaaaccattgctggatgggagccgagcgtggaaacactgtacgtgcgtgacgggatgttaaaaagtgacactgcaatggtttttaaactgaaaggaggcggtcatcatcgtgttgatttcaaaacgacgtataaagccaaaaaacctgtcaagctgccagaatttcatttcgttgaacatcgcctggaactgaccaaacacgataaagatttcacaacttgggaccagcaggaggcagccgaaggccatttctcaccgctgccgaaggctctccca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073000_sequence/1
type: Sequence
persistent identity: BBa K1073000 sequence
display id: BBa_K1073000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073001_sequence/1
type: Sequence
persistent identity: BBa K1073001 sequence
display id: BBa_K1073001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagtcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073006_sequence/1
type: Sequence
persistent identity: BBa K1073006 sequence
display id: BBa_K1073006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0078_sequence/1
type: Sequence
persistent identity: BBa C0078 sequence
display id: BBa_C0078_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0071_sequence/1
type: Sequence
persistent identity: BBa C0071 sequence
display id: BBa_C0071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592012_sequence/1
type: Sequence
persistent identity: BBa K592012 sequence
display id: BBa_K592012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcagtgattaagcaggtaatgaagaccaagttgcaccttgagggcactgtcaatggccatgattttacgatcgagggtaaaggtgaaggcaagccgtacgaagggttacagcacatgaaaatgacagtcaccaaaggcgcgcctctgccgttttccgttcatattcttacacctagccacatgtatggaagcaaaccgtttaataagtatccagcggatatcccagactaccacaaacagtcttttcccgaaggtatgtcttgggagcggtcgatgatttttgaagatggtggcgtatgcaccgccagtaatcactccagcataaacttgcaagagaactgtttcatctatgatgttaaatttcatggtgtgaacctgcctccggatgggcccgtaatgcaaaaaaccattgctggatgggagccgagcgtggaaacactgtacgtgcgtgacgggatgttaaaaagtgacactgcaatggtttttaaactgaaaggaggcggtcatcatcgtgttgatttcaaaacgacgtataaagccaaaaaacctgtcaagctgccagaatttcatttcgttgaacatcgcctggaactgaccaaacacgataaagatttcacaacttgggaccagcaggaggcagccgaaggccatttctcaccgctgccgaaggctctccca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0071_sequence/1
type: Sequence
persistent identity: BBa R0071 sequence
display id: BBa_R0071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z