LOCUS BBa_K1073036 7544 bp DNA linear 03-Jun-2024 DEFINITION test ACCESSION BBa_K1073036 VERSION BBa_K1073036.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=RhlR promoter 19..24 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start promoter 1..53 /label=BBa_R0071 RBS 62..74 /label=RBS-3\Weak RBS 68..71 /label=RBS misc_feature 62..74 /label=BBa_B0032 RBS 62..74 /label=BBa_B0032 promoter 1..74 /label=BBa_K1073001 CDS 81..944 /label=BBa_K1073000 misc_feature 1..944 /label=BBa_K1073011 stem_loop 964..1007 /label=stem_loop misc_feature 953..1032 /label=BBa_B0010 terminator 953..1032 /label=BBa_B0010 stem_loop 1048..1067 /label=T7 TE misc_feature 1074..1074 /label=stop polyA_site 1068..1081 /label=polya misc_feature 1041..1081 /label=BBa_B0012 terminator 1041..1081 /label=BBa_B0012 terminator 953..1081 /label=BBa_B0015 promoter 1090..1124 /label=BBa_J23100 RBS 1133..1145 /label=RBS-3\Weak RBS 1139..1142 /label=RBS misc_feature 1133..1145 /label=BBa_B0032 RBS 1133..1145 /label=BBa_B0032 promoter 1090..1145 /label=BBa_K1073006 CDS 1152..1874 /label=rhlR misc_feature 1875..1907 /label=LVA misc_feature 1914..1938 /label=Help:Barcodes CDS 1152..1938 /label=BBa_C0071 misc_feature 1090..1938 /label=BBa_K1073014 misc_feature 1..1938 /label=BBa_K1073028 RBS 1947..1959 /label=RBS-3\Weak RBS 1953..1956 /label=RBS misc_feature 1947..1959 /label=BBa_B0032 RBS 1947..1959 /label=BBa_B0032 CDS 1966..2568 /label=LasI misc_feature 2569..2601 /label=LVA misc_feature 2608..2632 /label=Help:Barcodes CDS 1966..2632 /label=BBa_C0078 stem_loop 2652..2695 /label=stem_loop misc_feature 2641..2720 /label=BBa_B0010 terminator 2641..2720 /label=BBa_B0010 stem_loop 2736..2755 /label=T7 TE misc_feature 2762..2762 /label=stop polyA_site 2756..2769 /label=polya misc_feature 2729..2769 /label=BBa_B0012 terminator 2729..2769 /label=BBa_B0012 terminator 2641..2769 /label=BBa_B0015 misc_feature 1947..2769 /label=BBa_K1073018 promoter 2778..2812 /label=BBa_J23100 RBS 2821..2833 /label=RBS-3\Weak RBS 2827..2830 /label=RBS misc_feature 2821..2833 /label=BBa_B0032 RBS 2821..2833 /label=BBa_B0032 promoter 2778..2833 /label=BBa_K1073006 CDS 2840..3520 /label=eforRed CDS 2840..3520 /label=BBa_K592012 misc_feature 2778..3520 /label=BBa_K1073022 stem_loop 3540..3583 /label=stem_loop misc_feature 3529..3608 /label=BBa_B0010 terminator 3529..3608 /label=BBa_B0010 stem_loop 3624..3643 /label=T7 TE misc_feature 3650..3650 /label=stop polyA_site 3644..3657 /label=polya misc_feature 3617..3657 /label=BBa_B0012 terminator 3617..3657 /label=BBa_B0012 terminator 3529..3657 /label=BBa_B0015 misc_feature 1947..3657 /label=BBa_K1073032 misc_feature 1..3657 /label=BBa_K1073035 stem_loop 3677..3720 /label=stem_loop misc_feature 3666..3745 /label=BBa_B0010 terminator 3666..3745 /label=BBa_B0010 stem_loop 3761..3780 /label=T7 TE misc_feature 3787..3787 /label=stop polyA_site 3781..3794 /label=polya misc_feature 3754..3794 /label=BBa_B0012 terminator 3754..3794 /label=BBa_B0012 terminator 3666..3794 /label=BBa_B0015 misc_feature 3888..3893 /label=-35 Region misc_feature 3877..3895 /label=LasR Binding Site misc_feature 3915..3920 /label= -10 Region misc_feature 3927..3927 /label=Primary Transcriptional Start promoter 3803..3928 /label=BBa_K091117 RBS 3937..3949 /label=RBS-3\Weak RBS 3943..3946 /label=RBS misc_feature 3937..3949 /label=BBa_B0032 RBS 3937..3949 /label=BBa_B0032 promoter 3803..3949 /label=BBa_K1073003 CDS 3956..4819 /label=BBa_K1073000 misc_feature 3803..4819 /label=BBa_K1073013 stem_loop 4839..4882 /label=stem_loop misc_feature 4828..4907 /label=BBa_B0010 terminator 4828..4907 /label=BBa_B0010 stem_loop 4923..4942 /label=T7 TE misc_feature 4949..4949 /label=stop polyA_site 4943..4956 /label=polya misc_feature 4916..4956 /label=BBa_B0012 terminator 4916..4956 /label=BBa_B0012 terminator 4828..4956 /label=BBa_B0015 promoter 4965..4999 /label=BBa_J23100 RBS 5008..5020 /label=RBS-3\Weak RBS 5014..5017 /label=RBS misc_feature 5008..5020 /label=BBa_B0032 RBS 5008..5020 /label=BBa_B0032 promoter 4965..5020 /label=BBa_K1073006 CDS 5027..5743 /label=lasR misc_feature 5744..5776 /label=LVA misc_feature 5506..5506 /label=G misc_feature 5783..5807 /label=Help:Barcodes CDS 5027..5807 /label=BBa_C0079 misc_feature 4965..5807 /label=BBa_K1073016 misc_feature 3803..5807 /label=BBa_K1073030 RBS 5816..5828 /label=RBS-3\Weak RBS 5822..5825 /label=RBS misc_feature 5816..5828 /label=BBa_B0032 RBS 5816..5828 /label=BBa_B0032 CDS 5835..6437 /label=rhlL misc_feature 6438..6470 /label=LVA misc_feature 6477..6501 /label=Help:Barcodes CDS 5835..6501 /label=BBa_C0070 stem_loop 6521..6564 /label=stem_loop misc_feature 6510..6589 /label=BBa_B0010 terminator 6510..6589 /label=BBa_B0010 stem_loop 6605..6624 /label=T7 TE misc_feature 6631..6631 /label=stop polyA_site 6625..6638 /label=polya misc_feature 6598..6638 /label=BBa_B0012 terminator 6598..6638 /label=BBa_B0012 terminator 6510..6638 /label=BBa_B0015 misc_feature 5816..6638 /label=BBa_K1073019 promoter 6647..6681 /label=BBa_J23100 RBS 6690..6702 /label=RBS-3\Weak RBS 6696..6699 /label=RBS misc_feature 6690..6702 /label=BBa_B0032 RBS 6690..6702 /label=BBa_B0032 promoter 6647..6702 /label=BBa_K1073006 CDS 6709..7407 /label=aeBlue CDS 6709..7407 /label=BBa_K864401 misc_feature 6647..7407 /label=BBa_K1073020 stem_loop 7427..7470 /label=stem_loop misc_feature 7416..7495 /label=BBa_B0010 terminator 7416..7495 /label=BBa_B0010 stem_loop 7511..7530 /label=T7 TE misc_feature 7537..7537 /label=stop polyA_site 7531..7544 /label=polya misc_feature 7504..7544 /label=BBa_B0012 terminator 7504..7544 /label=BBa_B0012 terminator 7416..7544 /label=BBa_B0015 misc_feature 5816..7544 /label=BBa_K1073033 misc_feature 3666..7544 /label=BBa_K1073034 ORIGIN 1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga 61 gtcacacagg aaagtactag atgagtattc aacatttccg tgtcgccctt attccctttt 121 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 181 ccgaagatca gttgggtgca cgtgtgggtt acatcgaact ggacctcaac agcggtaaga 241 ttcttgagag ttttcgcccc gaagaacgtt tcccaatgat gagcactttt aaagttctgc 301 tctgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 361 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggacg 421 gcatgacagt acgcgaatta tgcagcgctg ccataaccat gagtgataac acggcggcca 481 acttacttct gacaacgatc ggaggaccga aggagcttac cgcttttttg cacaacatgg 541 gtgatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 601 acgagcgtga caccacgatg cctgtagcta tggcaacaac gttgcgcaaa ctcttaactg 661 gcgaacttct tactctcgct tcccggcaac aattaataga ctggatggag gcggataaag 721 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 781 gagccggtga gcgtgggtcc cgcggtatta ttgcagccct ggggccagat ggtaagccct 841 cccgtatcgt agttatctac acgacgggga gccaggcaac tatggacgaa cgtaatcgcc 901 agatcgctga gataggtgcc tccctgatta agcattggta ataatactag agccaggcat 961 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 1021 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 1081 atactagagt tgacggctag ctcagtccta ggtacagtgc tagctactag agtcacacag 1141 gaaagtacta gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg 1201 agatgcagcc gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc 1261 gcctgggctt cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga 1321 agaccgaggt ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact 1381 acggggccgt ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga 1441 gcgacagcct gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct 1501 gtgtcggcgc gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg 1561 cgcgcgacca gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt 1621 gcatgatcga gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca 1681 acccggtctg cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga 1741 gttccgggga aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca 1801 agaacatcca gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg 1861 cgctgggtct catcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat 1921 agtgctagtg tagatcacta ctagagtcac acaggaaagt actagatgat cgttcagatc 1981 ggtcgtcgtg aagagttcga caaaaaactg ctgggtgaaa tgcacaaact gcgtgctcag 2041 gttttcaaag aacgtaaagg ttgggacgtt tccgttatcg acgaaatgga aatcgacggt 2101 tacgacgctc tgtccccgta ctacatgctg atccaggaag acaccccgga agctcaggtt 2161 ttcggttgct ggcgtatctt cgacaccacc ggtccgtaca tgctgaaaaa caccttcccg 2221 gaactgctgc acggtaaaga agctccgtgc tccccgcaca tctgggaact gtcccgtttc 2281 gctatcaact ccggtcagaa aggttccctg ggtttctccg actgcaccct ggaagctatg 2341 cgtgctctgg ctcgttactc cttgcagaac gacatccaga ccctggttac cgttaccacc 2401 gttggtgttg aaaaaatgat gatccgtgct ggtctggacg tttcccgttt cggtccgcac 2461 ctgaaaatcg gtatcgaacg tgctgttgct ctgcgtatcg aactgaacgc taaaacccag 2521 atcgctctgt acggtggtgt tctggttgaa cagcgtctgg ctgtttccgc tgctaacgac 2581 gaaaactacg ctctggttgc ttaataactc tgatagtgct agtgtagatc tctactagag 2641 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 2701 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 2761 gcgtttatat actagagttg acggctagct cagtcctagg tacagtgcta gctactagag 2821 tcacacagga aagtactaga tgtcagtgat taagcaggta atgaagacca agttgcacct 2881 tgagggcact gtcaatggcc atgattttac gatcgagggt aaaggtgaag gcaagccgta 2941 cgaagggtta cagcacatga aaatgacagt caccaaaggc gcgcctctgc cgttttccgt 3001 tcatattctt acacctagcc acatgtatgg aagcaaaccg tttaataagt atccagcgga 3061 tatcccagac taccacaaac agtcttttcc cgaaggtatg tcttgggagc ggtcgatgat 3121 ttttgaagat ggtggcgtat gcaccgccag taatcactcc agcataaact tgcaagagaa 3181 ctgtttcatc tatgatgtta aatttcatgg tgtgaacctg cctccggatg ggcccgtaat 3241 gcaaaaaacc attgctggat gggagccgag cgtggaaaca ctgtacgtgc gtgacgggat 3301 gttaaaaagt gacactgcaa tggtttttaa actgaaagga ggcggtcatc atcgtgttga 3361 tttcaaaacg acgtataaag ccaaaaaacc tgtcaagctg ccagaatttc atttcgttga 3421 acatcgcctg gaactgacca aacacgataa agatttcaca acttgggacc agcaggaggc 3481 agccgaaggc catttctcac cgctgccgaa ggctctccca tactagagcc aggcatcaaa 3541 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 3601 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 3661 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 3721 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 3781 tttctgcgtt tatatactag agtgttctcg tgtgaagcca ttgctctgat cttttggacg 3841 tttcttcgag cctagcaagg gtccgggttc accgaaatct atctcatttg ctagttataa 3901 aattatgaaa tttgtataaa ttcttcagta ctagagtcac acaggaaagt actagatgag 3961 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 4021 tgctcaccca gaaacgctgg tgaaagtaaa agatgccgaa gatcagttgg gtgcacgtgt 4081 gggttacatc gaactggacc tcaacagcgg taagattctt gagagttttc gccccgaaga 4141 acgtttccca atgatgagca cttttaaagt tctgctctgt ggcgcggtat tatcccgtat 4201 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 4261 gtactcacca gtcacagaaa agcatcttac ggacggcatg acagtacgcg aattatgcag 4321 cgctgccata accatgagtg ataacacggc ggccaactta cttctgacaa cgatcggagg 4381 accgaaggag cttaccgctt ttttgcacaa catgggtgat catgtaactc gccttgatcg 4441 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 4501 agctatggca acaacgttgc gcaaactctt aactggcgaa cttcttactc tcgcttcccg 4561 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 4621 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtcccgcgg 4681 tattattgca gccctggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 4741 ggggagccag gcaactatgg acgaacgtaa tcgccagatc gctgagatag gtgcctccct 4801 gattaagcat tggtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 4861 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 4921 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagttgacg gctagctcag 4981 tcctaggtac agtgctagct actagagtca cacaggaaag tactagatgg ccttggttga 5041 cggttttctt gagctggaac gctcaagtgg aaaattggag tggagcgcca tcctccagaa 5101 gatggcgagc gaccttggat tctcgaagat cctgttcggc ctgttgccta aggacagcca 5161 ggactacgag aacgccttca tcgtcggcaa ctacccggcc gcctggcgcg agcattacga 5221 ccgggctggc tacgcgcggg tcgacccgac ggtcagtcac tgtacccaga gcgtactgcc 5281 gattttctgg gaaccgtcca tctaccagac gcgaaagcag cacgagttct tcgaggaagc 5341 ctcggccgcc ggcctggtgt atgggctgac catgccgctg catggtgctc gcggcgaact 5401 cggcgcgctg agcctcagcg tggaagcgga aaaccgggcc gaggccaacc gtttcataga 5461 gtcggtcctg ccgaccctgt ggatgctcaa ggactacgca ctgcaaagcg gtgccggact 5521 ggccttcgaa catccggtca gcaaaccggt ggttctgacc agccgggaga aggaagtgtt 5581 gcagtggtgc gccatcggca agaccagttg ggagatatcg gttatctgca actgctcgga 5641 agccaatgtg aacttccata tgggaaatat tcggcggaag ttcggtgtga cctcccgccg 5701 cgtagcggcc attatggccg ttaatttggg tcttattact ctcgctgcaa acgacgaaaa 5761 ctacgcttta gtagcttaat aacactgata gtgctagtgt agatcactac tagagtcaca 5821 caggaaagta ctagatgatc gaactgctgt ccgaatccct ggaaggtctg tccgctgcta 5881 tgatcgctga actgggtcgt taccgtcacc aggttttcat cgaaaaactg ggttgggacg 5941 ttgtttccac ctcccgtgtt cgtgaccagg agttcgacca gttcgaccac ccgcagaccc 6001 gttacatcgt tgctatgtcc cgtcagggta tctgcggttg cgctcgtctg ctgccgacca 6061 ccgacgctta cctgctgaaa gacgttttcg cttacctgtg ctccgaaacc ccgccgtccg 6121 acccgtccgt ttgggaactg tcccgttacg ctgcttccgc tgctgacgac ccgcagctgg 6181 ctatgaaaat cttctggtcc tccctccagt gcgcttggta cctgggtgct tcctccgttg 6241 ttgctgttac caccaccgct atggaacgtt acttcgttcg taacggtgtt atcctccagc 6301 gtctgggtcc gccgcagaaa gttaaaggtg aaaccctggt tgctatctcc ttcccggctt 6361 accaggaacg tggtctggaa atgctgctgc gttaccaccc ggaatggctc cagggtgttc 6421 cgctgtccat ggctgttgct gcaaacgacg aaaactacgc tttagtagct taataactct 6481 gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc 6541 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 6601 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttga cggctagctc 6661 agtcctaggt acagtgctag ctactagagt cacacaggaa agtactagat ggcttcactg 6721 gttaaaaaag acatgtgcat caaaatgacg atggaaggaa cagtaaacgg tcaccatttc 6781 aagtgtgtag gagaaggcga aggcaaacca tttgaaggga cccaggtgga aaagatacgc 6841 atcactgaag gtgggccctt accatttgcg tatgatattt tggccccttg ttgcatgtat 6901 ggcagtaaaa ccttcattaa gcatgtgtcg ggtattccgg attactttaa ggagtctttt 6961 cctgagggct ttacctggga aagaacacaa atcttcgagg atggcggcta tctcaccata 7021 caccaggaca cgagccttca gggtaataat tttattttca aagttaatgt catcggtgcc 7081 aacttccctg caaacggtcc cgtgatgcag aaaaaaacag ctggatggga accgtgcgtt 7141 gagatgcttt atccgcggga cggcgtcctg tgtggtcaga gcctgatggc cctgaaatgc 7201 actgatggca atcatctgac gtcccacctg cgcactacct atcgttctcg caagccatcc 7261 aatgcagtta acatgccgga atttcatttt ggggatcatc gcattgagat tttgaaagct 7321 gaacaaggta aattttatga acaatacgag tcagcggtgg cccgttactg tgaggcggca 7381 ccgagtaaat tagggcatca ctaataatac tagagccagg catcaaataa aacgaaaggc 7441 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 7501 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //