BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K1074006 1 BBa_K1074006 Pgrac+RBS+SamyQ+TD1+GFP 2013-09-08T11:00:00Z 2015-06-08T02:56:46Z synthetic This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins via a modular PCR or standard cut/ligation method. false false _1383_ 4206 16001 9 It's complicated true The transdermal-enhancing activity of the TD1 is sequence specific and dose dependent. false Changlong Zhao component2362105 1 BBa_K1074014 component2362103 1 BBa_K1074012 component2362108 1 BBa_E0040 component2362106 1 BBa_K1074000 component2362104 1 BBa_K1074015 annotation2362105 1 BBa_K1074014 range2362105 1 201 293 annotation2362104 1 BBa_K1074015 range2362104 1 179 194 annotation2362103 1 BBa_K1074012 range2362103 1 1 170 annotation2362106 1 BBa_K1074000 range2362106 1 302 334 annotation2362108 1 BBa_E0040 range2362108 1 341 1060 BBa_K1074012 1 BBa_K1074012 Pgrac promoter 2013-09-15T11:00:00Z 2015-05-08T01:09:02Z From plasmid PHT43 Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG,While the background level of expression of these expression cassettes is very low in the absence of the inducer, an induction factor of about 1,300 was measured using the bgaB reporter gene (Phan et al.,2005). The amount of recombinant protein produced after addition of IPTG may represent 10 and 13%, respectively, of the total cellular protein (demonstrated when fusing the htpG and pbpE genes to the groE promoter; Phan et al., 2005). false false _1383_ 0 16001 9 Not in stock false NO false Changlong Zhao BBa_K1074015 1 BBa_K1074015 RBS for Pgrac 2013-09-24T11:00:00Z 2015-05-08T01:09:02Z From recombinant vector PHT43. An efficient RBS for promoter Pgrac(BBa_K1074012) in Bacillus subtilis. We used it in all of our gene circuits containing promoter Pgrac. false false _1383_ 0 16001 9 Not in stock false synthetise with promoter Pgrac as a whole! false Changlong Zhao BBa_K1074000 1 BBa_K1074000 TD1, Transdermal peptide 2013-09-08T11:00:00Z 2015-05-08T01:09:02Z synthetic TD1 is a short synthetic peptide(ACSSSPSKHCG)identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin. false false _1383_ 0 16001 9 It's complicated true The transdermal-enhancing activity of the peptide was sequence specific. false Changlong Zhao BBa_K1074014 1 BBa_K1074014 SamyQ 2013-09-16T11:00:00Z 2015-05-08T01:09:02Z From recombinant plasmid PHT43. SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in Bacillus subtilis WB800N. Fuse it with the recombinant proteins to obtain the secretion of recombinant proteins in Bacillus subtilis. false false _1383_ 0 16001 9 Not in stock false NO false Changlong Zhao BBa_K1074000_sequence 1 gcttgttcttcttccccatctaagcattgtggt BBa_K1074006_sequence 1 ggtaccagctattgtaacataatcggtacgggggtgaaaaagctaacggaaaagggagcggaaaagaatgatgtaagcgtgaaaaattttttatcttatcacttgaaattggaagggagattctttattataagaattgtggaattgtgagcggataacaattcccaatttactagagaaaggaggaaggatcatactagatgattcaaaaacgaaagcggacagtttcgttcagacttgtgcttatgtgcacgctgttatttgtcagtttgccgattacaaaaacatcagcctactagaggcttgttcttcttccccatctaagcattgtggttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1074012_sequence 1 ggtaccagctattgtaacataatcggtacgggggtgaaaaagctaacggaaaagggagcggaaaagaatgatgtaagcgtgaaaaattttttatcttatcacttgaaattggaagggagattctttattataagaattgtggaattgtgagcggataacaattcccaatt BBa_K1074014_sequence 1 atgattcaaaaacgaaagcggacagtttcgttcagacttgtgcttatgtgcacgctgttatttgtcagtttgccgattacaaaaacatcagcc BBa_K1074015_sequence 1 aaaggaggaaggatca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z