BBa_K1074007
1
BBa_K1074007
Pctc+RBS+amilCP
2013-09-10T11:00:00Z
2015-05-08T01:09:02Z
2013 iGEM and Lab distribution
Promoter ctc is a sigma factor B-dependent promoter found in B. subtilis. In B. subtilis endogenous sigma factor B is activated under mild stress.amilCP, blue chromoprotein,naturally exhibits strong color when expressed.We assemble this gene circuit to get a visual signal when B.subtilis grow to the post-log phase.
false
false
_1383_
0
16001
9
It's complicated
false
NO
false
Changlong Zhao
component2362111
1
BBa_K143010
component2362112
1
BBa_K1074016
component2362114
1
BBa_K592009
annotation2362112
1
BBa_K1074016
range2362112
1
65
82
annotation2362114
1
BBa_K592009
range2362114
1
89
757
annotation2362111
1
BBa_K143010
range2362111
1
1
56
BBa_K592009
1
amilCP
amilCP, blue chromoprotein
2011-09-17T11:00:00Z
2015-05-08T01:12:48Z
Acropora millepora
Released HQ 2013
This chromoprotein, amilCP, naturally exhibits very strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. This DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of one illegal internal restriction site (EcoRI).
false
false
_763_
0
7929
9
In stock
true
Illegal internal restriction site had to be removed (EcoRI).
false
Lei Sun
annotation2131628
1
amilCP
range2131628
1
1
666
BBa_K1074016
1
BBa_K1074016
RBS for Pctc
2013-09-24T11:00:00Z
2015-05-08T01:09:02Z
From the genome of Bacillus subtilis. synthesized by Sangon Biotech.
This is an efficient RBS for promoter Pctc(BBa_K143010) in Bacillus subtilis. It's also an universal RBS for promoters in Bacillus subtilis. We also used it with the promoter PsdpRI(BBa_K1074013) in our project.
false
false
_1383_
0
16001
9
Not in stock
false
synthetise with promoter Pgrac as a whole!
false
Changlong Zhao
BBa_K143010
1
Pctc
Promoter ctc for B. subtilis
2008-09-10T11:00:00Z
2015-05-08T01:10:23Z
The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers <cite>2</cite><cite>3</cite>. This sequence was then synthesised by Geneart.
Promoter ctc is a sigma factor B dependent promoter found in ''B.subtilis''. In ''B.subtilis'' endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol<cite>1</cite>. The promoter ctc has been used previously as a read out for the activation of sigma factor B <cite>2</cite>.
*The context with which we used the promoter ctc, was to take blue light as an input and give '''Polymerase Per Second'''(PoPS) as an output. To do this the other potential inputs need to be carefully controlled so that only blue light activated the sigma B and gives a PoPS output. In order to get sufficient sigma B activation by blue light the light receptor YtvA, part...., needs to be over expressed in ''B.subtilis'' <cite>3</cite>.
===Reference===
<biblio>
#1 pmid=16267279
#2 pmid=3100810
#3 pmid=17575448
</biblio>
false
false
_199_
0
2090
9
Not in stock
false
Biobrick standard was applied to the promoter ctc sequence.
false
James Chappell
annotation1975701
1
Sigma B -35
range1975701
1
21
28
annotation1975700
1
Sigma B -10
range1975700
1
41
46
BBa_K1074016_sequence
1
aaaggtggtgaatactag
BBa_K1074007_sequence
1
cagctaaaccatttttcgaggtttaaatccttatcgttatgggtattgtttgtaattactagagaaaggtggtgaatactagtactagatgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataa
BBa_K592009_sequence
1
atgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataa
BBa_K143010_sequence
1
cagctaaaccatttttcgaggtttaaatccttatcgttatgggtattgtttgtaat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z