BBa_K1074009 1 BBa_K1074009 SdpA,SdpB,SpbC 2013-09-15T11:00:00Z 2015-05-08T01:09:02Z From the genome of Bacillus subtilis (strain 168) Killing factor of Bacillus subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation. Its maturation is dependent on SdpA and SdpB. Also functions as a ligand, being able to bind to SdpI thereby triggering a signal transduction cascade that protects the cell against the toxic effects of its own SdpC. We use this killing factor to build a kill switch for Bacillus subtilis. false false _1383_ 0 16001 9 Not in stock false With restriction sites of Spe I and Pst I in the sequence. false Changlong Zhao BBa_K1074012 1 BBa_K1074012 Pgrac promoter 2013-09-15T11:00:00Z 2015-05-08T01:09:02Z From plasmid PHT43 Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG,While the background level of expression of these expression cassettes is very low in the absence of the inducer, an induction factor of about 1,300 was measured using the bgaB reporter gene (Phan et al.,2005). The amount of recombinant protein produced after addition of IPTG may represent 10 and 13%, respectively, of the total cellular protein (demonstrated when fusing the htpG and pbpE genes to the groE promoter; Phan et al., 2005). false false _1383_ 0 16001 9 Not in stock false NO false Changlong Zhao BBa_K1074015 1 BBa_K1074015 RBS for Pgrac 2013-09-24T11:00:00Z 2015-05-08T01:09:02Z From recombinant vector PHT43. An efficient RBS for promoter Pgrac(BBa_K1074012) in Bacillus subtilis. We used it in all of our gene circuits containing promoter Pgrac. false false _1383_ 0 16001 9 Not in stock false synthetise with promoter Pgrac as a whole! false Changlong Zhao BBa_K1074010 1 BBa_K1074010 Pgrac+RBS+SDP 2013-09-24T11:00:00Z 2015-05-08T01:09:02Z Promoter Pgrac and RBS are synthesized via PCR, SDP operon is from genome of Bacillus subtilis Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of Bacillus subtilis. We put the SDP operon under the strong Regulated promoter Pgrac(BBa_K1074012) to see whether overexpression of spbc can induce the suicide of engineered Bacillus subtilis. false false _1383_ 0 16001 9 Not in stock false no false Changlong Zhao component2362123 1 BBa_K1074015 component2362124 1 BBa_K1074009 component2362122 1 BBa_K1074012 annotation2362124 1 BBa_K1074009 range2362124 1 201 2299 annotation2362122 1 BBa_K1074012 range2362122 1 1 170 annotation2362123 1 BBa_K1074015 range2362123 1 179 194 BBa_K1074010_sequence 1 ggtaccagctattgtaacataatcggtacgggggtgaaaaagctaacggaaaagggagcggaaaagaatgatgtaagcgtgaaaaattttttatcttatcacttgaaattggaagggagattctttattataagaattgtggaattgtgagcggataacaattcccaatttactagagaaaggaggaaggatcatactagatgactatatgttttctattattttcttcttattactttagcaatatttcacctcagaatccactgttcaaaaaaaattttttgcaacaattgtctccccaaggctttggcttttatagtaaaagccctacagaagaaaacatttcatttcacacaaaagaaaatttaaagttacctaatgcacttcccaataatttttttgggataaaaagagaaggaagagttcaggcaatagaattaggcaaaattgtagagaatatcgatccaaagaattggaaaacttgtgaaaacaacaactcctgcacaaatttagagaaacaaataaagcctattaaggttataaaaaatgaagattatatacatcttagcaaaggagaatacctaatatatcgccaaaaaccactctcatggtattggatagactttaagcaaactacctcttttgaaagaaaggtgctaaaaataaaaatagtatgaagatattaaatagtttagaaggttatattgacacctataatccatggaaaaatacatatgcactttttagaagtttacttggtttctcaacattactagtactattattcaatagtactgatattttatttagttatagtgcaaataatgtcacatgtgaaaatgtctatatccctaccgctttttgttttgctaaagaatatagtatcaattttgagattataagatacttaatgatttttatattaaccttagtggttatagggtggagacctagatttaccggtttatttcactggtatatttgctatagtattcaaacttcagctttaactatcgatggtggagagcaaattgcaactgttctttcttttcttatattacctgttacattattagattcaaggcgaaatcattggaatataaagaaaaacaataatgaatctttcacaaagaagacagtattgttttatataatgacaataattaaaattcaagtttttatcatttatttaaacgcagctttagagcgattgaaaaataaagagtgggcagaaggaacagcaatttactatttcttttctgatccggtgtttggattacctgaatatcaacttaacttaatgaatccactacttgaaagcaattttattgttgtcatcacttggttagtaactatttttgagttgttcttagcagcaagcataatttcaaatatcagaataaagagaattgcccttgttttgggaatattatttcatattgggataatattcagcattggtattgtaagttttggcttgatcatgatatcagcattaattatatatctgcatcctgtacaacaaaatatcactatgaattggtgttctcctttatttaaatatatatatgtaaaaggaaagagaaatttcaaaagaataggaggtgaatcagtcaagtttcttacaaaattgtttcatagctaacatttagataatggagaaataacttaatggaggtataataatttgaaaagtaaattacttaggctattgattgtttccatggtaacgatattggttttttcattagtaggactctctaaggagtcaagtacatctgctaaagaaaaccatacattttctggagaagattactttagaggacttttatttggacaaggggaagttggtaaattaatttcaaacgatttggaccctaaactcgtaaaagaggcaaatagtacagaaggtaaaaagttagtaaatgatgtagtcaaatttataaaaaaagatcaaccacaatatatggatgaattgaaacaatcgattgacagcaaagaccctaaaaaactcattgaaaatatgaccaaagcagaccaacttatccaaaaatatgctaagaaaaatgaaaacgtaaaatactcttctaataaagttactccatcttgtgggctttatgccgtctgtgtagcagctggatatttatatgttgtgggcgttaacgcagttgcattacaaacggctgccgcagtaacaactgcagtgtggaaatacgttgccaaatattcctcttcagcttctaataattctgatttagaagcggctgctgcaaaaaccctaaaattgattcatcaataa BBa_K1074009_sequence 1 atgactatatgttttctattattttcttcttattactttagcaatatttcacctcagaatccactgttcaaaaaaaattttttgcaacaattgtctccccaaggctttggcttttatagtaaaagccctacagaagaaaacatttcatttcacacaaaagaaaatttaaagttacctaatgcacttcccaataatttttttgggataaaaagagaaggaagagttcaggcaatagaattaggcaaaattgtagagaatatcgatccaaagaattggaaaacttgtgaaaacaacaactcctgcacaaatttagagaaacaaataaagcctattaaggttataaaaaatgaagattatatacatcttagcaaaggagaatacctaatatatcgccaaaaaccactctcatggtattggatagactttaagcaaactacctcttttgaaagaaaggtgctaaaaataaaaatagtatgaagatattaaatagtttagaaggttatattgacacctataatccatggaaaaatacatatgcactttttagaagtttacttggtttctcaacattactagtactattattcaatagtactgatattttatttagttatagtgcaaataatgtcacatgtgaaaatgtctatatccctaccgctttttgttttgctaaagaatatagtatcaattttgagattataagatacttaatgatttttatattaaccttagtggttatagggtggagacctagatttaccggtttatttcactggtatatttgctatagtattcaaacttcagctttaactatcgatggtggagagcaaattgcaactgttctttcttttcttatattacctgttacattattagattcaaggcgaaatcattggaatataaagaaaaacaataatgaatctttcacaaagaagacagtattgttttatataatgacaataattaaaattcaagtttttatcatttatttaaacgcagctttagagcgattgaaaaataaagagtgggcagaaggaacagcaatttactatttcttttctgatccggtgtttggattacctgaatatcaacttaacttaatgaatccactacttgaaagcaattttattgttgtcatcacttggttagtaactatttttgagttgttcttagcagcaagcataatttcaaatatcagaataaagagaattgcccttgttttgggaatattatttcatattgggataatattcagcattggtattgtaagttttggcttgatcatgatatcagcattaattatatatctgcatcctgtacaacaaaatatcactatgaattggtgttctcctttatttaaatatatatatgtaaaaggaaagagaaatttcaaaagaataggaggtgaatcagtcaagtttcttacaaaattgtttcatagctaacatttagataatggagaaataacttaatggaggtataataatttgaaaagtaaattacttaggctattgattgtttccatggtaacgatattggttttttcattagtaggactctctaaggagtcaagtacatctgctaaagaaaaccatacattttctggagaagattactttagaggacttttatttggacaaggggaagttggtaaattaatttcaaacgatttggaccctaaactcgtaaaagaggcaaatagtacagaaggtaaaaagttagtaaatgatgtagtcaaatttataaaaaaagatcaaccacaatatatggatgaattgaaacaatcgattgacagcaaagaccctaaaaaactcattgaaaatatgaccaaagcagaccaacttatccaaaaatatgctaagaaaaatgaaaacgtaaaatactcttctaataaagttactccatcttgtgggctttatgccgtctgtgtagcagctggatatttatatgttgtgggcgttaacgcagttgcattacaaacggctgccgcagtaacaactgcagtgtggaaatacgttgccaaatattcctcttcagcttctaataattctgatttagaagcggctgctgcaaaaaccctaaaattgattcatcaataa BBa_K1074012_sequence 1 ggtaccagctattgtaacataatcggtacgggggtgaaaaagctaacggaaaagggagcggaaaagaatgatgtaagcgtgaaaaattttttatcttatcacttgaaattggaagggagattctttattataagaattgtggaattgtgagcggataacaattcccaatt BBa_K1074015_sequence 1 aaaggaggaaggatca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z