BBa_K1074009 1 BBa_K1074009 SdpA,SdpB,SpbC 2013-09-15T11:00:00Z 2015-05-08T01:09:02Z From the genome of Bacillus subtilis (strain 168) Killing factor of Bacillus subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation. Its maturation is dependent on SdpA and SdpB. Also functions as a ligand, being able to bind to SdpI thereby triggering a signal transduction cascade that protects the cell against the toxic effects of its own SdpC. We use this killing factor to build a kill switch for Bacillus subtilis. false false _1383_ 0 16001 9 Not in stock false With restriction sites of Spe I and Pst I in the sequence. false Changlong Zhao BBa_K1074011 1 BBa_K1074011 PsdpRI+RBS+SDP 2013-09-24T11:00:00Z 2015-05-08T01:09:02Z Promoter PsdpRI and RBS are synthesized via PCR, SDP operon is from genome of Bacillus subtilis We construct this gene circuit as our kill switch to kill the engineered Bacillus subtilis for the safety purpose.Killing is mediated by the exported toxic protein SdpC. Extracellular SdpC induces the synthesis of an immunity protein, SdpI, which protects toxin-producing cells from being killed. SdpI, a polytopic membrane protein, is encoded by a two-gene operon under sporulation control that contains the gene for an autorepressor, SdpR. The autorepressor binds to and blocks the promoter for the operon. Evidence indicates that SdpI is also a signal-transduction protein that responds to the SdpC toxin by sequestering the SdpR autorepressor at the membrane. Sequestration relieves repression and stimulates synthesis of immunity protein. The kill switch is based on a high-copy vector fused with promoter for operon sdpIR and coding sequence for protein SdpC. When SdpC toxins are sensed, they will be captured by Immunity Protein SdpI at the membrane, enabling SdpI to sequester SdpR. As a result, repression on promoter SdpIR is released and more SdpC will be produced. Trapped in this endless loop, the SdpC producing cells fails to cope with enormous toxin SdpC and doomed after eliminating their siblings. Eventually, the group of engineered Bacillus subtilis is destroyed instead of sporulating. false false _1383_ 0 16001 9 Not in stock false no false Changlong Zhao component2362519 1 BBa_K1074016 component2362518 1 BBa_K1074013 component2362520 1 BBa_K1074009 annotation2362520 1 BBa_K1074009 range2362520 1 109 2207 annotation2362518 1 BBa_K1074013 range2362518 1 1 76 annotation2362519 1 BBa_K1074016 range2362519 1 85 102 BBa_K1074016 1 BBa_K1074016 RBS for Pctc 2013-09-24T11:00:00Z 2015-05-08T01:09:02Z From the genome of Bacillus subtilis. synthesized by Sangon Biotech. This is an efficient RBS for promoter Pctc(BBa_K143010) in Bacillus subtilis. It's also an universal RBS for promoters in Bacillus subtilis. We also used it with the promoter PsdpRI(BBa_K1074013) in our project. false false _1383_ 0 16001 9 Not in stock false synthetise with promoter Pgrac as a whole! false Changlong Zhao BBa_K1074013 1 BBa_K1074013 promoter sdpR/I 2013-09-15T11:00:00Z 2015-06-08T02:58:23Z from the genome of Bacillus subtilis WB800N This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis ,and it contains an operon for the signaling pathway of sdpc,a bacterial toxin of B.subtilis. It's activated as the toxin sdpC existed,while the natural pructrue of the promotor, sdpR/I ,confers immunity to the SdpC toxin. Cells of B. subtilis enter the pathway to sporulate under conditions of nutrient limitation could generate sdpC, then this promotor would be activated. We use this part to overexpress sdpC toxin in spore-forming cells,to build a self-killing system in B.subtilis for safety thinking. false false _1383_ 4206 16001 9 In stock false NO false Changlong Zhao BBa_K1074016_sequence 1 aaaggtggtgaatactag BBa_K1074011_sequence 1 gggttgtttttaaaaaaattcaagttataatgaaaataatacatttatacaaatatctaaatgtctaaatgttttttactagagaaaggtggtgaatactagtactagatgactatatgttttctattattttcttcttattactttagcaatatttcacctcagaatccactgttcaaaaaaaattttttgcaacaattgtctccccaaggctttggcttttatagtaaaagccctacagaagaaaacatttcatttcacacaaaagaaaatttaaagttacctaatgcacttcccaataatttttttgggataaaaagagaaggaagagttcaggcaatagaattaggcaaaattgtagagaatatcgatccaaagaattggaaaacttgtgaaaacaacaactcctgcacaaatttagagaaacaaataaagcctattaaggttataaaaaatgaagattatatacatcttagcaaaggagaatacctaatatatcgccaaaaaccactctcatggtattggatagactttaagcaaactacctcttttgaaagaaaggtgctaaaaataaaaatagtatgaagatattaaatagtttagaaggttatattgacacctataatccatggaaaaatacatatgcactttttagaagtttacttggtttctcaacattactagtactattattcaatagtactgatattttatttagttatagtgcaaataatgtcacatgtgaaaatgtctatatccctaccgctttttgttttgctaaagaatatagtatcaattttgagattataagatacttaatgatttttatattaaccttagtggttatagggtggagacctagatttaccggtttatttcactggtatatttgctatagtattcaaacttcagctttaactatcgatggtggagagcaaattgcaactgttctttcttttcttatattacctgttacattattagattcaaggcgaaatcattggaatataaagaaaaacaataatgaatctttcacaaagaagacagtattgttttatataatgacaataattaaaattcaagtttttatcatttatttaaacgcagctttagagcgattgaaaaataaagagtgggcagaaggaacagcaatttactatttcttttctgatccggtgtttggattacctgaatatcaacttaacttaatgaatccactacttgaaagcaattttattgttgtcatcacttggttagtaactatttttgagttgttcttagcagcaagcataatttcaaatatcagaataaagagaattgcccttgttttgggaatattatttcatattgggataatattcagcattggtattgtaagttttggcttgatcatgatatcagcattaattatatatctgcatcctgtacaacaaaatatcactatgaattggtgttctcctttatttaaatatatatatgtaaaaggaaagagaaatttcaaaagaataggaggtgaatcagtcaagtttcttacaaaattgtttcatagctaacatttagataatggagaaataacttaatggaggtataataatttgaaaagtaaattacttaggctattgattgtttccatggtaacgatattggttttttcattagtaggactctctaaggagtcaagtacatctgctaaagaaaaccatacattttctggagaagattactttagaggacttttatttggacaaggggaagttggtaaattaatttcaaacgatttggaccctaaactcgtaaaagaggcaaatagtacagaaggtaaaaagttagtaaatgatgtagtcaaatttataaaaaaagatcaaccacaatatatggatgaattgaaacaatcgattgacagcaaagaccctaaaaaactcattgaaaatatgaccaaagcagaccaacttatccaaaaatatgctaagaaaaatgaaaacgtaaaatactcttctaataaagttactccatcttgtgggctttatgccgtctgtgtagcagctggatatttatatgttgtgggcgttaacgcagttgcattacaaacggctgccgcagtaacaactgcagtgtggaaatacgttgccaaatattcctcttcagcttctaataattctgatttagaagcggctgctgcaaaaaccctaaaattgattcatcaataa BBa_K1074009_sequence 1 atgactatatgttttctattattttcttcttattactttagcaatatttcacctcagaatccactgttcaaaaaaaattttttgcaacaattgtctccccaaggctttggcttttatagtaaaagccctacagaagaaaacatttcatttcacacaaaagaaaatttaaagttacctaatgcacttcccaataatttttttgggataaaaagagaaggaagagttcaggcaatagaattaggcaaaattgtagagaatatcgatccaaagaattggaaaacttgtgaaaacaacaactcctgcacaaatttagagaaacaaataaagcctattaaggttataaaaaatgaagattatatacatcttagcaaaggagaatacctaatatatcgccaaaaaccactctcatggtattggatagactttaagcaaactacctcttttgaaagaaaggtgctaaaaataaaaatagtatgaagatattaaatagtttagaaggttatattgacacctataatccatggaaaaatacatatgcactttttagaagtttacttggtttctcaacattactagtactattattcaatagtactgatattttatttagttatagtgcaaataatgtcacatgtgaaaatgtctatatccctaccgctttttgttttgctaaagaatatagtatcaattttgagattataagatacttaatgatttttatattaaccttagtggttatagggtggagacctagatttaccggtttatttcactggtatatttgctatagtattcaaacttcagctttaactatcgatggtggagagcaaattgcaactgttctttcttttcttatattacctgttacattattagattcaaggcgaaatcattggaatataaagaaaaacaataatgaatctttcacaaagaagacagtattgttttatataatgacaataattaaaattcaagtttttatcatttatttaaacgcagctttagagcgattgaaaaataaagagtgggcagaaggaacagcaatttactatttcttttctgatccggtgtttggattacctgaatatcaacttaacttaatgaatccactacttgaaagcaattttattgttgtcatcacttggttagtaactatttttgagttgttcttagcagcaagcataatttcaaatatcagaataaagagaattgcccttgttttgggaatattatttcatattgggataatattcagcattggtattgtaagttttggcttgatcatgatatcagcattaattatatatctgcatcctgtacaacaaaatatcactatgaattggtgttctcctttatttaaatatatatatgtaaaaggaaagagaaatttcaaaagaataggaggtgaatcagtcaagtttcttacaaaattgtttcatagctaacatttagataatggagaaataacttaatggaggtataataatttgaaaagtaaattacttaggctattgattgtttccatggtaacgatattggttttttcattagtaggactctctaaggagtcaagtacatctgctaaagaaaaccatacattttctggagaagattactttagaggacttttatttggacaaggggaagttggtaaattaatttcaaacgatttggaccctaaactcgtaaaagaggcaaatagtacagaaggtaaaaagttagtaaatgatgtagtcaaatttataaaaaaagatcaaccacaatatatggatgaattgaaacaatcgattgacagcaaagaccctaaaaaactcattgaaaatatgaccaaagcagaccaacttatccaaaaatatgctaagaaaaatgaaaacgtaaaatactcttctaataaagttactccatcttgtgggctttatgccgtctgtgtagcagctggatatttatatgttgtgggcgttaacgcagttgcattacaaacggctgccgcagtaacaactgcagtgtggaaatacgttgccaaatattcctcttcagcttctaataattctgatttagaagcggctgctgcaaaaaccctaaaattgattcatcaataa BBa_K1074013_sequence 1 gggttgtttttaaaaaaattcaagttataatgaaaataatacatttatacaaatatctaaatgtctaaatgttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z