BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_K1075009
1
BBa_K1075009
E. coli sspB[XB]
2013-09-21T11:00:00Z
2015-05-08T01:09:02Z
PCR amplification of full length E. coli sspB. Originally E. Coli sspB is found in the genome of E. coli.
EcsspB itself regulates the degradation of ssrA tagged proteins through the ClpXP protease in procaryotes. In engineered systems it is used to induce degradation of specifically ssrA tagged proteins.
This construct is part of the split system of E. Coli sspB (EcsspB) which was used in fusion with FKBP and FRB to induce the activity of sspB and therefore degradation of proteins with Rapamycins. [1] EcsspB can be functionally divided in three parts: [2] -the N terminal Core domain (113 AA) -the C terminal XB peptide (25 AA) -and a 'flexible linker' in between the first parts (28 AA) We used the same domain structure as is used within the Rapamycin inducable split system. [1] While the Core domain is responsible for dimerization of sspB and binding of ssrA the XB peptide binds the protease ClpXP. The flexible linker is called flexible because it was found, that an increase or reduction in size or amino acid composition does not influence the function of sspB as much as it would in the other domains. [3]
For our project we needed the sspB split system to engineer a light inducable sspB.
false
false
_1384_
0
12108
9
In stock
false
We used the same length of the XB peptide than used for the Rapamycin inducable split system. [1]
false
Max Schelski
BBa_K1075014
1
BBa_K1075014
RBS32-VinD1-sspB[XB]-TT
2013-09-22T11:00:00Z
2015-05-08T01:09:02Z
a
a
false
false
_1384_
0
16105
9
It's complicated
false
a
false
Marc Schulte
component2358790
1
BBa_B0032
component2358800
1
BBa_B0015
component2358793
1
BBa_K1075009
component2358792
1
BBa_K1075011
annotation2358793
1
BBa_K1075009
range2358793
1
802
876
annotation2358790
1
BBa_B0032
range2358790
1
1
13
annotation2358792
1
BBa_K1075011
range2358792
1
20
793
annotation2358800
1
BBa_B0015
range2358800
1
885
1013
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_K1075011
1
BBa_K1075011
Vinculin D1
2013-09-21T11:00:00Z
2015-05-08T01:09:02Z
It was PCR amplified from a plasmid containing full length Vinculin from M. musculus.
This is the D1 Domain of mammalian Vinculin. It forms a tight Dimer with the ipaA peptide.
false
false
_1384_
0
12108
9
It's complicated
false
We used the same amino acid sequence as used for the light inducible heterodimerisation system from Lungu et al. [1]
false
Max Schelski
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1075011_sequence
1
atgccggtgtttcacacgcgtacgatcgagagcatcctggagccggtggcgcagcagatctcgcacctggtgattatgcacgaggagggcgaggtggacggcaaagccattcctgacctcaccgcgcccgtagccgccgtgcaggcggccgtcagcaacctcgtccgggttggaaaagagactgttcagaccactgaggatcagattctgaagagagatatgccaccagcctttattaaggttgaaaatgcttgcaccaagcttgttcaggcagcccagatgcttcagtcagacccatactcggttcctgcgcgggattacctcattgacggctctaggggaatcctttctggcacatctgacctactgcttacctttgatgaggctgaggttcgtaaaattattagggtttgcaaaggaattttggaatatcttacagtggcagaggtagtggaaactatggaagacttggtcacttacacaaagaatcttgggccaggaatgactaagatggccaaaatgattgatgagagacagcaggagttgactcaccaggaacaccgtgtgatgttggtgaactcaatgaacactgtcaaagagctgcttcctgttctcatttcagctatgaagatttttgttacaaccaaaaactcaaaaaaccaaggaatagaagaagctttgaaaaatcgaaattttactgtagaaaagatgagtgctgaaattaacgagatcattcgtgtgttacaactcacttcctgggatgaagacgcctgg
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K1075009_sequence
1
gacactcatcctgacgatgaacctccgcagccaccacgcggtggtcgaccggcattacgcgttgtgaagtaataa
BBa_K1075014_sequence
1
tcacacaggaaagtactagatgccggtgtttcacacgcgtacgatcgagagcatcctggagccggtggcgcagcagatctcgcacctggtgattatgcacgaggagggcgaggtggacggcaaagccattcctgacctcaccgcgcccgtagccgccgtgcaggcggccgtcagcaacctcgtccgggttggaaaagagactgttcagaccactgaggatcagattctgaagagagatatgccaccagcctttattaaggttgaaaatgcttgcaccaagcttgttcaggcagcccagatgcttcagtcagacccatactcggttcctgcgcgggattacctcattgacggctctaggggaatcctttctggcacatctgacctactgcttacctttgatgaggctgaggttcgtaaaattattagggtttgcaaaggaattttggaatatcttacagtggcagaggtagtggaaactatggaagacttggtcacttacacaaagaatcttgggccaggaatgactaagatggccaaaatgattgatgagagacagcaggagttgactcaccaggaacaccgtgtgatgttggtgaactcaatgaacactgtcaaagagctgcttcctgttctcatttcagctatgaagatttttgttacaaccaaaaactcaaaaaaccaaggaatagaagaagctttgaaaaatcgaaattttactgtagaaaagatgagtgctgaaattaacgagatcattcgtgtgttacaactcacttcctgggatgaagacgcctggtactagaggacactcatcctgacgatgaacctccgcagccaccacgcggtggtcgaccggcattacgcgttgtgaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z