LOCUS       BBa_K1075016            2230 bp    DNA     linear       22-Dec-2024
DEFINITION  AraC-pBAD(D)-RBS32-VinD1-sspB[XB]-TT
ACCESSION   BBa_K1075016
VERSION     BBa_K1075016.1  
FEATURES             Location/Qualifiers
     CDS             1..879
                     /label=AraC
     misc_feature    909..924
                     /label=AraO
     misc_feature    1066..1077
                     /label=AraO1
     promoter        1153..1181
                     /label=AraPromoter
     promoter        1..1209
                     /label=BBa_K808000
     RBS             1218..1230
                     /label=RBS-3\Weak
     RBS             1224..1227
                     /label=RBS
     misc_feature    1218..1230
                     /label=BBa_B0032
     RBS             1218..1230
                     /label=BBa_B0032
     misc_feature    1237..2010
                     /label=BBa_K1075011
     misc_feature    2019..2093
                     /label=BBa_K1075009
     stem_loop       2113..2156
                     /label=stem_loop
     misc_feature    2102..2181
                     /label=BBa_B0010
     terminator      2102..2181
                     /label=BBa_B0010
     stem_loop       2197..2216
                     /label=T7 TE
     misc_feature    2223..2223
                     /label=stop
     polyA_site      2217..2230
                     /label=polya
     misc_feature    2190..2230
                     /label=BBa_B0012
     terminator      2190..2230
                     /label=BBa_B0012
     terminator      2102..2230
                     /label=BBa_B0015
     misc_feature    1218..2230
                     /label=BBa_K1075014
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 tgggctaact actagagtca cacaggaaag tactagatgc cggtgtttca cacgcgtacg
     1261 atcgagagca tcctggagcc ggtggcgcag cagatctcgc acctggtgat tatgcacgag
     1321 gagggcgagg tggacggcaa agccattcct gacctcaccg cgcccgtagc cgccgtgcag
     1381 gcggccgtca gcaacctcgt ccgggttgga aaagagactg ttcagaccac tgaggatcag
     1441 attctgaaga gagatatgcc accagccttt attaaggttg aaaatgcttg caccaagctt
     1501 gttcaggcag cccagatgct tcagtcagac ccatactcgg ttcctgcgcg ggattacctc
     1561 attgacggct ctaggggaat cctttctggc acatctgacc tactgcttac ctttgatgag
     1621 gctgaggttc gtaaaattat tagggtttgc aaaggaattt tggaatatct tacagtggca
     1681 gaggtagtgg aaactatgga agacttggtc acttacacaa agaatcttgg gccaggaatg
     1741 actaagatgg ccaaaatgat tgatgagaga cagcaggagt tgactcacca ggaacaccgt
     1801 gtgatgttgg tgaactcaat gaacactgtc aaagagctgc ttcctgttct catttcagct
     1861 atgaagattt ttgttacaac caaaaactca aaaaaccaag gaatagaaga agctttgaaa
     1921 aatcgaaatt ttactgtaga aaagatgagt gctgaaatta acgagatcat tcgtgtgtta
     1981 caactcactt cctgggatga agacgcctgg tactagagga cactcatcct gacgatgaac
     2041 ctccgcagcc accacgcggt ggtcgaccgg cattacgcgt tgtgaagtaa taatactaga
     2101 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     2161 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     2221 tgcgtttata
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