BBa_K1075019
type: ComponentDefinition
persistent identity: BBa K1075019
display id: BBa_K1075019
version: 1
was generated by: iGEM to SBOL conversion
description: AraC-pBAD-RBS32-SspB[Core]-LOV-ipaA-TT
created: 2013-09-22T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _1384_
m user id: 0
owner id: 16105
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Marc Schulte
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K808000
component: BBa_K1075017
sequence annotation: BBa_K808000
sequence annotation: BBa_K1075017
sequence: https://synbiohub.org/public/igem/BBa_K1075019_sequence/1

BBa_K808000
type: Component
persistent identity: component2368747
display id: component2368747
version: 1
top level: BBa_K1075019
definition: Ara
access: public

BBa_K1075017
type: Component
persistent identity: component2368760
display id: component2368760
version: 1
top level: BBa_K1075019
definition: BBa_K1075017
access: public

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2368747
display id: annotation2368747
version: 1
top level: BBa_K1075019
location: https://synbiohub.org/public/igem/BBa_K1075019/annotation2368747/range2368747/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K1075019/annotation2368747/range2368747/1
type: Range
persistent identity: range2368747
display id: range2368747
version: 1
top level: BBa_K1075019
direction: direction/reverse
start: 1
end: 1209
orientation: inline

BBa_K1075017
type: SequenceAnnotation
persistent identity: annotation2368760
display id: annotation2368760
version: 1
top level: BBa_K1075019
location: https://synbiohub.org/public/igem/BBa_K1075019/annotation2368760/range2368760/1
component: BBa_K1075017

https://synbiohub.org/public/igem/BBa_K1075019/annotation2368760/range2368760/1
type: Range
persistent identity: range2368760
display id: range2368760
version: 1
top level: BBa_K1075019
direction: direction/reverse
start: 1218
end: 2213
orientation: inline

Ara
type: ComponentDefinition
persistent identity: BBa K808000
display id: BBa_K808000
version: 1
was generated by: iGEM to SBOL conversion
description: araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
created: 2012-08-10T11:00:00Z
modified: 2015-05-08T01:13:25Z
mutable provenance: Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30 Khlebnikov A, Skaug T, Keasling JD, modulation of gene expression from the arabinose-inducible araBAD promoter,J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This promoter can be induced by arabinose. In presens of arabinose the promotor is active.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: non
star: false
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: AraO
sequence annotation: AraO1
sequence annotation: AraPromoter
sequence: https://synbiohub.org/public/igem/BBa_K808000_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation2200757
display id: annotation2200757
version: 1
top level: Ara
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1
type: Range
persistent identity: range2200757
display id: range2200757
version: 1
top level: Ara
direction: direction/reverse
start: 1
end: 879
orientation: inline

AraO
type: SequenceAnnotation
persistent identity: annotation2200758
display id: annotation2200758
version: 1
top level: Ara
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1
type: Range
persistent identity: range2200758
display id: range2200758
version: 1
top level: Ara
direction: direction/forward
start: 909
end: 924
orientation: inline

AraO1
type: SequenceAnnotation
persistent identity: annotation2200759
display id: annotation2200759
version: 1
top level: Ara
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1
type: Range
persistent identity: range2200759
display id: range2200759
version: 1
top level: Ara
direction: direction/forward
start: 1066
end: 1077
orientation: inline

AraPromoter
type: SequenceAnnotation
persistent identity: annotation2200760
display id: annotation2200760
version: 1
top level: Ara
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1
type: Range
persistent identity: range2200760
display id: range2200760
version: 1
top level: Ara
direction: direction/forward
start: 1153
end: 1181
orientation: inline

BBa_K1075017
type: ComponentDefinition
persistent identity: BBa K1075017
display id: BBa_K1075017
version: 1
was generated by: iGEM to SBOL conversion
description: RBS32-sspB[Core]-LOV-ipaA-TT
created: 2013-09-22T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _1384_
m user id: 0
owner id: 16105
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Marc Schulte
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0032
component: BBa_K1075012
component: BBa_K1075008
component: BBa_B0015
sequence annotation: BBa_B0032
sequence annotation: BBa_K1075008
sequence annotation: BBa_K1075012
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1075017_sequence/1

BBa_B0032
type: Component
persistent identity: component2368732
display id: component2368732
version: 1
top level: BBa_K1075017
definition: BBa_B0032
access: public

BBa_K1075012
type: Component
persistent identity: component2368735
display id: component2368735
version: 1
top level: BBa_K1075017
definition: BBa_K1075012
access: public

BBa_K1075008
type: Component
persistent identity: component2368734
display id: component2368734
version: 1
top level: BBa_K1075017
definition: BBa_K1075008
access: public

BBa_B0015
type: Component
persistent identity: component2368742
display id: component2368742
version: 1
top level: BBa_K1075017
definition: BBa_B0015
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2368732
display id: annotation2368732
version: 1
top level: BBa_K1075017
location: https://synbiohub.org/public/igem/BBa_K1075017/annotation2368732/range2368732/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1075017/annotation2368732/range2368732/1
type: Range
persistent identity: range2368732
display id: range2368732
version: 1
top level: BBa_K1075017
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K1075008
type: SequenceAnnotation
persistent identity: annotation2368734
display id: annotation2368734
version: 1
top level: BBa_K1075017
location: https://synbiohub.org/public/igem/BBa_K1075017/annotation2368734/range2368734/1
component: BBa_K1075008

https://synbiohub.org/public/igem/BBa_K1075017/annotation2368734/range2368734/1
type: Range
persistent identity: range2368734
display id: range2368734
version: 1
top level: BBa_K1075017
direction: direction/reverse
start: 20
end: 358
orientation: inline

BBa_K1075012
type: SequenceAnnotation
persistent identity: annotation2368735
display id: annotation2368735
version: 1
top level: BBa_K1075017
location: https://synbiohub.org/public/igem/BBa_K1075017/annotation2368735/range2368735/1
component: BBa_K1075012

https://synbiohub.org/public/igem/BBa_K1075017/annotation2368735/range2368735/1
type: Range
persistent identity: range2368735
display id: range2368735
version: 1
top level: BBa_K1075017
direction: direction/reverse
start: 365
end: 859
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2368742
display id: annotation2368742
version: 1
top level: BBa_K1075017
location: https://synbiohub.org/public/igem/BBa_K1075017/annotation2368742/range2368742/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1075017/annotation2368742/range2368742/1
type: Range
persistent identity: range2368742
display id: range2368742
version: 1
top level: BBa_K1075017
direction: direction/reverse
start: 868
end: 996
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1075008
type: ComponentDefinition
persistent identity: BBa K1075008
display id: BBa_K1075008
version: 1
was generated by: iGEM to SBOL conversion
description: E. coli sspB[Core]
created: 2013-09-21T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: PCR amplification of full length E. coli sspB. Originally E. Coli sspB is found in the genome of E. coli.
owned by: james
owned by: myers
mutable description: EcsspB itself regulates the degradation of ssrA tagged proteins through the ClpXP protease in procaryotes. In engineered systems it is used to induce degradation of specifically ssrA tagged proteins. This construct is part of the split system of E. Coli sspB (EcsspB) which was used in fusion with FKBP and FRB to induce the activity of sspB and therefore degradation of proteins with Rapamycins. [1] EcsspB can be functionally divided in three parts: [2] -the N terminal Core domain (113 AA) -the C terminal XB peptide (25 AA) -and a 'flexible linker' in between the first parts (28 AA) We used the same domain structure as is used within the Rapamycin inducable split system. [1] While the Core domain is responsible for dimerization of sspB and binding of ssrA the XB peptide binds the protease ClpXP. The flexible linker is called flexible because it was found, that an increase or reduction in size or amino acid composition does not influence the function of sspB as much as it would in the other domains. [3] For our project we needed the sspB split system to engineer a light inducable sspB.
discontinued: false
dominant: false
experience: experience/None
group u list: _1384_
m user id: 0
owner id: 12108
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We used the same length of the XB peptide than used for the Rapamycin inducable split system. [1]
star: false
creator: Max Schelski
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K1075008_sequence/1

BBa_K1075012
type: ComponentDefinition
persistent identity: BBa K1075012
display id: BBa_K1075012
version: 1
was generated by: iGEM to SBOL conversion
description: AsLOV2-ipaA
created: 2013-09-22T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: it was amplified from a plasmid from Addgene - provided by Klaus Hahn. LOV2 originally comes from Avena sativa while the ipaA peptide comes from Shigella spp.
owned by: james
owned by: myers
mutable description: Fusion of LOV 2 (A. sativa) with the ipaA peptide. It forms light inducable a tight dimer with Vinculin. [1]
discontinued: false
dominant: false
group u list: _1384_
m user id: 0
owner id: 12108
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: the fusion protein was designed by Lungu et al. [1]
star: false
creator: Max Schelski
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1075012_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1075019_sequence/1
type: Sequence
persistent identity: BBa K1075019 sequence
display id: BBa_K1075019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgttttttgggctaactactagagtcacacaggaaagtactagatggatttgtcacagctaacaccacgtcgtccctatctgctgcgtgcattctatgagtggttgctggataaccagctcacgccgcacctggtggtggatgtgacgctccctggcgtgcaggttcctatggaatatgcgcgtgacgggcaaatcgtactcaacattgcgccgcgtgctgtcggcaatctggaactggcgaatgatgaggtgcgctttaacgcgcgctttggtggcattccgcgtcaggtttctgtgccgctggctgccgtgctggctatctacgcccgtgaaaatggcgcaggcacgatgttcgaacctgaagctgcctacgatgaagattactagatgcatcatcatcatcatcatggcagcctggcgaccaccctggaacgtattgaaaagaactttgtgattaccgatccgcgtctgccggataacccgattatttttgcgagcgatagctttctgcaactgaccgaatatagccgtgaagaaattctgggccgtaactgccgttttctgcaaggcccggaaaccgatcgtgcgaccgtgcgtaaaattcgtgatgcgattgataaccagaccgaagtgaccgtgcagctgattaactataccaaaagcggcaaaaaattttggaacctgtttcatctgcaaccgatgcgtgatcagaaaggcgatgtgcagtattttattggcgtgcagaaggatggtaccgaacatgtgcgtgatgcggcggaacgtgaaggcgtgatggaaatcaagaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K808000_sequence/1
type: Sequence
persistent identity: BBa K808000 sequence
display id: BBa_K808000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgttttttgggctaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1075017_sequence/1
type: Sequence
persistent identity: BBa K1075017 sequence
display id: BBa_K1075017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggatttgtcacagctaacaccacgtcgtccctatctgctgcgtgcattctatgagtggttgctggataaccagctcacgccgcacctggtggtggatgtgacgctccctggcgtgcaggttcctatggaatatgcgcgtgacgggcaaatcgtactcaacattgcgccgcgtgctgtcggcaatctggaactggcgaatgatgaggtgcgctttaacgcgcgctttggtggcattccgcgtcaggtttctgtgccgctggctgccgtgctggctatctacgcccgtgaaaatggcgcaggcacgatgttcgaacctgaagctgcctacgatgaagattactagatgcatcatcatcatcatcatggcagcctggcgaccaccctggaacgtattgaaaagaactttgtgattaccgatccgcgtctgccggataacccgattatttttgcgagcgatagctttctgcaactgaccgaatatagccgtgaagaaattctgggccgtaactgccgttttctgcaaggcccggaaaccgatcgtgcgaccgtgcgtaaaattcgtgatgcgattgataaccagaccgaagtgaccgtgcagctgattaactataccaaaagcggcaaaaaattttggaacctgtttcatctgcaaccgatgcgtgatcagaaaggcgatgtgcagtattttattggcgtgcagaaggatggtaccgaacatgtgcgtgatgcggcggaacgtgaaggcgtgatggaaatcaagaaaaccgcgaacaacattattaaagcggcgaaagatgtgaccaccagcctgagcaaagtgctgaaaaacattaactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1075008_sequence/1
type: Sequence
persistent identity: BBa K1075008 sequence
display id: BBa_K1075008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggatttgtcacagctaacaccacgtcgtccctatctgctgcgtgcattctatgagtggttgctggataaccagctcacgccgcacctggtggtggatgtgacgctccctggcgtgcaggttcctatggaatatgcgcgtgacgggcaaatcgtactcaacattgcgccgcgtgctgtcggcaatctggaactggcgaatgatgaggtgcgctttaacgcgcgctttggtggcattccgcgtcaggtttctgtgccgctggctgccgtgctggctatctacgcccgtgaaaatggcgcaggcacgatgttcgaacctgaagctgcctacgatgaagat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1075012_sequence/1
type: Sequence
persistent identity: BBa K1075012 sequence
display id: BBa_K1075012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcatcatcatcatcatcatggcagcctggcgaccaccctggaacgtattgaaaagaactttgtgattaccgatccgcgtctgccggataacccgattatttttgcgagcgatagctttctgcaactgaccgaatatagccgtgaagaaattctgggccgtaactgccgttttctgcaaggcccggaaaccgatcgtgcgaccgtgcgtaaaattcgtgatgcgattgataaccagaccgaagtgaccgtgcagctgattaactataccaaaagcggcaaaaaattttggaacctgtttcatctgcaaccgatgcgtgatcagaaaggcgatgtgcagtattttattggcgtgcagaaggatggtaccgaacatgtgcgtgatgcggcggaacgtgaaggcgtgatggaaatcaagaaaaccgcgaacaacattattaaagcggcgaaagatgtgaccaccagcctgagcaaagtgctgaaaaacattaactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z