LOCUS       BBa_K1075024            1657 bp    DNA     linear       26-Dec-2024
DEFINITION  RBS34- mCherry- (Ec)ssrA(DAS+4)-TT
ACCESSION   BBa_K1075024
VERSION     BBa_K1075024.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..729
                     /label=mCherry
     misc_feature    370..370
                     /label=C->T (removing PstI site)
     misc_feature    19..732
                     /label=BBa_J06504
     CDS             739..1446
                     /label=mCherry
     misc_feature    1090..1090
                     /label=C->T (removing PstI site)
     misc_feature    1447..1455
                     /label=LVA
     misc_feature    739..1461
                     /label=BBa_J06505
     misc_feature    1470..1520
                     /label=BBa_K1075020
     stem_loop       1540..1583
                     /label=stem_loop
     misc_feature    1529..1608
                     /label=BBa_B0010
     terminator      1529..1608
                     /label=BBa_B0010
     stem_loop       1624..1643
                     /label=T7 TE
     misc_feature    1650..1650
                     /label=stop
     polyA_site      1644..1657
                     /label=polya
     misc_feature    1617..1657
                     /label=BBa_B0012
     terminator      1617..1657
                     /label=BBa_B0012
     terminator      1529..1657
                     /label=BBa_B0015
     misc_feature    739..1657
                     /label=BBa_K1075023
ORIGIN
        1 aaagaggaga aatactagat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
       61 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      121 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      181 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      241 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      301 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      361 gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      421 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
      481 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
      541 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      601 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
      661 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      721 tacaagtaat aatactagat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
      781 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      841 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      901 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      961 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     1021 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     1081 gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     1141 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1201 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     1261 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     1321 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     1381 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     1441 tacaagttag tagcttaata atactagagg cagctaacga tgaaaactac agcgaaaact
     1501 atgctgacgc tagctaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1561 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1621 actggctcac cttcgggtgg gcctttctgc gtttata
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