##gff-version 3
##sequence-region BBa_K1075025 1 1865
BBa_K1075025	.	engineered_region	209	1865	.	+	0	ID=BBa_K1075024;Name=BBa_K1075024
BBa_K1075025	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1075024
BBa_K1075025	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1075025	.	engineered_region	947	1865	.	+	0	ID=BBa_K1075023;Name=BBa_K1075023;Parent=BBa_K1075024
BBa_K1075025	.	tag	1678	1728	.	+	0	ID=BBa_K1075020;Name=BBa_K1075020;Parent=BBa_K1075023
BBa_K1075025	.	engineered_region	947	1669	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_K1075023
BBa_K1075025	.	tag	1655	1663	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_K1075025	.	sequence_alteration	1298	1298	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_K1075025	.	CDS	947	1654	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_K1075025	.	terminator	1737	1865	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1075023
BBa_K1075025	.	terminator	1737	1816	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1075025	.	stem_loop	1748	1791	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1075025	.	engineered_region	1737	1816	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1075025	.	terminator	1825	1865	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1075025	.	stem_loop	1832	1851	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1075025	.	stop_codon	1858	1858	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1075025	.	engineered_region	1825	1865	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1075025	.	polyA_site	1852	1865	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1075025	.	engineered_region	227	940	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_K1075024
BBa_K1075025	.	sequence_alteration	578	578	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K1075025	.	CDS	227	937	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K1075025	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1075025	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1075025	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1075025	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1075025	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1075025	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1075025	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1075025	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
>BBa_K1075025
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggtgagcaa
gggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccac
gagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtg
gccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccga
catccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtg
gtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccct
ccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccct
gaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggcc
aagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacacca
tcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagat
ggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtg
aacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtga
ccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagca
ccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggac
ggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcacca
acttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgagga
cggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacc
tacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgagg
actacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagt
agcttaataatactagaggcagctaacgatgaaaactacagcgaaaactatgctgacgctagctaataatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata