LOCUS       BBa_K1075026            2874 bp    DNA     linear       22-Nov-2024
DEFINITION  AraC-pBad-RBS34-mCherry-(Ec)ssrA(DAS+4)-TT
ACCESSION   BBa_K1075026
VERSION     BBa_K1075026.1  
FEATURES             Location/Qualifiers
     CDS             1..879
                     /label=AraC
     misc_feature    909..924
                     /label=AraO
     misc_feature    1066..1077
                     /label=AraO1
     promoter        1153..1181
                     /label=AraPromoter
     promoter        1..1209
                     /label=BBa_K808000
     misc_feature    1222..1225
                     /label=conserved
     RBS             1218..1229
                     /label=BBa_B0034
     CDS             1236..1946
                     /label=mCherry
     misc_feature    1587..1587
                     /label=C->T (removing PstI site)
     misc_feature    1236..1949
                     /label=BBa_J06504
     CDS             1956..2663
                     /label=mCherry
     misc_feature    2307..2307
                     /label=C->T (removing PstI site)
     misc_feature    2664..2672
                     /label=LVA
     misc_feature    1956..2678
                     /label=BBa_J06505
     misc_feature    2687..2737
                     /label=BBa_K1075020
     stem_loop       2757..2800
                     /label=stem_loop
     misc_feature    2746..2825
                     /label=BBa_B0010
     terminator      2746..2825
                     /label=BBa_B0010
     stem_loop       2841..2860
                     /label=T7 TE
     misc_feature    2867..2867
                     /label=stop
     polyA_site      2861..2874
                     /label=polya
     misc_feature    2834..2874
                     /label=BBa_B0012
     terminator      2834..2874
                     /label=BBa_B0012
     terminator      2746..2874
                     /label=BBa_B0015
     misc_feature    1956..2874
                     /label=BBa_K1075023
     misc_feature    1218..2874
                     /label=BBa_K1075024
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 tgggctaact actagagaaa gaggagaaat actagatggt gagcaagggc gaggaggata
     1261 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     1321 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     1381 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     1441 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     1501 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     1561 tggtgaccgt gacccaggac tcctccttgc aggacggcga gttcatctac aaggtgaagc
     1621 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     1681 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     1741 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     1801 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     1861 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     1921 gcggcatgga cgagctgtac aagtaataat actagatggt gagcaagggc gaggaggata
     1981 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2041 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2101 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2161 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2221 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2281 tggtgaccgt gacccaggac tcctccttgc aggacggcga gttcatctac aaggtgaagc
     2341 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2401 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     2461 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     2521 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     2581 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     2641 gcggcatgga cgagctgtac aagttagtag cttaataata ctagaggcag ctaacgatga
     2701 aaactacagc gaaaactatg ctgacgctag ctaataatac tagagccagg catcaaataa
     2761 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     2821 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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