LOCUS BBa_K1075027 3467 bp DNA linear 22-Dec-2024 DEFINITION SplitSspB(Rapamycin inducable)-pLac-RBS34-mCherry-(Ec)ssrA(DAS+4)-T T ACCESSION BBa_K1075027 VERSION BBa_K1075027.1 FEATURES Location/Qualifiers misc_feature 1..1594 /label=BBa_K1075010 CDS 1603..1690 /label=end of LacI coding region (inactive) misc_feature 1603..1802 /label=BBa_R0010 misc_feature 1691..1728 /label=CAP binding site promoter 1739..1744 /label=-35 promoter 1763..1768 /label=-10 misc_feature 1768..1802 /label=LacI binding site misc_feature 1775..1775 /label=start promoter 1603..1802 /label=BBa_R0010 misc_feature 1815..1818 /label=conserved RBS 1811..1822 /label=BBa_B0034 CDS 1829..2539 /label=mCherry misc_feature 2180..2180 /label=C->T (removing PstI site) misc_feature 1829..2542 /label=BBa_J06504 CDS 2549..3256 /label=mCherry misc_feature 2900..2900 /label=C->T (removing PstI site) misc_feature 3257..3265 /label=LVA misc_feature 2549..3271 /label=BBa_J06505 misc_feature 3280..3330 /label=BBa_K1075020 stem_loop 3350..3393 /label=stem_loop misc_feature 3339..3418 /label=BBa_B0010 terminator 3339..3418 /label=BBa_B0010 stem_loop 3434..3453 /label=T7 TE misc_feature 3460..3460 /label=stop polyA_site 3454..3467 /label=polya misc_feature 3427..3467 /label=BBa_B0012 terminator 3427..3467 /label=BBa_B0012 terminator 3339..3467 /label=BBa_B0015 misc_feature 2549..3467 /label=BBa_K1075023 misc_feature 1811..3467 /label=BBa_K1075024 misc_feature 1603..3467 /label=BBa_K1075025 ORIGIN 1 gcacagctaa caccacgtcg tccctatctg ctgccctagg tctatgagtg gttgctggat 61 aactttacgg gcatgcataa ggctcgtatg atatattcag ggagaccaca acggtttccc 121 tctacaaata attttgttta acttttacta gagtcacaca ggaaagtact agatgggagt 181 gcaggtggaa accatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg 241 cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa tttgattcct cccgggacag 301 gaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg 361 ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta 421 tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga 481 gcttctaaaa ctggaacacg atgatgacac tcatcctgac gatgaacctc cgcagccacc 541 acgcggtggt cgaccggcat tacgcgttgt gaagtaataa tactagagag agaatataaa 601 aagccagatt attaatccgg cttttttatt attttactag agcacagcta acaccacgtc 661 gtccctatct gctgccctag gtctatgagt ggttgctgga taactttacg ggcatgcata 721 aggctcgtaa tatatattca gggagaccac aacggtttcc ctctacaaat aattttgttt 781 aacttttact agagtcacac aggaaagtac tagatggatt tgtcacagct aacaccacgt 841 cgtccctatc tgctgcgtgc attctatgag tggttgctgg ataaccagct cacgccgcac 901 ctggtggtgg atgtgacgct ccctggcgtg caggttccta tggaatatgc gcgtgacggg 961 caaatcgtac tcaacattgc gccgcgtgct gtcggcaatc tggaactggc gaatgatgag 1021 gtgcgcttta acgcgcgctt tggtggcatt ccgcgtcagg tttctgtgcc gctggctgcc 1081 gtgctggcta tctacgcccg tgaaaatggc gcaggcacga tgttcgaacc tgaagctgcc 1141 tacgatgaag atcaccacca ccaccaccac cgcggatccg agctgattcg agtagccatc 1201 ctctggcatg agatgtggca tgaaggccta gaagaggcct ctcgcttgta ctttggggag 1261 aggaacgtca aaggcatgtt tgaggtgctg gagcccctgc atgctatgat ggaacgcggt 1321 ccccagaccc tgaaggaaac gtcctttaat caggcgtatg gtcgagattt aatggaggca 1381 caagaatggt gccgaaagta catgaaatca gggaacgtca aggacctcac ccaagcctgg 1441 gacctctact atcacgtgtt cagacggatc tccaagcagt aataatacta gagagagaat 1501 ataaaaagcc agattattaa tccggctttt ttattatttt actagagaga gaatataaaa 1561 agccagatta ttaatccggc ttttttatta tttttactag agcaatacgc aaaccgcctc 1621 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 1681 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 1741 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 1801 catactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg ataacatggc 1861 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga 1921 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct 1981 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat 2041 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc 2101 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac 2161 cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg 2221 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc 2281 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa 2341 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc 2401 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga 2461 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat 2521 ggacgagctg tacaagtaat aatactagat ggtgagcaag ggcgaggagg ataacatggc 2581 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga 2641 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct 2701 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat 2761 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc 2821 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac 2881 cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg 2941 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc 3001 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa 3061 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc 3121 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga 3181 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat 3241 ggacgagctg tacaagttag tagcttaata atactagagg cagctaacga tgaaaactac 3301 agcgaaaact atgctgacgc tagctaataa tactagagcc aggcatcaaa taaaacgaaa 3361 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 3421 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //