##gff-version 3
##sequence-region BBa_K1075027 1 3467
BBa_K1075027	.	engineered_region	1	1594	.	+	0	ID=BBa_K1075010;Name=BBa_K1075010
BBa_K1075027	.	engineered_region	1603	3467	.	+	0	ID=BBa_K1075025;Name=BBa_K1075025
BBa_K1075027	.	engineered_region	1811	3467	.	+	0	ID=BBa_K1075024;Name=BBa_K1075024;Parent=BBa_K1075025
BBa_K1075027	.	engineered_region	2549	3467	.	+	0	ID=BBa_K1075023;Name=BBa_K1075023;Parent=BBa_K1075024
BBa_K1075027	.	terminator	3339	3467	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1075023
BBa_K1075027	.	terminator	3339	3418	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1075027	.	stem_loop	3350	3393	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1075027	.	engineered_region	3339	3418	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1075027	.	terminator	3427	3467	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1075027	.	engineered_region	3427	3467	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1075027	.	stop_codon	3460	3460	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1075027	.	stem_loop	3434	3453	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1075027	.	polyA_site	3454	3467	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1075027	.	engineered_region	2549	3271	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_K1075023
BBa_K1075027	.	CDS	2549	3256	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_K1075027	.	tag	3257	3265	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_K1075027	.	sequence_alteration	2900	2900	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_K1075027	.	tag	3280	3330	.	+	0	ID=BBa_K1075020;Name=BBa_K1075020;Parent=BBa_K1075023
BBa_K1075027	.	engineered_region	1829	2542	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_K1075024
BBa_K1075027	.	sequence_alteration	2180	2180	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K1075027	.	CDS	1829	2539	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K1075027	.	ribosome_entry_site	1811	1822	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1075024
BBa_K1075027	.	conserved_region	1815	1818	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1075027	.	promoter	1603	1802	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1075025
BBa_K1075027	.	non_covalent_binding_site	1768	1802	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1075027	.	non_covalent_binding_site	1691	1728	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1075027	.	engineered_region	1603	1802	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1075027	.	promoter	1739	1744	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1075027	.	CDS	1603	1690	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1075027	.	start_codon	1775	1775	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1075027	.	promoter	1763	1768	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
>BBa_K1075027
gcacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgggcatgcata
aggctcgtatgatatattcagggagaccacaacggtttccctctacaaataattttgtttaacttttactagagtcaca
caggaaagtactagatgggagtgcaggtggaaaccatctccccaggagacgggcgcaccttccccaagcgcggccagac
ctgcgtggtgcactacaccgggatgcttgaagatggaaagaaatttgattcctcccgggacaggaacaagccctttaag
tttatgctaggcaagcaggaggtgatccgaggctgggaagaaggggttgcccagatgagtgtgggtcagagagccaaac
tgactatatctccagattatgcctatggtgccactgggcacccaggcatcatcccaccacatgccactctcgtcttcga
tgtggagcttctaaaactggaacacgatgatgacactcatcctgacgatgaacctccgcagccaccacgcggtggtcga
ccggcattacgcgttgtgaagtaataatactagagagagaatataaaaagccagattattaatccggcttttttattat
tttactagagcacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgg
gcatgcataaggctcgtaatatatattcagggagaccacaacggtttccctctacaaataattttgtttaacttttact
agagtcacacaggaaagtactagatggatttgtcacagctaacaccacgtcgtccctatctgctgcgtgcattctatga
gtggttgctggataaccagctcacgccgcacctggtggtggatgtgacgctccctggcgtgcaggttcctatggaatat
gcgcgtgacgggcaaatcgtactcaacattgcgccgcgtgctgtcggcaatctggaactggcgaatgatgaggtgcgct
ttaacgcgcgctttggtggcattccgcgtcaggtttctgtgccgctggctgccgtgctggctatctacgcccgtgaaaa
tggcgcaggcacgatgttcgaacctgaagctgcctacgatgaagatcaccaccaccaccaccaccgcggatccgagctg
attcgagtagccatcctctggcatgagatgtggcatgaaggcctagaagaggcctctcgcttgtactttggggagagga
acgtcaaaggcatgtttgaggtgctggagcccctgcatgctatgatggaacgcggtccccagaccctgaaggaaacgtc
ctttaatcaggcgtatggtcgagatttaatggaggcacaagaatggtgccgaaagtacatgaaatcagggaacgtcaag
gacctcacccaagcctgggacctctactatcacgtgttcagacggatctccaagcagtaataatactagagagagaata
taaaaagccagattattaatccggcttttttattattttactagagagagaatataaaaagccagattattaatccggc
ttttttattatttttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacg
acaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggc
tttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagagg
agaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacat
ggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgcc
aagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaagg
cctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgat
gaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaag
ctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcgga
tgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctga
ggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacc
tcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagc
tgtacaagtaataatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaa
ggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacc
cagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacg
gctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtggga
gcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctac
aaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcct
ccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccacta
cgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttg
gacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggca
tggacgagctgtacaagttagtagcttaataatactagaggcagctaacgatgaaaactacagcgaaaactatgctgac
gctagctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata