BBa_K1075027
type: ComponentDefinition
persistent identity: BBa K1075027
display id: BBa_K1075027
version: 1
was generated by: iGEM to SBOL conversion
description: SplitSspB(Rapamycin inducable)-pLac-RBS34-mCherry-(Ec)ssrA(DAS+4)-TT
created: 2013-09-22T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _1384_
m user id: 0
owner id: 16105
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Marc Schulte
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1075010
component: BBa_K1075025
sequence annotation: BBa_K1075010
sequence annotation: BBa_K1075025
sequence: https://synbiohub.org/public/igem/BBa_K1075027_sequence/1

BBa_K1075010
type: Component
persistent identity: component2374243
display id: component2374243
version: 1
top level: BBa_K1075027
definition: BBa_K1075010
access: public

BBa_K1075025
type: Component
persistent identity: component2374270
display id: component2374270
version: 1
top level: BBa_K1075027
definition: BBa_K1075025
access: public

BBa_K1075010
type: SequenceAnnotation
persistent identity: annotation2374243
display id: annotation2374243
version: 1
top level: BBa_K1075027
location: https://synbiohub.org/public/igem/BBa_K1075027/annotation2374243/range2374243/1
component: BBa_K1075010

https://synbiohub.org/public/igem/BBa_K1075027/annotation2374243/range2374243/1
type: Range
persistent identity: range2374243
display id: range2374243
version: 1
top level: BBa_K1075027
direction: direction/reverse
start: 1
end: 1594
orientation: inline

BBa_K1075025
type: SequenceAnnotation
persistent identity: annotation2374270
display id: annotation2374270
version: 1
top level: BBa_K1075027
location: https://synbiohub.org/public/igem/BBa_K1075027/annotation2374270/range2374270/1
component: BBa_K1075025

https://synbiohub.org/public/igem/BBa_K1075027/annotation2374270/range2374270/1
type: Range
persistent identity: range2374270
display id: range2374270
version: 1
top level: BBa_K1075027
direction: direction/reverse
start: 1603
end: 3467
orientation: inline

BBa_K1075010
type: ComponentDefinition
persistent identity: BBa K1075010
display id: BBa_K1075010
version: 1
was generated by: iGEM to SBOL conversion
description: pC-RBS32-FKBP12-EcsspB[XB]-Term-pB-RBS32-EcsspB[Core]-FRB-Term-Term (Rapamycin inducable EcsspB)
created: 2013-09-21T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: it was assembled by Joey Davis
owned by: james
owned by: myers
mutable description: This part is used to induce the function of sspB with rapamycin. It was originally characterized in pSB3C5 The part was designed by Joey Davis. [1]
discontinued: false
dominant: false
experience: experience/None
group u list: _1384_
m user id: 0
owner id: 12108
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Joey Davis designed the part
star: false
creator: Max Schelski
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1075010_sequence/1

BBa_K1075025
type: ComponentDefinition
persistent identity: BBa K1075025
display id: BBa_K1075025
version: 1
was generated by: iGEM to SBOL conversion
description: pLac-RBS34-mCherry-(Ec)ssrA(DAS+4)-TT
created: 2013-09-22T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/Works
group u list: _1384_
m user id: 0
owner id: 16105
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Marc Schulte
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_R0010
component: BBa_K1075024
sequence annotation: BBa_R0010
sequence annotation: BBa_K1075024
sequence: https://synbiohub.org/public/igem/BBa_K1075025_sequence/1

BBa_R0010
type: Component
persistent identity: component2373803
display id: component2373803
version: 1
top level: BBa_K1075025
definition: LacI
access: public

BBa_K1075024
type: Component
persistent identity: component2373828
display id: component2373828
version: 1
top level: BBa_K1075025
definition: BBa_K1075024
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2373803
display id: annotation2373803
version: 1
top level: BBa_K1075025
location: https://synbiohub.org/public/igem/BBa_K1075025/annotation2373803/range2373803/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1075025/annotation2373803/range2373803/1
type: Range
persistent identity: range2373803
display id: range2373803
version: 1
top level: BBa_K1075025
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K1075024
type: SequenceAnnotation
persistent identity: annotation2373828
display id: annotation2373828
version: 1
top level: BBa_K1075025
location: https://synbiohub.org/public/igem/BBa_K1075025/annotation2373828/range2373828/1
component: BBa_K1075024

https://synbiohub.org/public/igem/BBa_K1075025/annotation2373828/range2373828/1
type: Range
persistent identity: range2373828
display id: range2373828
version: 1
top level: BBa_K1075025
direction: direction/reverse
start: 209
end: 1865
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_K1075024
type: ComponentDefinition
persistent identity: BBa K1075024
display id: BBa_K1075024
version: 1
was generated by: iGEM to SBOL conversion
description: RBS34- mCherry- (Ec)ssrA(DAS+4)-TT
created: 2013-09-22T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _1384_
m user id: 0
owner id: 16105
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Marc Schulte
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J06504
component: BBa_B0034
component: BBa_K1075023
sequence annotation: BBa_B0034
sequence annotation: BBa_J06504
sequence annotation: BBa_K1075023
sequence: https://synbiohub.org/public/igem/BBa_K1075024_sequence/1

BBa_J06504
type: Component
persistent identity: component2373597
display id: component2373597
version: 1
top level: BBa_K1075024
definition: mCherry
access: public

BBa_B0034
type: Component
persistent identity: component2373594
display id: component2373594
version: 1
top level: BBa_K1075024
definition: BBa_B0034
access: public

BBa_K1075023
type: Component
persistent identity: component2373610
display id: component2373610
version: 1
top level: BBa_K1075024
definition: BBa_K1075023
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2373594
display id: annotation2373594
version: 1
top level: BBa_K1075024
location: https://synbiohub.org/public/igem/BBa_K1075024/annotation2373594/range2373594/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1075024/annotation2373594/range2373594/1
type: Range
persistent identity: range2373594
display id: range2373594
version: 1
top level: BBa_K1075024
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation2373597
display id: annotation2373597
version: 1
top level: BBa_K1075024
location: https://synbiohub.org/public/igem/BBa_K1075024/annotation2373597/range2373597/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_K1075024/annotation2373597/range2373597/1
type: Range
persistent identity: range2373597
display id: range2373597
version: 1
top level: BBa_K1075024
direction: direction/reverse
start: 19
end: 732
orientation: inline

BBa_K1075023
type: SequenceAnnotation
persistent identity: annotation2373610
display id: annotation2373610
version: 1
top level: BBa_K1075024
location: https://synbiohub.org/public/igem/BBa_K1075024/annotation2373610/range2373610/1
component: BBa_K1075023

https://synbiohub.org/public/igem/BBa_K1075024/annotation2373610/range2373610/1
type: Range
persistent identity: range2373610
display id: range2373610
version: 1
top level: BBa_K1075024
direction: direction/reverse
start: 739
end: 1657
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06504
display id: BBa_J06504
version: 1
was generated by: iGEM to SBOL conversion
description: monomeric RFP optimized for bacteria
created: 2005-07-17T11:00:00Z
modified: 2016-01-25T01:05:28Z
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 4206
owner id: 340
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
star: true
creator: ytwang
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence: https://synbiohub.org/public/igem/BBa_J06504_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585829
display id: annotation1585829
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
type: Range
persistent identity: range1585829
display id: range1585829
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 711
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585833
display id: annotation1585833
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
type: Range
persistent identity: range1585833
display id: range1585833
version: 1
top level: mCherry
direction: direction/forward
start: 352
end: 352
orientation: inline

BBa_K1075023
type: ComponentDefinition
persistent identity: BBa K1075023
display id: BBa_K1075023
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry- (Ec)ssrA(DAS+4)-TT
created: 2013-09-22T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _1384_
m user id: 0
owner id: 16105
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Marc Schulte
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J06505
component: BBa_B0015
component: BBa_K1075020
sequence annotation: BBa_J06505
sequence annotation: BBa_K1075020
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1075023_sequence/1

BBa_J06505
type: Component
persistent identity: component2369478
display id: component2369478
version: 1
top level: BBa_K1075023
definition: mCherry
access: public

BBa_B0015
type: Component
persistent identity: component2369486
display id: component2369486
version: 1
top level: BBa_K1075023
definition: BBa_B0015
access: public

BBa_K1075020
type: Component
persistent identity: component2369479
display id: component2369479
version: 1
top level: BBa_K1075023
definition: ssrA(DAS+4
access: public

BBa_J06505
type: SequenceAnnotation
persistent identity: annotation2369478
display id: annotation2369478
version: 1
top level: BBa_K1075023
location: https://synbiohub.org/public/igem/BBa_K1075023/annotation2369478/range2369478/1
component: BBa_J06505

https://synbiohub.org/public/igem/BBa_K1075023/annotation2369478/range2369478/1
type: Range
persistent identity: range2369478
display id: range2369478
version: 1
top level: BBa_K1075023
direction: direction/reverse
start: 1
end: 723
orientation: inline

BBa_K1075020
type: SequenceAnnotation
persistent identity: annotation2369479
display id: annotation2369479
version: 1
top level: BBa_K1075023
location: https://synbiohub.org/public/igem/BBa_K1075023/annotation2369479/range2369479/1
component: BBa_K1075020

https://synbiohub.org/public/igem/BBa_K1075023/annotation2369479/range2369479/1
type: Range
persistent identity: range2369479
display id: range2369479
version: 1
top level: BBa_K1075023
direction: direction/reverse
start: 732
end: 782
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2369486
display id: annotation2369486
version: 1
top level: BBa_K1075023
location: https://synbiohub.org/public/igem/BBa_K1075023/annotation2369486/range2369486/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1075023/annotation2369486/range2369486/1
type: Range
persistent identity: range2369486
display id: range2369486
version: 1
top level: BBa_K1075023
direction: direction/reverse
start: 791
end: 919
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

ssrA(DAS+4
type: ComponentDefinition
persistent identity: BBa K1075020
display id: BBa_K1075020
version: 1
was generated by: iGEM to SBOL conversion
description: E. coli ssrA(DAS+4)
created: 2013-09-22T11:00:00Z
modified: 2015-05-08T01:09:02Z
mutable provenance: PCR amplification from gene synthesis
owned by: james
owned by: myers
mutable description: fused to the C terminus of a protein it triggers degradation upon induction of sspB function.
discontinued: false
dominant: false
experience: experience/None
group u list: _1384_
m user id: 0
owner id: 12108
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: we used the E. coli ssrA tag with a weakened interaction with ClpXP and a linker of 4 amino acids in between to optimize degradation speed increase upon induction with sspB. [1]
star: false
creator: Max Schelski
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K1075020_sequence/1

mCherry
type: ComponentDefinition
persistent identity: BBa J06505
display id: BBa_J06505
version: 1
was generated by: iGEM to SBOL conversion
description: monomeric RFP optimized for bacteria
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:08:18Z
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible and with an LVA tag added.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends with the LVA tag using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
star: false
creator: ytwang
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_J06505_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585879
display id: annotation1585879
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585879/range1585879/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J06505/annotation1585879/range1585879/1
type: Range
persistent identity: range1585879
display id: range1585879
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 708
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585880
display id: annotation1585880
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585880/range1585880/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J06505/annotation1585880/range1585880/1
type: Range
persistent identity: range1585880
display id: range1585880
version: 1
top level: mCherry
direction: direction/forward
start: 352
end: 352
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1585887
display id: annotation1585887
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585887/range1585887/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J06505/annotation1585887/range1585887/1
type: Range
persistent identity: range1585887
display id: range1585887
version: 1
top level: mCherry
direction: direction/forward
start: 709
end: 717
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1075027_sequence/1
type: Sequence
persistent identity: BBa K1075027 sequence
display id: BBa_K1075027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgggcatgcataaggctcgtatgatatattcagggagaccacaacggtttccctctacaaataattttgtttaacttttactagagtcacacaggaaagtactagatgggagtgcaggtggaaaccatctccccaggagacgggcgcaccttccccaagcgcggccagacctgcgtggtgcactacaccgggatgcttgaagatggaaagaaatttgattcctcccgggacaggaacaagccctttaagtttatgctaggcaagcaggaggtgatccgaggctgggaagaaggggttgcccagatgagtgtgggtcagagagccaaactgactatatctccagattatgcctatggtgccactgggcacccaggcatcatcccaccacatgccactctcgtcttcgatgtggagcttctaaaactggaacacgatgatgacactcatcctgacgatgaacctccgcagccaccacgcggtggtcgaccggcattacgcgttgtgaagtaataatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagcacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgggcatgcataaggctcgtaatatatattcagggagaccacaacggtttccctctacaaataattttgtttaacttttactagagtcacacaggaaagtactagatggatttgtcacagctaacaccacgtcgtccctatctgctgcgtgcattctatgagtggttgctggataaccagctcacgccgcacctggtggtggatgtgacgctccctggcgtgcaggttcctatggaatatgcgcgtgacgggcaaatcgtactcaacattgcgccgcgtgctgtcggcaatctggaactggcgaatgatgaggtgcgctttaacgcgcgctttggtggcattccgcgtcaggtttctgtgccgctggctgccgtgctggctatctacgcccgtgaaaatggcgcaggcacgatgttcgaacctgaagctgcctacgatgaagatcaccaccaccaccaccaccgcggatccgagctgattcgagtagccatcctctggcatgagatgtggcatgaaggcctagaagaggcctctcgcttgtactttggggagaggaacgtcaaaggcatgtttgaggtgctggagcccctgcatgctatgatggaacgcggtccccagaccctgaaggaaacgtcctttaatcaggcgtatggtcgagatttaatggaggcacaagaatggtgccgaaagtacatgaaatcagggaacgtcaaggacctcacccaagcctgggacctctactatcacgtgttcagacggatctccaagcagtaataatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagagagaatataaaaagccagattattaatccggcttttttattatttttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggcc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1075010_sequence/1
type: Sequence
persistent identity: BBa K1075010 sequence
display id: BBa_K1075010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgggcatgcataaggctcgtatgatatattcagggagaccacaacggtttccctctacaaataattttgtttaacttttactagagtcacacaggaaagtactagatgggagtgcaggtggaaaccatctccccaggagacgggcgcaccttccccaagcgcggccagacctgcgtggtgcactacaccgggatgcttgaagatggaaagaaatttgattcctcccgggacaggaacaagccctttaagtttatgctaggcaagcaggaggtgatccgaggctgggaagaaggggttgcccagatgagtgtgggtcagagagccaaactgactatatctccagattatgcctatggtgccactgggcacccaggcatcatcccaccacatgccactctcgtcttcgatgtggagcttctaaaactggaacacgatgatgacactcatcctgacgatgaacctccgcagccaccacgcggtggtcgaccggcattacgcgttgtgaagtaataatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagcacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgggcatgcataaggctcgtaatatatattcagggagaccacaacggtttccctctacaaataattttgtttaacttttactagagtcacacaggaaagtactagatggatttgtcacagctaacaccacgtcgtccctatctgctgcgtgcattctatgagtggttgctggataaccagctcacgccgcacctggtggtggatgtgacgctccctggcgtgcaggttcctatggaatatgcgcgtgacgggcaaatcgtactcaacattgcgccgcgtgctgtcggcaatctggaactggcgaatgatgaggtgcgctttaacgcgcgctttggtggcattccgcgtcaggtttctgtgccgctggctgccgtgctggctatctacgcccgtgaaaatggcgcaggcacgatgttcgaacctgaagctgcctacgatgaagatcaccaccaccaccaccaccgcggatccgagctgattcgagtagccatcctctggcatgagatgtggcatgaaggcctagaagaggcctctcgcttgtactttggggagaggaacgtcaaaggcatgtttgaggtgctggagcccctgcatgctatgatggaacgcggtccccagaccctgaaggaaacgtcctttaatcaggcgtatggtcgagatttaatggaggcacaagaatggtgccgaaagtacatgaaatcagggaacgtcaaggacctcacccaagcctgggacctctactatcacgtgttcagacggatctccaagcagtaataatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagagagaatataaaaagccagattattaatccggcttttttattatttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1075025_sequence/1
type: Sequence
persistent identity: BBa K1075025 sequence
display id: BBa_K1075025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataatactagaggcagctaacgatgaaaactacagcgaaaactatgctgacgctagctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1075024_sequence/1
type: Sequence
persistent identity: BBa K1075024 sequence
display id: BBa_K1075024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataatactagaggcagctaacgatgaaaactacagcgaaaactatgctgacgctagctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06504_sequence/1
type: Sequence
persistent identity: BBa J06504 sequence
display id: BBa_J06504_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1075023_sequence/1
type: Sequence
persistent identity: BBa K1075023 sequence
display id: BBa_K1075023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataatactagaggcagctaacgatgaaaactacagcgaaaactatgctgacgctagctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1075020_sequence/1
type: Sequence
persistent identity: BBa K1075020 sequence
display id: BBa_K1075020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcagctaacgatgaaaactacagcgaaaactatgctgacgctagctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06505_sequence/1
type: Sequence
persistent identity: BBa J06505 sequence
display id: BBa_J06505_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z