LOCUS       BBa_K1075028            2679 bp    DNA     linear       23-Dec-2024
DEFINITION  SplitSspB(Rapamycin inducable)-pLac-RBS34-mCherry-TT
ACCESSION   BBa_K1075028
VERSION     BBa_K1075028.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1594
                     /label=BBa_K1075010
     CDS             1603..1690
                     /label=end of LacI coding region (inactive)
     misc_feature    1603..1802
                     /label=BBa_R0010
     misc_feature    1691..1728
                     /label=CAP binding site
     promoter        1739..1744
                     /label=-35
     promoter        1763..1768
                     /label=-10
     misc_feature    1768..1802
                     /label=LacI binding site
     misc_feature    1775..1775
                     /label=start
     promoter        1603..1802
                     /label=BBa_R0010
     misc_feature    1815..1818
                     /label=conserved
     RBS             1811..1822
                     /label=BBa_B0034
     CDS             1829..2539
                     /label=mCherry
     misc_feature    2180..2180
                     /label=C->T (removing PstI site)
     misc_feature    1829..2542
                     /label=BBa_J06504
     stem_loop       2562..2605
                     /label=stem_loop
     misc_feature    2551..2630
                     /label=BBa_B0010
     terminator      2551..2630
                     /label=BBa_B0010
     stem_loop       2646..2665
                     /label=T7 TE
     misc_feature    2672..2672
                     /label=stop
     polyA_site      2666..2679
                     /label=polya
     misc_feature    2639..2679
                     /label=BBa_B0012
     terminator      2639..2679
                     /label=BBa_B0012
     misc_feature    1603..2679
                     /label=BBa_K204044
ORIGIN
        1 gcacagctaa caccacgtcg tccctatctg ctgccctagg tctatgagtg gttgctggat
       61 aactttacgg gcatgcataa ggctcgtatg atatattcag ggagaccaca acggtttccc
      121 tctacaaata attttgttta acttttacta gagtcacaca ggaaagtact agatgggagt
      181 gcaggtggaa accatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg
      241 cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa tttgattcct cccgggacag
      301 gaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg
      361 ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta
      421 tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga
      481 gcttctaaaa ctggaacacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
      541 acgcggtggt cgaccggcat tacgcgttgt gaagtaataa tactagagag agaatataaa
      601 aagccagatt attaatccgg cttttttatt attttactag agcacagcta acaccacgtc
      661 gtccctatct gctgccctag gtctatgagt ggttgctgga taactttacg ggcatgcata
      721 aggctcgtaa tatatattca gggagaccac aacggtttcc ctctacaaat aattttgttt
      781 aacttttact agagtcacac aggaaagtac tagatggatt tgtcacagct aacaccacgt
      841 cgtccctatc tgctgcgtgc attctatgag tggttgctgg ataaccagct cacgccgcac
      901 ctggtggtgg atgtgacgct ccctggcgtg caggttccta tggaatatgc gcgtgacggg
      961 caaatcgtac tcaacattgc gccgcgtgct gtcggcaatc tggaactggc gaatgatgag
     1021 gtgcgcttta acgcgcgctt tggtggcatt ccgcgtcagg tttctgtgcc gctggctgcc
     1081 gtgctggcta tctacgcccg tgaaaatggc gcaggcacga tgttcgaacc tgaagctgcc
     1141 tacgatgaag atcaccacca ccaccaccac cgcggatccg agctgattcg agtagccatc
     1201 ctctggcatg agatgtggca tgaaggccta gaagaggcct ctcgcttgta ctttggggag
     1261 aggaacgtca aaggcatgtt tgaggtgctg gagcccctgc atgctatgat ggaacgcggt
     1321 ccccagaccc tgaaggaaac gtcctttaat caggcgtatg gtcgagattt aatggaggca
     1381 caagaatggt gccgaaagta catgaaatca gggaacgtca aggacctcac ccaagcctgg
     1441 gacctctact atcacgtgtt cagacggatc tccaagcagt aataatacta gagagagaat
     1501 ataaaaagcc agattattaa tccggctttt ttattatttt actagagaga gaatataaaa
     1561 agccagatta ttaatccggc ttttttatta tttttactag agcaatacgc aaaccgcctc
     1621 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1681 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     1741 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     1801 catactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg ataacatggc
     1861 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     1921 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     1981 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     2041 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     2101 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     2161 cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     2221 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     2281 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     2341 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     2401 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     2461 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     2521 ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga aaggctcagt
     2581 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2641 acactggctc accttcgggt gggcctttct gcgtttata
//