##gff-version 3
##sequence-region BBa_K1075028 1 2679
BBa_K1075028	.	engineered_region	1	1594	.	+	0	ID=BBa_K1075010;Name=BBa_K1075010
BBa_K1075028	.	engineered_region	1603	2679	.	+	0	ID=BBa_K204044;Name=BBa_K204044
BBa_K1075028	.	engineered_region	1829	2542	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_K204044
BBa_K1075028	.	CDS	1829	2539	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K1075028	.	sequence_alteration	2180	2180	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K1075028	.	terminator	2551	2630	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K204044
BBa_K1075028	.	stem_loop	2562	2605	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1075028	.	engineered_region	2551	2630	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1075028	.	promoter	1603	1802	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K204044
BBa_K1075028	.	non_covalent_binding_site	1691	1728	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1075028	.	promoter	1739	1744	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1075028	.	promoter	1763	1768	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1075028	.	start_codon	1775	1775	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1075028	.	engineered_region	1603	1802	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1075028	.	CDS	1603	1690	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1075028	.	non_covalent_binding_site	1768	1802	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1075028	.	terminator	2639	2679	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K204044
BBa_K1075028	.	polyA_site	2666	2679	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1075028	.	stem_loop	2646	2665	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1075028	.	stop_codon	2672	2672	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1075028	.	engineered_region	2639	2679	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1075028	.	ribosome_entry_site	1811	1822	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K204044
BBa_K1075028	.	conserved_region	1815	1818	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1075028
gcacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgggcatgcata
aggctcgtatgatatattcagggagaccacaacggtttccctctacaaataattttgtttaacttttactagagtcaca
caggaaagtactagatgggagtgcaggtggaaaccatctccccaggagacgggcgcaccttccccaagcgcggccagac
ctgcgtggtgcactacaccgggatgcttgaagatggaaagaaatttgattcctcccgggacaggaacaagccctttaag
tttatgctaggcaagcaggaggtgatccgaggctgggaagaaggggttgcccagatgagtgtgggtcagagagccaaac
tgactatatctccagattatgcctatggtgccactgggcacccaggcatcatcccaccacatgccactctcgtcttcga
tgtggagcttctaaaactggaacacgatgatgacactcatcctgacgatgaacctccgcagccaccacgcggtggtcga
ccggcattacgcgttgtgaagtaataatactagagagagaatataaaaagccagattattaatccggcttttttattat
tttactagagcacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgg
gcatgcataaggctcgtaatatatattcagggagaccacaacggtttccctctacaaataattttgtttaacttttact
agagtcacacaggaaagtactagatggatttgtcacagctaacaccacgtcgtccctatctgctgcgtgcattctatga
gtggttgctggataaccagctcacgccgcacctggtggtggatgtgacgctccctggcgtgcaggttcctatggaatat
gcgcgtgacgggcaaatcgtactcaacattgcgccgcgtgctgtcggcaatctggaactggcgaatgatgaggtgcgct
ttaacgcgcgctttggtggcattccgcgtcaggtttctgtgccgctggctgccgtgctggctatctacgcccgtgaaaa
tggcgcaggcacgatgttcgaacctgaagctgcctacgatgaagatcaccaccaccaccaccaccgcggatccgagctg
attcgagtagccatcctctggcatgagatgtggcatgaaggcctagaagaggcctctcgcttgtactttggggagagga
acgtcaaaggcatgtttgaggtgctggagcccctgcatgctatgatggaacgcggtccccagaccctgaaggaaacgtc
ctttaatcaggcgtatggtcgagatttaatggaggcacaagaatggtgccgaaagtacatgaaatcagggaacgtcaag
gacctcacccaagcctgggacctctactatcacgtgttcagacggatctccaagcagtaataatactagagagagaata
taaaaagccagattattaatccggcttttttattattttactagagagagaatataaaaagccagattattaatccggc
ttttttattatttttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacg
acaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggc
tttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagagg
agaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacat
ggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgcc
aagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaagg
cctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgat
gaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaag
ctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcgga
tgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctga
ggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacc
tcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagc
tgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata