LOCUS BBa_K1075031 1679 bp DNA linear 14-Nov-2024 DEFINITION AraC-pBAD-RBS32-ccdB-TT ACCESSION BBa_K1075031 VERSION BBa_K1075031.1 FEATURES Location/Qualifiers CDS 1..879 /label=AraC misc_feature 909..924 /label=AraO misc_feature 1066..1077 /label=AraO1 promoter 1153..1181 /label=AraPromoter promoter 1..1209 /label=BBa_K808000 RBS 1218..1230 /label=RBS-3\Weak RBS 1224..1227 /label=RBS misc_feature 1218..1230 /label=BBa_B0032 RBS 1218..1230 /label=BBa_B0032 promoter 1..1230 /label=BBa_K1075002 CDS 1237..1542 /label=BBa_C0011 stem_loop 1562..1605 /label=stem_loop misc_feature 1551..1630 /label=BBa_B0010 terminator 1551..1630 /label=BBa_B0010 stem_loop 1646..1665 /label=T7 TE misc_feature 1672..1672 /label=stop polyA_site 1666..1679 /label=polya misc_feature 1639..1679 /label=BBa_B0012 terminator 1639..1679 /label=BBa_B0012 terminator 1551..1679 /label=BBa_B0015 misc_feature 1237..1679 /label=BBa_K1075029 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 tgggctaact actagagtca cacaggaaag tactagatgc agtttaaggt ttacacctat 1261 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc 1321 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt 1381 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg 1441 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat 1501 gacatcaaaa acgccattaa cctgatgttc tggggaatat aatactagag ccaggcatca 1561 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 1621 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //