Promoter(const.)-AraC-Term-pBAD-RBS32 (Arabinose inducable promoter system)
AraC-pBAD is originally found in the genome of e. coli. We combined this part with RBS32.
This is an Arabinose inducable promoter system compromising the repressor AraC and the promoter pBAD as found in the genomic sequence of E. coli. The RBS32 (medium strength) was added downstream of the promoter.
We used the AraC-pBAD construct already characterized by iGEM Darmstadt '12 and added a medium strength RBS (RBS32) to enable direct combination with any coding region.
RBS.3 (medium) -- derivative of BBa_0030
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Double terminator consisting of BBa_B0010 and BBa_B0012
araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30 Khlebnikov A, Skaug T, Keasling JD, modulation of gene expression from the arabinose-inducible araBAD promoter,J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.
This promoter can be induced by arabinose. In presens of arabinose the promotor is active.
Valentina Herbring, Sebastian Palluk, Andreas Schmidt
ccdB is the toxin in a toxin/antitoxin pair. We use it as a default insert in vectors to kill vectors that religate back on themselves in standard assembly. This coding region starts at a later ATG than J08001.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1