BBa_K1075020
1
ssrA(DAS+4
E. coli ssrA(DAS+4)
2013-09-22T11:00:00Z
2015-05-08T01:09:02Z
PCR amplification from gene synthesis
fused to the C terminus of a protein it triggers degradation upon induction of sspB function.
false
false
_1384_
0
12108
9
Not in stock
false
we used the E. coli ssrA tag with a weakened interaction with ClpXP and a linker of 4 amino acids in between to optimize degradation speed increase upon induction with sspB. [1]
false
Max Schelski
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_K1075033
1
BBa_K1075033
RBS32-ccdA-ssrA
2013-09-22T11:00:00Z
2015-05-08T01:09:02Z
a
a
false
false
_1384_
0
16105
9
It's complicated
false
a
false
Marc Schulte
component2358538
1
BBa_B0032
component2358540
1
BBa_K1075020
annotation2358540
1
BBa_K1075020
range2358540
1
22
72
annotation2358538
1
BBa_B0032
range2358538
1
1
13
BBa_K1075020_sequence
1
gcagctaacgatgaaaactacagcgaaaactatgctgacgctagctaataa
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K1075033_sequence
1
tcacacaggaaagtactagaggcagctaacgatgaaaactacagcgaaaactatgctgacgctagctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z