##gff-version 3 ##sequence-region BBa_K1075035 1 666 BBa_K1075035 . CDS 228 470 . + 0 ID=BBa_K1075032;Name=BBa_K1075032 BBa_K1075035 . promoter 1 200 . + 0 ID=BBa_R0010;Name=BBa_R0010 BBa_K1075035 . non_covalent_binding_site 89 126 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_K1075035 . engineered_region 1 200 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_K1075035 . promoter 161 166 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_K1075035 . non_covalent_binding_site 166 200 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_K1075035 . promoter 137 142 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_K1075035 . CDS 1 88 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_K1075035 . start_codon 173 173 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_K1075035 . ribosome_entry_site 209 221 . + 0 ID=BBa_B0032;Name=BBa_B0032 BBa_K1075035 . engineered_region 209 221 . + 0 ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032 BBa_K1075035 . ribosome_entry_site 209 221 . + 0 ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032 BBa_K1075035 . ribosome_entry_site 215 218 . + 0 ID=annotation1710;Name=RBS;Parent=BBa_B0032 BBa_K1075035 . engineered_region 479 666 . + 0 ID=BBa_K1075021;Name=BBa_K1075021 BBa_K1075035 . tag 479 529 . + 0 ID=BBa_K1075020;Name=BBa_K1075020;Parent=BBa_K1075021 BBa_K1075035 . terminator 538 666 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1075021 BBa_K1075035 . terminator 538 617 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1075035 . stem_loop 549 592 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1075035 . engineered_region 538 617 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1075035 . terminator 626 666 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1075035 . stem_loop 633 652 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1075035 . polyA_site 653 666 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1075035 . stop_codon 659 659 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1075035 . engineered_region 626 666 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 >BBa_K1075035 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgaagcagc gcattacagtcaccgtcgacagtgacagctatcagttgctcaaggcatatgatgtcaatatctccggtctggtaagcac caccatgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcagaaaatcaggaagggatggctgaggtcgcc cggtttattgaaatgaacggctcttttgccgatgagaacagggactggtgaaatgcagtttaaggtttacacctatact agaggcagctaacgatgaaaactacagcgaaaactatgctgacgctagctaataatactagagccaggcatcaaataaa acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact ggctcaccttcgggtgggcctttctgcgtttata