BBa_K1075041
1
BBa_K1075041
RBS32-MazE-(Ec)ssrA(DAS+4)
2013-09-22T11:00:00Z
2015-05-08T01:09:03Z
a
a
false
false
_1384_
0
16105
9
It's complicated
false
a
false
Marc Schulte
component2358659
1
BBa_K302032
component2358657
1
BBa_B0032
component2358660
1
BBa_K1075020
annotation2358657
1
BBa_B0032
range2358657
1
1
13
annotation2358659
1
BBa_K302032
range2358659
1
20
268
annotation2358660
1
BBa_K1075020
range2358660
1
277
327
BBa_K1075020
1
ssrA(DAS+4
E. coli ssrA(DAS+4)
2013-09-22T11:00:00Z
2015-05-08T01:09:02Z
PCR amplification from gene synthesis
fused to the C terminus of a protein it triggers degradation upon induction of sspB function.
false
false
_1384_
0
12108
9
Not in stock
false
we used the E. coli ssrA tag with a weakened interaction with ClpXP and a linker of 4 amino acids in between to optimize degradation speed increase upon induction with sspB. [1]
false
Max Schelski
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_K302032
1
BBa_K302032
mazE
2010-10-24T11:00:00Z
2015-05-08T01:11:52Z
''Bacillus Subtilis'' 168 strain
Encodes the liable antitoxin to ''mazF'' in ''Bacillus Subtilis''. It is used in conjungtion with mazF in ''Bacillus Subtilis'' to provide a toxin-antitoxin in various stressful conditions. When transcribtion of both genes is turned off (as both are under contol of same promoter) ''mazE'' will be degraded faster than ''mazF''. There is then no inhibiton of ''mazF'', killing the cell.
false
false
_442_
0
6345
9
Not in stock
false
Sequence isolated based upon the work of Pellegrini, O., Mathy, N., Gogos, A., Shapiro, L., and Condon, C. (2005) The ''Bacillus subtilis'' ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. ''Mol Microbiol'' 56: 1139???1148.
false
Philip Hall
BBa_K1075020_sequence
1
gcagctaacgatgaaaactacagcgaaaactatgctgacgctagctaataa
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K1075041_sequence
1
tcacacaggaaagtactagatgatccacagtagcgtaaagcgttggggaaattcaccggcggtgcggatcccggctacgttaatgcaggcgctcaatctgaatattgatgatgaagtgaagattgacctggtggatggcaaattaattattgagccagtgcgtaaagagcccgtatttacgcttgctgaactggtcaacgacatcacgccggaaaacctccacgagaatatcgactggggagagccgaaagataaggaagtctggtaatactagaggcagctaacgatgaaaactacagcgaaaactatgctgacgctagctaataa
BBa_K302032_sequence
1
atgatccacagtagcgtaaagcgttggggaaattcaccggcggtgcggatcccggctacgttaatgcaggcgctcaatctgaatattgatgatgaagtgaagattgacctggtggatggcaaattaattattgagccagtgcgtaaagagcccgtatttacgcttgctgaactggtcaacgacatcacgccggaaaacctccacgagaatatcgactggggagagccgaaagataaggaagtctggtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z