BBa_K1075045 1 BBa_K1075045 pDawn-RBS34-mCherry-TT 2013-09-22T11:00:00Z 2015-05-08T01:09:03Z a a false false _1384_ 0 16105 9 It's complicated false a false Marc Schulte component2358698 1 BBa_J06702 component2358699 1 BBa_K1075044 annotation2358698 1 BBa_J06702 range2358698 1 1 869 annotation2358699 1 BBa_K1075044 range2358699 1 878 4293 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J06504 1 mCherry monomeric RFP optimized for bacteria 2005-07-17T11:00:00Z 2016-01-25T01:05:28Z mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible Released HQ 2013 mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends. false false _20_ 4206 340 20 In stock false <p> Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. </p> <p> Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick. </p> true ytwang annotation1585833 1 C->T (removing PstI site) range1585833 1 352 352 annotation1585829 1 mCherry range1585829 1 1 711 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K1075044 1 BBa_K1075044 Promoter(const.)-RBS34-YF1-FixJ-FixK-LambdaC-pC (pDawn) 2013-09-22T11:00:00Z 2015-05-08T01:09:03Z PCR amplification from pDawn, triple mutagenesis to remove EcoRI, PstI and XbaI This part can be used to induce transcription of a downstream gene with blue light. false false _1384_ 0 12108 9 It's complicated false pDawn was designed by Moglich et al. [1] false Max Schelski BBa_J06702 1 mCherry mCherry, bacterial with RBS and forward terminator 2005-07-24T11:00:00Z 2015-08-31T04:08:18Z Released HQ 2013 Combines BBa_B0015 forward terminator with BBa_J06602 mCherry, bacterial with RBS false false _20_ 0 340 20 In stock false true Yves Wang component1596432 1 BBa_B0010 component1596427 1 BBa_J06504 component1596419 1 BBa_B0034 component1596442 1 BBa_B0012 annotation1596432 1 BBa_B0010 range1596432 1 741 820 annotation1596442 1 BBa_B0012 range1596442 1 829 869 annotation1596427 1 BBa_J06504 range1596427 1 19 732 annotation1596419 1 BBa_B0034 range1596419 1 1 12 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_J06504_sequence 1 atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa BBa_K1075045_sequence 1 aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcggtcgagatcccggtgcctaatgagtgagctaacttacatcaattgtcaatcgttgagcatgccggcgcgcatcgcgaggcgaaccagctccgaaaggctgttggcctgcatcttggtcatgacgttggcccgatacacctcgatggtgcgcgggctgatgtcgtactcgcgggcgatcagcttgttggaaaggccggcgatcagcccttccatgacctggcgctccctggggctcaacgaggcgacgcgggcggcgatatcctgcgcgacggcctcgctcttggcggccggctcggcctggcggatcgccgattcgatcatggcggtgaggcggtcgtcctcgaaaggcttttccagaaagtccaccgcccctaacttcatcgcctcgaccgcgagcggcacgtcgccgtgaccggtcatgatgaggatcggaaaggggctttgctgcgccttcatccgcttcaacagctcgatgccgtcaaggcccggcatgcgcacgtcggagacgacgcagccgaaggagagacccggcagggcgtcgagaaaggcttgcgcgtcgtcaaacagcgtgacgccgaagccggcagaatccagcaggaaattcagcgaatcccgcatcgccgcgtcgtcgtcgatgacgtagatatgtcccttggtcgtcatgctagacctcctatcatctcgtcggctgccgggagggtgaagcggaaggtcgccccgcccgatgcgttgctctcggcccacatgcgcccgccgtgagcttcgatgatcgagcggctgatggacagtcccacgcccatgccggtgtccttggtggtgaagaaagtctgaaacaggttcggaatgacgtcgtcctggaaaccgctgccggtgtcggacacttcgacctcgatcatgtcgtcggcggcgggggtgttggtgacgacgagctcgcgtcgctgcgactgagccatcgcttccagcgcgttgcggaacaggttgaccaggacctgctggatctgcacccggtcggcgagaacgagatcggcgcccggatcgagactgaagcggagctgcacgttctgctcgcgcgcgccggcaagcccgagcgcgccggcctcctcgatcagcttggagagactctcgacccgcttctccgattcgccgcgggcaacgaagtcgcgcaggcgccggatgatctggccggcgcgcagcgcctgctcggcggcgcggtccagggcgctttcgaccttcggtgtgttcggatcactgctgccggcaagcagccgccgcgagcccttcatgtagttgctgatcgccgccagcggctggttgagctcgtgcgcgagcgcggacgccatttcgcccatggcgctcagcctggagacgtggacgagctcggattgcagttcctggaggcgcgcctgggtctgctggtgctcggtgatatcattctgaataccgacaaaatacgttttatcctctatttccattggatcaatatttaattcattccagaacatcgttccgtcttttttgtagttttggatctgaacggtgaccggttctttattttgtaaagcggttctgatgttgtccacttctggaggatctgtgtgtttcccctgtaagaagcgacagttctttcctaaaatttcctcggtctcgtagccggtcatttgaacaaagccttgatttacgtagacaataggattatcttcaagtgcgggatctgtaattaccacaccgactcgcacgtgatcaagtgcttttttgatgacttccagctgtcctggtatcccaaatgattgaaaactagccacattcaccaccctcaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcaccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctacgcccgtgatcctgatcaccggctatccggacgaaaacatctcgacccgggccgccgaggccggcgtaaaagacgtggttttgaagccgcttctcgacgaaaacctgctcaagcgtatccgccgcgccatccaggaccggcctcgggcatgacctacggggttctacgtaaggcaccccccttaagatatcgctcgaaattttcgaacctcccgataccgcgtaccaatgcgtcatcacaacggagtctcgaaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccgagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc BBa_K1075044_sequence 1 cggtcgagatcccggtgcctaatgagtgagctaacttacatcaattgtcaatcgttgagcatgccggcgcgcatcgcgaggcgaaccagctccgaaaggctgttggcctgcatcttggtcatgacgttggcccgatacacctcgatggtgcgcgggctgatgtcgtactcgcgggcgatcagcttgttggaaaggccggcgatcagcccttccatgacctggcgctccctggggctcaacgaggcgacgcgggcggcgatatcctgcgcgacggcctcgctcttggcggccggctcggcctggcggatcgccgattcgatcatggcggtgaggcggtcgtcctcgaaaggcttttccagaaagtccaccgcccctaacttcatcgcctcgaccgcgagcggcacgtcgccgtgaccggtcatgatgaggatcggaaaggggctttgctgcgccttcatccgcttcaacagctcgatgccgtcaaggcccggcatgcgcacgtcggagacgacgcagccgaaggagagacccggcagggcgtcgagaaaggcttgcgcgtcgtcaaacagcgtgacgccgaagccggcagaatccagcaggaaattcagcgaatcccgcatcgccgcgtcgtcgtcgatgacgtagatatgtcccttggtcgtcatgctagacctcctatcatctcgtcggctgccgggagggtgaagcggaaggtcgccccgcccgatgcgttgctctcggcccacatgcgcccgccgtgagcttcgatgatcgagcggctgatggacagtcccacgcccatgccggtgtccttggtggtgaagaaagtctgaaacaggttcggaatgacgtcgtcctggaaaccgctgccggtgtcggacacttcgacctcgatcatgtcgtcggcggcgggggtgttggtgacgacgagctcgcgtcgctgcgactgagccatcgcttccagcgcgttgcggaacaggttgaccaggacctgctggatctgcacccggtcggcgagaacgagatcggcgcccggatcgagactgaagcggagctgcacgttctgctcgcgcgcgccggcaagcccgagcgcgccggcctcctcgatcagcttggagagactctcgacccgcttctccgattcgccgcgggcaacgaagtcgcgcaggcgccggatgatctggccggcgcgcagcgcctgctcggcggcgcggtccagggcgctttcgaccttcggtgtgttcggatcactgctgccggcaagcagccgccgcgagcccttcatgtagttgctgatcgccgccagcggctggttgagctcgtgcgcgagcgcggacgccatttcgcccatggcgctcagcctggagacgtggacgagctcggattgcagttcctggaggcgcgcctgggtctgctggtgctcggtgatatcattctgaataccgacaaaatacgttttatcctctatttccattggatcaatatttaattcattccagaacatcgttccgtcttttttgtagttttggatctgaacggtgaccggttctttattttgtaaagcggttctgatgttgtccacttctggaggatctgtgtgtttcccctgtaagaagcgacagttctttcctaaaatttcctcggtctcgtagccggtcatttgaacaaagccttgatttacgtagacaataggattatcttcaagtgcgggatctgtaattaccacaccgactcgcacgtgatcaagtgcttttttgatgacttccagctgtcctggtatcccaaatgattgaaaactagccacattcaccaccctcaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcaccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctacgcccgtgatcctgatcaccggctatccggacgaaaacatctcgacccgggccgccgaggccggcgtaaaagacgtggttttgaagccgcttctcgacgaaaacctgctcaagcgtatccgccgcgccatccaggaccggcctcgggcatgacctacggggttctacgtaaggcaccccccttaagatatcgctcgaaattttcgaacctcccgataccgcgtaccaatgcgtcatcacaacggagtctcgaaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccgagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J06702_sequence 1 aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z