LOCUS BBa_K1075046 3919 bp DNA linear 14-Nov-2024 DEFINITION pDawn-RBS32-mazF-TT ACCESSION BBa_K1075046 VERSION BBa_K1075046.1 FEATURES Location/Qualifiers promoter 1..3416 /label=BBa_K1075044 RBS 3425..3437 /label=RBS-3\Weak RBS 3431..3434 /label=RBS misc_feature 3425..3437 /label=BBa_B0032 RBS 3425..3437 /label=BBa_B0032 misc_feature 3777..3782 /label=double TAA added CDS 3444..3782 /label=BBa_K302033 stem_loop 3802..3845 /label=stem_loop misc_feature 3791..3870 /label=BBa_B0010 terminator 3791..3870 /label=BBa_B0010 stem_loop 3886..3905 /label=T7 TE misc_feature 3912..3912 /label=stop polyA_site 3906..3919 /label=polya misc_feature 3879..3919 /label=BBa_B0012 terminator 3879..3919 /label=BBa_B0012 terminator 3791..3919 /label=BBa_B0015 misc_feature 3444..3919 /label=BBa_K1075037 misc_feature 3425..3919 /label=BBa_K1075038 ORIGIN 1 cggtcgagat cccggtgcct aatgagtgag ctaacttaca tcaattgtca atcgttgagc 61 atgccggcgc gcatcgcgag gcgaaccagc tccgaaaggc tgttggcctg catcttggtc 121 atgacgttgg cccgatacac ctcgatggtg cgcgggctga tgtcgtactc gcgggcgatc 181 agcttgttgg aaaggccggc gatcagccct tccatgacct ggcgctccct ggggctcaac 241 gaggcgacgc gggcggcgat atcctgcgcg acggcctcgc tcttggcggc cggctcggcc 301 tggcggatcg ccgattcgat catggcggtg aggcggtcgt cctcgaaagg cttttccaga 361 aagtccaccg cccctaactt catcgcctcg accgcgagcg gcacgtcgcc gtgaccggtc 421 atgatgagga tcggaaaggg gctttgctgc gccttcatcc gcttcaacag ctcgatgccg 481 tcaaggcccg gcatgcgcac gtcggagacg acgcagccga aggagagacc cggcagggcg 541 tcgagaaagg cttgcgcgtc gtcaaacagc gtgacgccga agccggcaga atccagcagg 601 aaattcagcg aatcccgcat cgccgcgtcg tcgtcgatga cgtagatatg tcccttggtc 661 gtcatgctag acctcctatc atctcgtcgg ctgccgggag ggtgaagcgg aaggtcgccc 721 cgcccgatgc gttgctctcg gcccacatgc gcccgccgtg agcttcgatg atcgagcggc 781 tgatggacag tcccacgccc atgccggtgt ccttggtggt gaagaaagtc tgaaacaggt 841 tcggaatgac gtcgtcctgg aaaccgctgc cggtgtcgga cacttcgacc tcgatcatgt 901 cgtcggcggc gggggtgttg gtgacgacga gctcgcgtcg ctgcgactga gccatcgctt 961 ccagcgcgtt gcggaacagg ttgaccagga cctgctggat ctgcacccgg tcggcgagaa 1021 cgagatcggc gcccggatcg agactgaagc ggagctgcac gttctgctcg cgcgcgccgg 1081 caagcccgag cgcgccggcc tcctcgatca gcttggagag actctcgacc cgcttctccg 1141 attcgccgcg ggcaacgaag tcgcgcaggc gccggatgat ctggccggcg cgcagcgcct 1201 gctcggcggc gcggtccagg gcgctttcga ccttcggtgt gttcggatca ctgctgccgg 1261 caagcagccg ccgcgagccc ttcatgtagt tgctgatcgc cgccagcggc tggttgagct 1321 cgtgcgcgag cgcggacgcc atttcgccca tggcgctcag cctggagacg tggacgagct 1381 cggattgcag ttcctggagg cgcgcctggg tctgctggtg ctcggtgata tcattctgaa 1441 taccgacaaa atacgtttta tcctctattt ccattggatc aatatttaat tcattccaga 1501 acatcgttcc gtcttttttg tagttttgga tctgaacggt gaccggttct ttattttgta 1561 aagcggttct gatgttgtcc acttctggag gatctgtgtg tttcccctgt aagaagcgac 1621 agttctttcc taaaatttcc tcggtctcgt agccggtcat ttgaacaaag ccttgattta 1681 cgtagacaat aggattatct tcaagtgcgg gatctgtaat taccacaccg actcgcacgt 1741 gatcaagtgc ttttttgatg acttccagct gtcctggtat cccaaatgat tgaaaactag 1801 ccacattcac caccctcaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg 1861 ttttgcacca ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt 1921 aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca 1981 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg 2041 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg 2101 atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg 2161 tagaggatcg agatctacgc ccgtgatcct gatcaccggc tatccggacg aaaacatctc 2221 gacccgggcc gccgaggccg gcgtaaaaga cgtggttttg aagccgcttc tcgacgaaaa 2281 cctgctcaag cgtatccgcc gcgccatcca ggaccggcct cgggcatgac ctacggggtt 2341 ctacgtaagg cacccccctt aagatatcgc tcgaaatttt cgaacctccc gataccgcgt 2401 accaatgcgt catcacaacg gagtctcgaa aagaggagaa atactagatg agcacaaaaa 2461 agaaaccatt aacacaagag cagcttgagg acgcacgtcg ccttaaagca atttatgaaa 2521 aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc agacaagatg gggatggggc 2581 agtcaggcgt tggtgcttta tttaatggca tcaatgcatt aaatgcttat aacgccgcat 2641 tgcttgcaaa aattctcaaa gttagcgttg aagaatttag cccttcaatc gccagagaaa 2701 tctacgagat gtatgaagcg gttagtatgc agccgtcact tagaagtgag tatgagtacc 2761 ctgttttttc tcatgttcag gcagggatgt tctcacctga gcttagaacc tttaccaaag 2821 gtgatgcgga gagatgggta agcacaacca aaaaagccag tgattctgca ttctggcttg 2881 aggttgaagg taattccatg accgcaccaa caggctccaa gccgagcttt cctgacggaa 2941 tgttaattct cgttgaccct gagcaggctg ttgagccagg tgatttctgc atagccagac 3001 ttgggggtga tgagtttacc ttcaagaaac tgatcaggga tagcggtcag gtgtttttac 3061 aaccactaaa cccacagtac ccaatgatcc catgcaatga gagttgttcc gttgtgggga 3121 aagttatcgc tagtcagtgg cctgaagaga cgtttggcgc tgcaaacgac gaaaactacg 3181 ctttagtagc ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca 3241 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 3301 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 3361 actagagtaa caccgtgcgt gttgactatt ttacctctgg cggtgataat ggttgctact 3421 agagtcacac aggaaagtac tagatggtaa gccgatacgt acccgatatg ggcgatctga 3481 tttgggttga ttttgacccg acaaaaggta gcgagcaagc tggacatcgt ccagctgttg 3541 tcctgagtcc tttcatgtac aacaacaaaa caggtatgtg tctgtgtgtt ccttgtacaa 3601 cgcaatcaaa aggatatccg ttcgaagttg ttttatccgg tcaggaacgt gatggcgtag 3661 cgttagctga tcaggtaaaa agtatcgcct ggcgggcaag aggagcaacg aagaaaggaa 3721 cagttgcccc agaggaatta caactcatta aagccaaaat taacgtactg attgggtaat 3781 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 3841 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 3901 gggcctttct gcgtttata //