LOCUS       BBa_K1075048            2392 bp    DNA     linear       26-Nov-2024
DEFINITION  AraC-pBAD-RBS32-mazF-TT-pLac-RBS32-mazE-(Ec)ssrA(DAS+4)-TT
ACCESSION   BBa_K1075048
VERSION     BBa_K1075048.1  
FEATURES             Location/Qualifiers
     CDS             1..879
                     /label=AraC
     misc_feature    909..924
                     /label=AraO
     misc_feature    1066..1077
                     /label=AraO1
     promoter        1153..1181
                     /label=AraPromoter
     promoter        1..1209
                     /label=BBa_K808000
     RBS             1218..1230
                     /label=RBS-3\Weak
     RBS             1224..1227
                     /label=RBS
     misc_feature    1218..1230
                     /label=BBa_B0032
     RBS             1218..1230
                     /label=BBa_B0032
     misc_feature    1570..1575
                     /label=double TAA added
     CDS             1237..1575
                     /label=BBa_K302033
     stem_loop       1595..1638
                     /label=stem_loop
     misc_feature    1584..1663
                     /label=BBa_B0010
     terminator      1584..1663
                     /label=BBa_B0010
     stem_loop       1679..1698
                     /label=T7 TE
     misc_feature    1705..1705
                     /label=stop
     polyA_site      1699..1712
                     /label=polya
     misc_feature    1672..1712
                     /label=BBa_B0012
     terminator      1672..1712
                     /label=BBa_B0012
     terminator      1584..1712
                     /label=BBa_B0015
     misc_feature    1237..1712
                     /label=BBa_K1075037
     misc_feature    1218..1712
                     /label=BBa_K1075038
     misc_feature    1..1712
                     /label=BBa_K1075040
     CDS             1721..1808
                     /label=end of LacI coding region (inactive)
     misc_feature    1721..1920
                     /label=BBa_R0010
     misc_feature    1809..1846
                     /label=CAP binding site
     promoter        1857..1862
                     /label=-35
     promoter        1881..1886
                     /label=-10
     misc_feature    1886..1920
                     /label=LacI binding site
     misc_feature    1893..1893
                     /label=start
     promoter        1721..1920
                     /label=BBa_R0010
     RBS             1929..1941
                     /label=RBS-3\Weak
     RBS             1935..1938
                     /label=RBS
     misc_feature    1929..1941
                     /label=BBa_B0032
     RBS             1929..1941
                     /label=BBa_B0032
     CDS             1948..2196
                     /label=BBa_K302032
     misc_feature    2205..2255
                     /label=BBa_K1075020
     misc_feature    1929..2255
                     /label=BBa_K1075041
     stem_loop       2275..2318
                     /label=stem_loop
     misc_feature    2264..2343
                     /label=BBa_B0010
     terminator      2264..2343
                     /label=BBa_B0010
     stem_loop       2359..2378
                     /label=T7 TE
     misc_feature    2385..2385
                     /label=stop
     polyA_site      2379..2392
                     /label=polya
     misc_feature    2352..2392
                     /label=BBa_B0012
     terminator      2352..2392
                     /label=BBa_B0012
     terminator      2264..2392
                     /label=BBa_B0015
     misc_feature    1721..2392
                     /label=BBa_K1075043
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 tgggctaact actagagtca cacaggaaag tactagatgg taagccgata cgtacccgat
     1261 atgggcgatc tgatttgggt tgattttgac ccgacaaaag gtagcgagca agctggacat
     1321 cgtccagctg ttgtcctgag tcctttcatg tacaacaaca aaacaggtat gtgtctgtgt
     1381 gttccttgta caacgcaatc aaaaggatat ccgttcgaag ttgttttatc cggtcaggaa
     1441 cgtgatggcg tagcgttagc tgatcaggta aaaagtatcg cctggcgggc aagaggagca
     1501 acgaagaaag gaacagttgc cccagaggaa ttacaactca ttaaagccaa aattaacgta
     1561 ctgattgggt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1621 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1681 ctcaccttcg ggtgggcctt tctgcgttta tatactagag caatacgcaa accgcctctc
     1741 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     1801 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     1861 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     1921 tactagagtc acacaggaaa gtactagatg atccacagta gcgtaaagcg ttggggaaat
     1981 tcaccggcgg tgcggatccc ggctacgtta atgcaggcgc tcaatctgaa tattgatgat
     2041 gaagtgaaga ttgacctggt ggatggcaaa ttaattattg agccagtgcg taaagagccc
     2101 gtatttacgc ttgctgaact ggtcaacgac atcacgccgg aaaacctcca cgagaatatc
     2161 gactggggag agccgaaaga taaggaagtc tggtaatact agaggcagct aacgatgaaa
     2221 actacagcga aaactatgct gacgctagct aataatacta gagccaggca tcaaataaaa
     2281 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2341 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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