LOCUS BBa_K1075049 3038 bp DNA linear 29-Nov-2024 DEFINITION AraC-pBAD-RBS32-sspB-TT-pLac-RBS34-mCherry-TT ACCESSION BBa_K1075049 VERSION BBa_K1075049.1 FEATURES Location/Qualifiers promoter 1..1291 /label=BBa_K1075001 RBS 1300..1312 /label=RBS-3\Weak RBS 1306..1309 /label=RBS misc_feature 1300..1312 /label=BBa_B0032 RBS 1300..1312 /label=BBa_B0032 CDS 1319..1816 /label=sspB CDS 1319..1816 /label=BBa_K174000 stem_loop 1836..1879 /label=stem_loop misc_feature 1825..1904 /label=BBa_B0010 terminator 1825..1904 /label=BBa_B0010 stem_loop 1920..1939 /label=T7 TE misc_feature 1946..1946 /label=stop polyA_site 1940..1953 /label=polya misc_feature 1913..1953 /label=BBa_B0012 terminator 1913..1953 /label=BBa_B0012 terminator 1825..1953 /label=BBa_B0015 misc_feature 1319..1953 /label=BBa_K1075004 misc_feature 1300..1953 /label=BBa_K1075005 misc_feature 1..1953 /label=BBa_K1075007 CDS 1962..2049 /label=end of LacI coding region (inactive) misc_feature 1962..2161 /label=BBa_R0010 misc_feature 2050..2087 /label=CAP binding site promoter 2098..2103 /label=-35 promoter 2122..2127 /label=-10 misc_feature 2127..2161 /label=LacI binding site misc_feature 2134..2134 /label=start promoter 1962..2161 /label=BBa_R0010 misc_feature 2174..2177 /label=conserved RBS 2170..2181 /label=BBa_B0034 CDS 2188..2898 /label=mCherry misc_feature 2539..2539 /label=C->T (removing PstI site) misc_feature 2188..2901 /label=BBa_J06504 stem_loop 2921..2964 /label=stem_loop misc_feature 2910..2989 /label=BBa_B0010 terminator 2910..2989 /label=BBa_B0010 stem_loop 3005..3024 /label=T7 TE misc_feature 3031..3031 /label=stop polyA_site 3025..3038 /label=polya misc_feature 2998..3038 /label=BBa_B0012 terminator 2998..3038 /label=BBa_B0012 misc_feature 1962..3038 /label=BBa_K204044 ORIGIN 1 tgcataatgt gcctgtcaaa tggacgaagc agggattctg caaaccctat gctactccgt 61 caagccgtca attgtctgat tcgttaccaa ttatgacaac ttgacggcta catcattcac 121 tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac 181 ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat 241 ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa 301 gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac 361 atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg 421 acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat 481 gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc 541 cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc 601 atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca 661 gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg 721 accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa 781 ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc 841 tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt 901 taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc 961 ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat 1021 cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc 1081 gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa 1141 aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg 1201 ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca 1261 actctctact gtttctccat acccgttttt ttactagagt cacacaggaa agtactagat 1321 ggatttgtca cagctaacac cacgtcgtcc ctatctgctg cgtgcattct atgagtggtt 1381 gctggataac cagctcacgc cgcacctggt ggtggatgtg acgctccctg gcgtgcaggt 1441 tcctatggaa tatgcgcgtg acgggcaaat cgtactcaac attgcgccgc gtgctgtcgg 1501 caatctggaa ctggcgaatg atgaggtgcg ctttaacgcg cgctttggtg gcattccgcg 1561 tcaggtttct gtgccgctgg ctgccgtgct ggctatctac gcccgtgaaa atggcgcagg 1621 cacgatgttt gagcctgaag ctgcctacga tgaagatacc agcatcatga atgatgaaga 1681 ggcatcggca gacaacgaaa ccgttatgtc ggttattgat ggcgacaagc cagatcacga 1741 tgatgacact catcctgacg atgaacctcc gcagccacca cgcggtggtc gaccggcatt 1801 acgcgttgtg aagtaatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 1861 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 1921 gctcaccttc gggtgggcct ttctgcgttt atatactaga gcaatacgca aaccgcctct 1981 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 2041 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt 2101 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac 2161 atactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagga taacatggcc 2221 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag 2281 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 2341 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg 2401 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc 2461 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc 2521 gtgacccagg actcctcctt gcaggacggc gagttcatct acaaggtgaa gctgcgcggc 2581 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 2641 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag 2701 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc 2761 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag 2821 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg 2881 gacgagctgt acaagtaata atactagagc caggcatcaa ataaaacgaa aggctcagtc 2941 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 3001 cactggctca ccttcgggtg ggcctttctg cgtttata //