##gff-version 3
##sequence-region BBa_K1075049 1 3038
BBa_K1075049	.	engineered_region	1962	3038	.	+	0	ID=BBa_K204044;Name=BBa_K204044
BBa_K1075049	.	promoter	1962	2161	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K204044
BBa_K1075049	.	non_covalent_binding_site	2050	2087	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1075049	.	non_covalent_binding_site	2127	2161	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1075049	.	promoter	2098	2103	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1075049	.	start_codon	2134	2134	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1075049	.	engineered_region	1962	2161	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1075049	.	CDS	1962	2049	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1075049	.	promoter	2122	2127	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1075049	.	terminator	2910	2989	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K204044
BBa_K1075049	.	stem_loop	2921	2964	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1075049	.	engineered_region	2910	2989	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1075049	.	engineered_region	2188	2901	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_K204044
BBa_K1075049	.	sequence_alteration	2539	2539	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K1075049	.	CDS	2188	2898	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K1075049	.	terminator	2998	3038	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K204044
BBa_K1075049	.	stop_codon	3031	3031	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1075049	.	polyA_site	3025	3038	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1075049	.	stem_loop	3005	3024	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1075049	.	engineered_region	2998	3038	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1075049	.	ribosome_entry_site	2170	2181	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K204044
BBa_K1075049	.	conserved_region	2174	2177	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1075049	.	engineered_region	1	1953	.	+	0	ID=BBa_K1075007;Name=BBa_K1075007
BBa_K1075049	.	promoter	1	1291	.	+	0	ID=BBa_K1075001;Name=BBa_K1075001;Parent=BBa_K1075007
BBa_K1075049	.	engineered_region	1300	1953	.	+	0	ID=BBa_K1075005;Name=BBa_K1075005;Parent=BBa_K1075007
BBa_K1075049	.	ribosome_entry_site	1300	1312	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1075005
BBa_K1075049	.	engineered_region	1300	1312	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1075049	.	ribosome_entry_site	1300	1312	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1075049	.	ribosome_entry_site	1306	1309	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1075049	.	engineered_region	1319	1953	.	+	0	ID=BBa_K1075004;Name=BBa_K1075004;Parent=BBa_K1075005
BBa_K1075049	.	terminator	1825	1953	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1075004
BBa_K1075049	.	terminator	1825	1904	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1075049	.	stem_loop	1836	1879	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1075049	.	engineered_region	1825	1904	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1075049	.	terminator	1913	1953	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1075049	.	stop_codon	1946	1946	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1075049	.	polyA_site	1940	1953	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1075049	.	stem_loop	1920	1939	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1075049	.	engineered_region	1913	1953	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1075049	.	CDS	1319	1816	.	+	0	ID=BBa_K174000;Name=BBa_K174000;Parent=BBa_K1075004
BBa_K1075049	.	CDS	1319	1816	.	+	0	ID=annotation2027215;Name=sspB;Parent=BBa_K174000
>BBa_K1075049
tgcataatgtgcctgtcaaatggacgaagcagggattctgcaaaccctatgctactccgtcaagccgtcaattgtctga
ttcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctgg
ccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgatagg
catccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaac
tgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgt
ctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgc
ttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcg
ttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaata
ttgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctc
cggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccc
tgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaa
aatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcagggg
atcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacat
tgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaa
gcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgca
cggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactc
tctactgtttctccatacccgtttttttactagagtcacacaggaaagtactagatggatttgtcacagctaacaccac
gtcgtccctatctgctgcgtgcattctatgagtggttgctggataaccagctcacgccgcacctggtggtggatgtgac
gctccctggcgtgcaggttcctatggaatatgcgcgtgacgggcaaatcgtactcaacattgcgccgcgtgctgtcggc
aatctggaactggcgaatgatgaggtgcgctttaacgcgcgctttggtggcattccgcgtcaggtttctgtgccgctgg
ctgccgtgctggctatctacgcccgtgaaaatggcgcaggcacgatgtttgagcctgaagctgcctacgatgaagatac
cagcatcatgaatgatgaagaggcatcggcagacaacgaaaccgttatgtcggttattgatggcgacaagccagatcac
gatgatgacactcatcctgacgatgaacctccgcagccaccacgcggtggtcgaccggcattacgcgttgtgaagtaat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccg
cctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgca
acgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgg
aattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggata
acatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcga
gggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttc
gcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactact
tgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgaccca
ggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgta
atgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatca
agcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgca
gctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtac
gaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttata