LOCUS       BBa_K1075051             702 bp    DNA     linear       26-Nov-2024
DEFINITION  pLac-RBS34-mazF-TT - inducible Killswitch
ACCESSION   BBa_K1075051
VERSION     BBa_K1075051.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    560..565
                     /label=double TAA added
     CDS             227..565
                     /label=BBa_K302033
     stem_loop       585..628
                     /label=stem_loop
     misc_feature    574..653
                     /label=BBa_B0010
     terminator      574..653
                     /label=BBa_B0010
     stem_loop       669..688
                     /label=T7 TE
     misc_feature    695..695
                     /label=stop
     polyA_site      689..702
                     /label=polya
     misc_feature    662..702
                     /label=BBa_B0012
     terminator      662..702
                     /label=BBa_B0012
     terminator      574..702
                     /label=BBa_B0015
     misc_feature    227..702
                     /label=BBa_K1075037
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg taagccgata
      241 cgtacccgat atgggcgatc tgatttgggt tgattttgac ccgacaaaag gtagcgagca
      301 agctggacat cgtccagctg ttgtcctgag tcctttcatg tacaacaaca aaacaggtat
      361 gtgtctgtgt gttccttgta caacgcaatc aaaaggatat ccgttcgaag ttgttttatc
      421 cggtcaggaa cgtgatggcg tagcgttagc tgatcaggta aaaagtatcg cctggcgggc
      481 aagaggagca acgaagaaag gaacagttgc cccagaggaa ttacaactca ttaaagccaa
      541 aattaacgta ctgattgggt aataatacta gagccaggca tcaaataaaa cgaaaggctc
      601 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
      661 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//